MAMP (microbe-associated molecular pattern)-induced changes in
plasma membrane-associated proteins

J 2017

MAMP (microbe-associated molecular pattern)-induced changes in plasma membrane-associated proteins

UHLÍKOVÁ, Hana, Martin SOLANSKÝ, Vendula HRDINOVÁ, Ondrej ŠEDO, Tomáš KAŠPAROVSKÝ et. al.

Základní údaje

Originální název

MAMP (microbe-associated molecular pattern)-induced changes in plasma membrane-associated proteins

Autoři

UHLÍKOVÁ, Hana (203 Česká republika, domácí), Martin SOLANSKÝ (203 Česká republika, domácí), Vendula HRDINOVÁ (203 Česká republika, domácí), Ondrej ŠEDO (203 Česká republika, domácí), Tomáš KAŠPAROVSKÝ (203 Česká republika, domácí), Jan HEJÁTKO (203 Česká republika, domácí) a Jan LOCHMAN (203 Česká republika, garant, domácí)

Vydání

Journal of Plant Physiology, JENA, ELSEVIER GMBH, 2017, 0176-1617

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.833

Kód RIV

RIV/00216224:14310/17:00095532

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.jplph.2016.12.005

UT WoS

000397471200006

Klíčová slova anglicky

Cryptogein; Rab8; Resistance; ATPase; NADP-malic enzyme; Glutamine synthetase

Štítky

CF PROT, NZ

Změněno: 31. 5. 2022 17:06, RNDr. Pavel Šmerk, Ph.D.

Anotace

V originále

Plant plasma membrane associated proteins play significant roles in Microbe-Associated Molecular Pattern (MAMP) mediated defence responses including signal transduction, membrane transport or energetic metabolism. To elucidate the dynamics of proteins associated with plasma membrane in response to cryptogein, a well-known MAMP of defence reaction secreted by the oomycete Phytophthora cryptogea, 2D-Blue Native/SDS gel electrophoresis of plasma membrane fractions was employed. This approach revealed 21 up- or down-regulated protein spots of which 15 were successfully identified as proteins related to transport through plasma membrane, vesicle trafficking, and metabolic enzymes including cytosolic NADP-malic enzyme and glutamine synthetase. Observed changes in proteins were also confirmed on transcriptional level by qRT-PCR analysis. In addition, a significantly decreased accumulation of transcripts observed after employment of a mutant variant of cryptogein Leu41Phe, exhibiting a conspicuous defect in induction of resistance, sustains the contribution of identified proteins in cryptogein-triggered cellular responses. Our data provide further evidence for dynamic MAMP-induced changes in plasma membrane associated proteins. (C) 2016 Elsevier GmbH. All rights reserved.

Návaznosti

GAP501/12/0590, projekt VaV

Název: Charakterizace procesů zapojených v indukci resistence rostlin na patogeny s využitím elicitinů se změněnou schopností vyvolávat obranné reakce

Investor: Grantová agentura ČR, Charakterizace procesů zapojených v indukci resistence rostlin na patogeny s využitím elicitinů se změněnou schopností vyvolávat obranné reakce

LM2015043, projekt VaV

Název: Česká infrastruktura pro integrativní strukturní biologii (Akronym: CIISB)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech Infrastructure for Integrative Structural Biology

Citovat

UHLÍKOVÁ, Hana, Martin SOLANSKÝ, Vendula HRDINOVÁ, Ondrej ŠEDO, Tomáš KAŠPAROVSKÝ, Jan HEJÁTKO a Jan LOCHMAN. MAMP (microbe-associated molecular pattern)-induced changes in plasma membrane-associated proteins. Journal of Plant Physiology. JENA: ELSEVIER GMBH, 2017, roč. 210, March, s. 51-57. ISSN 0176-1617. Dostupné z: https://dx.doi.org/10.1016/j.jplph.2016.12.005.

@article{1409620,
   author = {Uhlíková, Hana and Solanský, Martin and Hrdinová, Vendula and Šedo, Ondrej and Kašparovský, Tomáš and Hejátko, Jan and Lochman, Jan},
   article_location = {JENA},
   article_number = {March},
   doi = {http://dx.doi.org/10.1016/j.jplph.2016.12.005},
   keywords = {Cryptogein; Rab8; Resistance; ATPase; NADP-malic enzyme; Glutamine synthetase},
   language = {eng},
   issn = {0176-1617},
   journal = {Journal of Plant Physiology},
   note = {Nejde o článek v odborném periodioku. WoS. Document Type:Editorial Material. Nutné odvést do RIV jko výsledek typu "O" - ostatní.},
   title = {MAMP (microbe-associated molecular pattern)-induced changes in plasma membrane-associated proteins},
   url = {http://dx.doi.org/10.1016/j.jplph.2016.12.005},
   volume = {210},
   year = {2017}
}

TY  - JOUR
ID  - 1409620
AU  - Uhlíková, Hana - Solanský, Martin - Hrdinová, Vendula - Šedo, Ondrej - Kašparovský, Tomáš - Hejátko, Jan - Lochman, Jan
PY  - 2017
TI  - MAMP (microbe-associated molecular pattern)-induced changes in plasma membrane-associated proteins
JF  - Journal of Plant Physiology
VL  - 210
IS  - March
SP  - 51-57
EP  - 51-57
PB  - ELSEVIER GMBH
SN  - 01761617
N1  - Nejde o článek v odborném periodioku. WoS. Document Type:Editorial Material. Nutné odvést do RIV jko výsledek typu "O" - ostatní.
KW  - Cryptogein
KW  - Rab8
KW  - Resistance
KW  - ATPase
KW  - NADP-malic enzyme
KW  - Glutamine synthetase
UR  - http://dx.doi.org/10.1016/j.jplph.2016.12.005
N2  - Plant plasma membrane associated proteins play significant roles in Microbe-Associated Molecular Pattern (MAMP) mediated defence responses including signal transduction, membrane transport or energetic metabolism. To elucidate the dynamics of proteins associated with plasma membrane in response to cryptogein, a well-known MAMP of defence reaction secreted by the oomycete Phytophthora cryptogea, 2D-Blue Native/SDS gel electrophoresis of plasma membrane fractions was employed. This approach revealed 21 up- or down-regulated protein spots of which 15 were successfully identified as proteins related to transport through plasma membrane, vesicle trafficking, and metabolic enzymes including cytosolic NADP-malic enzyme and glutamine synthetase. Observed changes in proteins were also confirmed on transcriptional level by qRT-PCR analysis. In addition, a significantly decreased accumulation of transcripts observed after employment of a mutant variant of cryptogein Leu41Phe, exhibiting a conspicuous defect in induction of resistance, sustains the contribution of identified proteins in cryptogein-triggered cellular responses. Our data provide further evidence for dynamic MAMP-induced changes in plasma membrane associated proteins. (C) 2016 Elsevier GmbH. All rights reserved.
ER  -

UHLÍKOVÁ, Hana, Martin SOLANSKÝ, Vendula HRDINOVÁ, Ondrej ŠEDO, Tomáš KAŠPAROVSKÝ, Jan HEJÁTKO a Jan LOCHMAN. MAMP (microbe-associated molecular pattern)-induced changes in plasma membrane-associated proteins. \textit{Journal of Plant Physiology}. JENA: ELSEVIER GMBH, 2017, roč.~210, March, s.~51-57. ISSN~0176-1617. Dostupné z: https://dx.doi.org/10.1016/j.jplph.2016.12.005.

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