| DŽATKO, Šimon, Michaela KRAFČÍKOVÁ, R. HANSEL-HERTSCH, T. FESSL, Radovan FIALA, Tomáš LOJA, Daniel KRAFČÍK, J.L. MERGNY, Silvie TRANTÍRKOVÁ and Lukáš TRANTÍREK. Evaluation of the Stability of DNA i-Motifs in the Nuclei of Living Mammalian Cells. Angewandte Chemie International Edition. WEINHEIM (GERMANY): Verlag Chemie, 2018, vol. 57, No 8, p. 2165-2169. ISSN 1433-7851. Available from: https://dx.doi.org/10.1002/anie.201712284. |
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@article{1409989,
author = {Džatko, Šimon and Krafčíková, Michaela and HanselandHertsch, R. and Fessl, T. and Fiala, Radovan and Loja, Tomáš and Krafčík, Daniel and Mergny, J.L. and Trantírková, Silvie and Trantírek, Lukáš},
article_location = {WEINHEIM (GERMANY)},
article_number = {8},
doi = {http://dx.doi.org/10.1002/anie.201712284},
keywords = {DNA; i-motifs; in-cell NMR spectroscopy; structural biology},
language = {eng},
issn = {1433-7851},
journal = {Angewandte Chemie International Edition},
title = {Evaluation of the Stability of DNA i-Motifs in the Nuclei of Living Mammalian Cells},
volume = {57},
year = {2018}
}
TY - JOUR
ID - 1409989
AU - Džatko, Šimon - Krafčíková, Michaela - Hansel-Hertsch, R. - Fessl, T. - Fiala, Radovan - Loja, Tomáš - Krafčík, Daniel - Mergny, J.L. - Trantírková, Silvie - Trantírek, Lukáš
PY - 2018
TI - Evaluation of the Stability of DNA i-Motifs in the Nuclei of Living Mammalian Cells
JF - Angewandte Chemie International Edition
VL - 57
IS - 8
SP - 2165-2169
EP - 2165-2169
PB - Verlag Chemie
SN - 14337851
KW - DNA
KW - i-motifs
KW - in-cell NMR spectroscopy
KW - structural biology
N2 - C-rich DNA has the capacity to form a tetra-stranded structure known as an i-motif. The i-motifs within genomic DNA have been proposed to contribute to the regulation of DNA transcription. However, direct experimental evidence for the existence of these structures invivo has been missing. Whether i-motif structures form in complex environment of living cells is not currently known. Herein, using state-of-the-art in-cell NMR spectroscopy, we evaluate the stabilities of i-motif structures in the complex cellular environment. We show that i-motifs formed from naturally occurring C-rich sequences in the human genome are stable and persist in the nuclei of living human cells. Our data show that i-motif stabilities invivo are generally distinct from those invitro. Our results are the first to interlink the stability of DNA i-motifs invitro with their stability invivo and provide essential information for the design and development of i-motif-based DNA biosensors for intracellular applications.
ER -
DŽATKO, Šimon, Michaela KRAFČÍKOVÁ, R. HANSEL-HERTSCH, T. FESSL, Radovan FIALA, Tomáš LOJA, Daniel KRAFČÍK, J.L. MERGNY, Silvie TRANTÍRKOVÁ and Lukáš TRANTÍREK. Evaluation of the Stability of DNA i-Motifs in the Nuclei of Living Mammalian Cells. \textit{Angewandte Chemie International Edition}. WEINHEIM (GERMANY): Verlag Chemie, 2018, vol.~57, No~8, p.~2165-2169. ISSN~1433-7851. Available from: https://dx.doi.org/10.1002/anie.201712284.
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