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@article{1410075, author = {Smerkova, Kristyna and Vaculovič, Tomáš and Vaculovičová, Markéta and Kynicky, Jindrich and Brtnicky, Martin and Eckschlager, Tomas and Stiborova, Marie and Hubalek, Jaromir and Adam, Vojtěch}, article_location = {San Diego}, article_number = {DEC}, doi = {http://dx.doi.org/10.1016/j.ab.2017.09.018}, keywords = {DNA; Platinum based cytostatics; Sequencing; Mass spectrometry}, language = {eng}, issn = {0003-2697}, journal = {Analytical Biochemistry}, title = {DNA interaction with platinum-based cytostatics revealed by DNA sequencing}, url = {https://www.sciencedirect.com/science/article/pii/S0003269717303846?via%3Dihub}, volume = {539}, year = {2017} }
TY - JOUR ID - 1410075 AU - Smerkova, Kristyna - Vaculovič, Tomáš - Vaculovičová, Markéta - Kynicky, Jindrich - Brtnicky, Martin - Eckschlager, Tomas - Stiborova, Marie - Hubalek, Jaromir - Adam, Vojtěch PY - 2017 TI - DNA interaction with platinum-based cytostatics revealed by DNA sequencing JF - Analytical Biochemistry VL - 539 IS - DEC SP - 22-28 EP - 22-28 PB - ACADEMIC PRESS INC ELSEVIER SCIENCE SN - 00032697 KW - DNA KW - Platinum based cytostatics KW - Sequencing KW - Mass spectrometry UR - https://www.sciencedirect.com/science/article/pii/S0003269717303846?via%3Dihub L2 - https://www.sciencedirect.com/science/article/pii/S0003269717303846?via%3Dihub N2 - The main mechanism of action of platinum-based cytostatic drugs cisplatin, oxaliplatin and carboplatin - is the formation of DNA cross-links, which restricts the transcription due to the disability of DNA to enter the active site of the polymerase. The polymerase chain reaction (PCR) was employed as a simplified model of the amplification process in the cell nucleus. PCR with fluorescently labelled dideoxynucleotides commonly employed for DNA sequencing was used to monitor the effect of platinum-based cytostatics on DNA in terms of decrease in labeling efficiency dependent on a presence of the DNA-drug cross-link. It was found that significantly different amounts of the drugs - cisplatin (0.21 mu g/mL), oxaliplatin (5.23 mu g/mL), and carboplatin (71.11 mu g/mL) - were required to cause the same quenching effect (50%) on the fluorescent labelling of 50 mu g/mL of DNA. Moreover, it was found that even though the amounts of the drugs was applied to the reaction mixture differing by several orders of magnitude, the amount of incorporated platinum, quantified by inductively coupled plasma mass spectrometry, was in all cases at the level of tenths of mu g per 5 mu g of DNA. ER -
SMERKOVA, Kristyna, Tomáš VACULOVIČ, Markéta VACULOVIČOVÁ, Jindrich KYNICKY, Martin BRTNICKY, Tomas ECKSCHLAGER, Marie STIBOROVA, Jaromir HUBALEK a Vojtěch ADAM. DNA interaction with platinum-based cytostatics revealed by DNA sequencing. \textit{Analytical Biochemistry}. San Diego: ACADEMIC PRESS INC ELSEVIER SCIENCE, 2017, roč.~539, DEC, s.~22-28. ISSN~0003-2697. Dostupné z: https://dx.doi.org/10.1016/j.ab.2017.09.018.
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