DNA interaction with platinum-based cytostatics revealed by DNA
sequencing

J 2017

DNA interaction with platinum-based cytostatics revealed by DNA sequencing

SMERKOVA, Kristyna, Tomáš VACULOVIČ, Markéta VACULOVIČOVÁ, Jindrich KYNICKY, Martin BRTNICKY et. al.

Základní údaje

Originální název

DNA interaction with platinum-based cytostatics revealed by DNA sequencing

Autoři

SMERKOVA, Kristyna (203 Česká republika), Tomáš VACULOVIČ (203 Česká republika, domácí), Markéta VACULOVIČOVÁ (203 Česká republika), Jindrich KYNICKY (203 Česká republika), Martin BRTNICKY (203 Česká republika), Tomas ECKSCHLAGER (203 Česká republika), Marie STIBOROVA (203 Česká republika), Jaromir HUBALEK (203 Česká republika) a Vojtěch ADAM (203 Česká republika, garant)

Vydání

Analytical Biochemistry, San Diego, ACADEMIC PRESS INC ELSEVIER SCIENCE, 2017, 0003-2697

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Full Text

Impakt faktor

Impact factor: 2.275

Kód RIV

RIV/00216224:14310/17:00100210

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.ab.2017.09.018

UT WoS

000417117000004

Klíčová slova anglicky

DNA; Platinum based cytostatics; Sequencing; Mass spectrometry

Štítky

NZ, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 17. 12. 2019 14:27, Mgr. Marie Šípková, DiS.

Anotace

V originále

The main mechanism of action of platinum-based cytostatic drugs cisplatin, oxaliplatin and carboplatin - is the formation of DNA cross-links, which restricts the transcription due to the disability of DNA to enter the active site of the polymerase. The polymerase chain reaction (PCR) was employed as a simplified model of the amplification process in the cell nucleus. PCR with fluorescently labelled dideoxynucleotides commonly employed for DNA sequencing was used to monitor the effect of platinum-based cytostatics on DNA in terms of decrease in labeling efficiency dependent on a presence of the DNA-drug cross-link. It was found that significantly different amounts of the drugs - cisplatin (0.21 mu g/mL), oxaliplatin (5.23 mu g/mL), and carboplatin (71.11 mu g/mL) - were required to cause the same quenching effect (50%) on the fluorescent labelling of 50 mu g/mL of DNA. Moreover, it was found that even though the amounts of the drugs was applied to the reaction mixture differing by several orders of magnitude, the amount of incorporated platinum, quantified by inductively coupled plasma mass spectrometry, was in all cases at the level of tenths of mu g per 5 mu g of DNA.

Návaznosti

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

SMERKOVA, Kristyna, Tomáš VACULOVIČ, Markéta VACULOVIČOVÁ, Jindrich KYNICKY, Martin BRTNICKY, Tomas ECKSCHLAGER, Marie STIBOROVA, Jaromir HUBALEK a Vojtěch ADAM. DNA interaction with platinum-based cytostatics revealed by DNA sequencing. Analytical Biochemistry. San Diego: ACADEMIC PRESS INC ELSEVIER SCIENCE, 2017, roč. 539, DEC, s. 22-28. ISSN 0003-2697. Dostupné z: https://dx.doi.org/10.1016/j.ab.2017.09.018.

@article{1410075,
   author = {Smerkova, Kristyna and Vaculovič, Tomáš and Vaculovičová, Markéta and Kynicky, Jindrich and Brtnicky, Martin and Eckschlager, Tomas and Stiborova, Marie and Hubalek, Jaromir and Adam, Vojtěch},
   article_location = {San Diego},
   article_number = {DEC},
   doi = {http://dx.doi.org/10.1016/j.ab.2017.09.018},
   keywords = {DNA; Platinum based cytostatics; Sequencing; Mass spectrometry},
   language = {eng},
   issn = {0003-2697},
   journal = {Analytical Biochemistry},
   title = {DNA interaction with platinum-based cytostatics revealed by DNA sequencing},
   url = {https://www.sciencedirect.com/science/article/pii/S0003269717303846?via%3Dihub},
   volume = {539},
   year = {2017}
}

TY  - JOUR
ID  - 1410075
AU  - Smerkova, Kristyna - Vaculovič, Tomáš - Vaculovičová, Markéta - Kynicky, Jindrich - Brtnicky, Martin - Eckschlager, Tomas - Stiborova, Marie - Hubalek, Jaromir - Adam, Vojtěch
PY  - 2017
TI  - DNA interaction with platinum-based cytostatics revealed by DNA sequencing
JF  - Analytical Biochemistry
VL  - 539
IS  - DEC
SP  - 22-28
EP  - 22-28
PB  - ACADEMIC PRESS INC ELSEVIER SCIENCE
SN  - 00032697
KW  - DNA
KW  - Platinum based cytostatics
KW  - Sequencing
KW  - Mass spectrometry
UR  - https://www.sciencedirect.com/science/article/pii/S0003269717303846?via%3Dihub
L2  - https://www.sciencedirect.com/science/article/pii/S0003269717303846?via%3Dihub
N2  - The main mechanism of action of platinum-based cytostatic drugs cisplatin, oxaliplatin and carboplatin - is the formation of DNA cross-links, which restricts the transcription due to the disability of DNA to enter the active site of the polymerase. The polymerase chain reaction (PCR) was employed as a simplified model of the amplification process in the cell nucleus. PCR with fluorescently labelled dideoxynucleotides commonly employed for DNA sequencing was used to monitor the effect of platinum-based cytostatics on DNA in terms of decrease in labeling efficiency dependent on a presence of the DNA-drug cross-link. It was found that significantly different amounts of the drugs - cisplatin (0.21 mu g/mL), oxaliplatin (5.23 mu g/mL), and carboplatin (71.11 mu g/mL) - were required to cause the same quenching effect (50%) on the fluorescent labelling of 50 mu g/mL of DNA. Moreover, it was found that even though the amounts of the drugs was applied to the reaction mixture differing by several orders of magnitude, the amount of incorporated platinum, quantified by inductively coupled plasma mass spectrometry, was in all cases at the level of tenths of mu g per 5 mu g of DNA.
ER  -

SMERKOVA, Kristyna, Tomáš VACULOVIČ, Markéta VACULOVIČOVÁ, Jindrich KYNICKY, Martin BRTNICKY, Tomas ECKSCHLAGER, Marie STIBOROVA, Jaromir HUBALEK a Vojtěch ADAM. DNA interaction with platinum-based cytostatics revealed by DNA sequencing. \textit{Analytical Biochemistry}. San Diego: ACADEMIC PRESS INC ELSEVIER SCIENCE, 2017, roč.~539, DEC, s.~22-28. ISSN~0003-2697. Dostupné z: https://dx.doi.org/10.1016/j.ab.2017.09.018.

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