Rapid in situ toxicity testing with luminescent bacteria
Photorhabdus luminescens and Vibrio fischeri adapted to a small
portable luminometer

J 2017

Rapid in situ toxicity testing with luminescent bacteria Photorhabdus luminescens and Vibrio fischeri adapted to a small portable luminometer

MASNER, Petr, Barbora JAVŮRKOVÁ a Luděk BLÁHA

Základní údaje

Originální název

Rapid in situ toxicity testing with luminescent bacteria Photorhabdus luminescens and Vibrio fischeri adapted to a small portable luminometer

Autoři

MASNER, Petr (203 Česká republika, domácí), Barbora JAVŮRKOVÁ (203 Česká republika, domácí) a Luděk BLÁHA (203 Česká republika, garant, domácí)

Vydání

Environmental Science and Pollution Research, HEIDELBERG, SPRINGER HEIDELBERG, 2017, 0944-1344

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10511 Environmental sciences

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.800

Kód RIV

RIV/00216224:14310/17:00100220

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1007/s11356-016-8096-9

UT WoS

000397004600045

Klíčová slova anglicky

Photorhabdus luminescens; Vibrio fisheri; Whole effluent testing (WET); In situ testing; Portable biotest

Štítky

NZ, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 11. 4. 2018 11:06, Ing. Nicole Zrilić

Anotace

V originále

The present study demonstrates development of a rapid testing protocol based on a small portable luminometer using flash kinetic assessment of bacterial bioluminescence. The laboratory comparisons based on six model organic toxicants and two metals showed significant correlations between responses of freshwater bacteria Photorhabdus luminescens and standard marine bacterial species Vibrio fisheri. While P. luminescens was less sensitive in standard arrangements, the responses of both organisms were comparable in the newly introduced portable luminometer setup. The applicability and reproducibility of the portable luminometer protocol was further demonstrated in the assessment of 43 European wastewater effluents that were simultaneously tested for toxicity and analysed for 150 organic and 20 inorganic contaminants grouped into 13 major chemical classes. Clear association between the toxic responses in both compared bacterial species and the elevated levels of inorganic compounds (toxic metals), chlorophenols and benzotriazole anticorrosives was observed. The new protocol with a portable luminometer provides a fast (30 s) response and may be used as a tool for rapid in situ toxicity evaluation of freshwater environmental samples such as effluents.

Návaznosti

ED2.1.00/19.0382, projekt VaV

Název: CETOCOEN UPgrade

LO1214, projekt VaV

Název: Centrum pro výzkum toxických látek v prostředí (Akronym: RECETOX)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Centrum pro výzkum toxických látek v prostředí

Citovat

MASNER, Petr, Barbora JAVŮRKOVÁ a Luděk BLÁHA. Rapid in situ toxicity testing with luminescent bacteria Photorhabdus luminescens and Vibrio fischeri adapted to a small portable luminometer. Environmental Science and Pollution Research. HEIDELBERG: SPRINGER HEIDELBERG, 2017, roč. 24, č. 4, s. 3748-3758. ISSN 0944-1344. Dostupné z: https://dx.doi.org/10.1007/s11356-016-8096-9.

@article{1410107,
   author = {Masner, Petr and Javůrková, Barbora and Bláha, Luděk},
   article_location = {HEIDELBERG},
   article_number = {4},
   doi = {http://dx.doi.org/10.1007/s11356-016-8096-9},
   keywords = {Photorhabdus luminescens; Vibrio fisheri; Whole effluent testing (WET); In situ testing; Portable biotest},
   language = {eng},
   issn = {0944-1344},
   journal = {Environmental Science and Pollution Research},
   title = {Rapid in situ toxicity testing with luminescent bacteria Photorhabdus luminescens and Vibrio fischeri adapted to a small portable luminometer},
   url = {https://link.springer.com/article/10.1007%2Fs11356-016-8096-9},
   volume = {24},
   year = {2017}
}

TY  - JOUR
ID  - 1410107
AU  - Masner, Petr - Javůrková, Barbora - Bláha, Luděk
PY  - 2017
TI  - Rapid in situ toxicity testing with luminescent bacteria Photorhabdus luminescens and Vibrio fischeri adapted to a small portable luminometer
JF  - Environmental Science and Pollution Research
VL  - 24
IS  - 4
SP  - 3748-3758
EP  - 3748-3758
PB  - SPRINGER HEIDELBERG
SN  - 09441344
KW  - Photorhabdus luminescens
KW  - Vibrio fisheri
KW  - Whole effluent testing (WET)
KW  - In situ testing
KW  - Portable biotest
UR  - https://link.springer.com/article/10.1007%2Fs11356-016-8096-9
L2  - https://link.springer.com/article/10.1007%2Fs11356-016-8096-9
N2  - The present study demonstrates development of a rapid testing protocol based on a small portable luminometer using flash kinetic assessment of bacterial bioluminescence. The laboratory comparisons based on six model organic toxicants and two metals showed significant correlations between responses of freshwater bacteria Photorhabdus luminescens and standard marine bacterial species Vibrio fisheri. While P. luminescens was less sensitive in standard arrangements, the responses of both organisms were comparable in the newly introduced portable luminometer setup. The applicability and reproducibility of the portable luminometer protocol was further demonstrated in the assessment of 43 European wastewater effluents that were simultaneously tested for toxicity and analysed for 150 organic and 20 inorganic contaminants grouped into 13 major chemical classes. Clear association between the toxic responses in both compared bacterial species and the elevated levels of inorganic compounds (toxic metals), chlorophenols and benzotriazole anticorrosives was observed. The new protocol with a portable luminometer provides a fast (30 s) response and may be used as a tool for rapid in situ toxicity evaluation of freshwater environmental samples such as effluents.
ER  -

MASNER, Petr, Barbora JAVŮRKOVÁ a Luděk BLÁHA. Rapid in situ toxicity testing with luminescent bacteria Photorhabdus luminescens and Vibrio fischeri adapted to a small portable luminometer. \textit{Environmental Science and Pollution Research}. HEIDELBERG: SPRINGER HEIDELBERG, 2017, roč.~24, č.~4, s.~3748-3758. ISSN~0944-1344. Dostupné z: https://dx.doi.org/10.1007/s11356-016-8096-9.

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