Phytoestrogens and sterols in waters with cyanobacterial blooms
- Analytical methods and estrogenic potencies

J 2017

Phytoestrogens and sterols in waters with cyanobacterial blooms - Analytical methods and estrogenic potencies

PROCHÁZKOVÁ, Tereza, Eliška SYCHROVÁ, Barbora JAVŮRKOVÁ, Jaroslava VEČERKOVÁ, Jiří KOHOUTEK et. al.

Základní údaje

Originální název

Phytoestrogens and sterols in waters with cyanobacterial blooms - Analytical methods and estrogenic potencies

Autoři

PROCHÁZKOVÁ, Tereza (203 Česká republika, domácí), Eliška SYCHROVÁ (203 Česká republika, domácí), Barbora JAVŮRKOVÁ (203 Česká republika, domácí), Jaroslava VEČERKOVÁ (203 Česká republika, domácí), Jiří KOHOUTEK (203 Česká republika, domácí), Olga LEPSOVA-SKACELOVA (203 Česká republika), Luděk BLÁHA (203 Česká republika, domácí) a Klára HILSCHEROVÁ (203 Česká republika, garant, domácí)

Vydání

Chemosphere, Oxford, PERGAMON-ELSEVIER SCIENCE, 2017, 0045-6535

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10511 Environmental sciences

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.427

Kód RIV

RIV/00216224:14310/17:00100222

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.chemosphere.2016.12.006

UT WoS

000392897100013

Klíčová slova anglicky

Flavonoid; Sterol; Relative estrogenic potency; Estrogenicity; Reservoir

Štítky

NZ, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 10. 4. 2018 16:39, Ing. Nicole Zrilić

Anotace

V originále

Compounds with estrogenic potencies and their adverse effects in surface waters have received much attention. Both anthropogenic and natural compounds contribute to overall estrogenic activity in freshwaters. Recently, estrogenic potencies were also found to be associated with cyanobacteria and their blooms in surface waters. The present study developed and compared the solid phase extraction and LC-MS/MS analytical approaches for determination of phytoestrogens (8 flavonoids - biochanin A, coumestrol, daidzein, equol, formononetin, genistein, naringenin, apigenin and 5 sterols - ergosterol, (beta-sitosterol, stigmasterol, campesterol, brassicasterol) and cholesterol in water. The method was used for analyses of samples collected in stagnant water bodies dominated by different cyanobacterial species. Concentrations of individual flavonoids ranged from below the limit of detection to 3.58 ng/L. Sterols were present in higher amounts up to 2.25 mu g/L. Biological potencies of these phytoestrogens in vitro were characterized using the hER alpha-HeLa-9903 cell line. The relative estrogenic potencies (compared to model estrogen - 17 beta-estradiol) of flavonoids ranged from 2.25E-05 to 1.26E-03 with coumestrol being the most potent. None of the sterols elicited estrogenic response in the used bioassay. Estrogenic activity was detected in collected field water samples (maximum effect corresponding to 2.07 ng/L of 17 beta-estradiol equivalents, transcriptional assay). At maximum phytoestrogens accounted for only 1.56 pg/L of 17 beta-estradiol equivalents, contributing maximally 8.5% of the total estrogenicity of the water samples. Other compounds therefore, most likely of anthropogenic origin such as steroid estrogens, are probably the major drivers of total estrogenic effects in these surface waters.

Návaznosti

LM2015051, projekt VaV

Název: Centrum pro výzkum toxických látek v prostředí (Akronym: RECETOX RI)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Výzkumná infrastruktura RECETOX

LO1214, projekt VaV

Název: Centrum pro výzkum toxických látek v prostředí (Akronym: RECETOX)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Centrum pro výzkum toxických látek v prostředí

603437, interní kód MU

Název: SOLUTIONS - Solutions for present and future emerging pollutants in land and water resources management (Akronym: SOLUTIONS)

Investor: Evropská unie, SOLUTIONS - Solutions for present and future emerging pollutants in land and water resources management, Spolupráce

Citovat

PROCHÁZKOVÁ, Tereza, Eliška SYCHROVÁ, Barbora JAVŮRKOVÁ, Jaroslava VEČERKOVÁ, Jiří KOHOUTEK, Olga LEPSOVA-SKACELOVA, Luděk BLÁHA a Klára HILSCHEROVÁ. Phytoestrogens and sterols in waters with cyanobacterial blooms - Analytical methods and estrogenic potencies. Chemosphere. Oxford: PERGAMON-ELSEVIER SCIENCE, 2017, roč. 170, March, s. 104-112. ISSN 0045-6535. Dostupné z: https://dx.doi.org/10.1016/j.chemosphere.2016.12.006.

@article{1410111,
   author = {Procházková, Tereza and Sychrová, Eliška and Javůrková, Barbora and Večerková, Jaroslava and Kohoutek, Jiří and LepsovaandSkacelova, Olga and Bláha, Luděk and Hilscherová, Klára},
   article_location = {Oxford},
   article_number = {March},
   doi = {http://dx.doi.org/10.1016/j.chemosphere.2016.12.006},
   keywords = {Flavonoid; Sterol; Relative estrogenic potency; Estrogenicity; Reservoir},
   language = {eng},
   issn = {0045-6535},
   journal = {Chemosphere},
   title = {Phytoestrogens and sterols in waters with cyanobacterial blooms - Analytical methods and estrogenic potencies},
   url = {https://www.sciencedirect.com/science/article/pii/S0045653516317179?via%3Dihub},
   volume = {170},
   year = {2017}
}

TY  - JOUR
ID  - 1410111
AU  - Procházková, Tereza - Sychrová, Eliška - Javůrková, Barbora - Večerková, Jaroslava - Kohoutek, Jiří - Lepsova-Skacelova, Olga - Bláha, Luděk - Hilscherová, Klára
PY  - 2017
TI  - Phytoestrogens and sterols in waters with cyanobacterial blooms - Analytical methods and estrogenic potencies
JF  - Chemosphere
VL  - 170
IS  - March
SP  - 104-112
EP  - 104-112
PB  - PERGAMON-ELSEVIER SCIENCE
SN  - 00456535
KW  - Flavonoid
KW  - Sterol
KW  - Relative estrogenic potency
KW  - Estrogenicity
KW  - Reservoir
UR  - https://www.sciencedirect.com/science/article/pii/S0045653516317179?via%3Dihub
L2  - https://www.sciencedirect.com/science/article/pii/S0045653516317179?via%3Dihub
N2  - Compounds with estrogenic potencies and their adverse effects in surface waters have received much attention. Both anthropogenic and natural compounds contribute to overall estrogenic activity in freshwaters. Recently, estrogenic potencies were also found to be associated with cyanobacteria and their blooms in surface waters. The present study developed and compared the solid phase extraction and LC-MS/MS analytical approaches for determination of phytoestrogens (8 flavonoids - biochanin A, coumestrol, daidzein, equol, formononetin, genistein, naringenin, apigenin and 5 sterols - ergosterol, (beta-sitosterol, stigmasterol, campesterol, brassicasterol) and cholesterol in water. The method was used for analyses of samples collected in stagnant water bodies dominated by different cyanobacterial species. Concentrations of individual flavonoids ranged from below the limit of detection to 3.58 ng/L. Sterols were present in higher amounts up to 2.25 mu g/L. Biological potencies of these phytoestrogens in vitro were characterized using the hER alpha-HeLa-9903 cell line. The relative estrogenic potencies (compared to model estrogen - 17 beta-estradiol) of flavonoids ranged from 2.25E-05 to 1.26E-03 with coumestrol being the most potent. None of the sterols elicited estrogenic response in the used bioassay. Estrogenic activity was detected in collected field water samples (maximum effect corresponding to 2.07 ng/L of 17 beta-estradiol equivalents, transcriptional assay). At maximum phytoestrogens accounted for only 1.56 pg/L of 17 beta-estradiol equivalents, contributing maximally 8.5% of the total estrogenicity of the water samples. Other compounds therefore, most likely of anthropogenic origin such as steroid estrogens, are probably the major drivers of total estrogenic effects in these surface waters.
ER  -

PROCHÁZKOVÁ, Tereza, Eliška SYCHROVÁ, Barbora JAVŮRKOVÁ, Jaroslava VEČERKOVÁ, Jiří KOHOUTEK, Olga LEPSOVA-SKACELOVA, Luděk BLÁHA a Klára HILSCHEROVÁ. Phytoestrogens and sterols in waters with cyanobacterial blooms - Analytical methods and estrogenic potencies. \textit{Chemosphere}. Oxford: PERGAMON-ELSEVIER SCIENCE, 2017, roč.~170, March, s.~104-112. ISSN~0045-6535. Dostupné z: https://dx.doi.org/10.1016/j.chemosphere.2016.12.006.

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