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@article{1410111, author = {Procházková, Tereza and Sychrová, Eliška and Javůrková, Barbora and Večerková, Jaroslava and Kohoutek, Jiří and LepsovaandSkacelova, Olga and Bláha, Luděk and Hilscherová, Klára}, article_location = {Oxford}, article_number = {March}, doi = {http://dx.doi.org/10.1016/j.chemosphere.2016.12.006}, keywords = {Flavonoid; Sterol; Relative estrogenic potency; Estrogenicity; Reservoir}, language = {eng}, issn = {0045-6535}, journal = {Chemosphere}, title = {Phytoestrogens and sterols in waters with cyanobacterial blooms - Analytical methods and estrogenic potencies}, url = {https://www.sciencedirect.com/science/article/pii/S0045653516317179?via%3Dihub}, volume = {170}, year = {2017} }
TY - JOUR ID - 1410111 AU - Procházková, Tereza - Sychrová, Eliška - Javůrková, Barbora - Večerková, Jaroslava - Kohoutek, Jiří - Lepsova-Skacelova, Olga - Bláha, Luděk - Hilscherová, Klára PY - 2017 TI - Phytoestrogens and sterols in waters with cyanobacterial blooms - Analytical methods and estrogenic potencies JF - Chemosphere VL - 170 IS - March SP - 104-112 EP - 104-112 PB - PERGAMON-ELSEVIER SCIENCE SN - 00456535 KW - Flavonoid KW - Sterol KW - Relative estrogenic potency KW - Estrogenicity KW - Reservoir UR - https://www.sciencedirect.com/science/article/pii/S0045653516317179?via%3Dihub L2 - https://www.sciencedirect.com/science/article/pii/S0045653516317179?via%3Dihub N2 - Compounds with estrogenic potencies and their adverse effects in surface waters have received much attention. Both anthropogenic and natural compounds contribute to overall estrogenic activity in freshwaters. Recently, estrogenic potencies were also found to be associated with cyanobacteria and their blooms in surface waters. The present study developed and compared the solid phase extraction and LC-MS/MS analytical approaches for determination of phytoestrogens (8 flavonoids - biochanin A, coumestrol, daidzein, equol, formononetin, genistein, naringenin, apigenin and 5 sterols - ergosterol, (beta-sitosterol, stigmasterol, campesterol, brassicasterol) and cholesterol in water. The method was used for analyses of samples collected in stagnant water bodies dominated by different cyanobacterial species. Concentrations of individual flavonoids ranged from below the limit of detection to 3.58 ng/L. Sterols were present in higher amounts up to 2.25 mu g/L. Biological potencies of these phytoestrogens in vitro were characterized using the hER alpha-HeLa-9903 cell line. The relative estrogenic potencies (compared to model estrogen - 17 beta-estradiol) of flavonoids ranged from 2.25E-05 to 1.26E-03 with coumestrol being the most potent. None of the sterols elicited estrogenic response in the used bioassay. Estrogenic activity was detected in collected field water samples (maximum effect corresponding to 2.07 ng/L of 17 beta-estradiol equivalents, transcriptional assay). At maximum phytoestrogens accounted for only 1.56 pg/L of 17 beta-estradiol equivalents, contributing maximally 8.5% of the total estrogenicity of the water samples. Other compounds therefore, most likely of anthropogenic origin such as steroid estrogens, are probably the major drivers of total estrogenic effects in these surface waters. ER -
PROCHÁZKOVÁ, Tereza, Eliška SYCHROVÁ, Barbora JAVŮRKOVÁ, Jaroslava VEČERKOVÁ, Jiří KOHOUTEK, Olga LEPSOVA-SKACELOVA, Luděk BLÁHA a Klára HILSCHEROVÁ. Phytoestrogens and sterols in waters with cyanobacterial blooms - Analytical methods and estrogenic potencies. \textit{Chemosphere}. Oxford: PERGAMON-ELSEVIER SCIENCE, 2017, roč.~170, March, s.~104-112. ISSN~0045-6535. Dostupné z: https://dx.doi.org/10.1016/j.chemosphere.2016.12.006.
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