DVOŘÁČKOVÁ, Martina, B. RAPOSO, Petr MATULA, J. FUCHS, V. SCHUBERT, Vratislav PEŠKA, B. DESVOYES, C. GUTIERREZ and Jiří FAJKUS. Replication of ribosomal DNA in Arabidopsis occurs both inside and outside the nucleolus during S phase progression. Journal of Cell Science, Cambridge: Company of Biologists Ltd., 2018, vol. 131, No 2, p. nestránkováno. ISSN 0021-9533. doi:10.1242/jcs.202416.
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Basic information
Original name Replication of ribosomal DNA in Arabidopsis occurs both inside and outside the nucleolus during S phase progression
Authors DVOŘÁČKOVÁ, Martina (203 Czech Republic, belonging to the institution), B. RAPOSO (724 Spain), Petr MATULA (203 Czech Republic, belonging to the institution), J. FUCHS (276 Germany), V. SCHUBERT (276 Germany), Vratislav PEŠKA (203 Czech Republic, belonging to the institution), B. DESVOYES (724 Spain), C. GUTIERREZ (724 Spain) and Jiří FAJKUS (203 Czech Republic, guarantor, belonging to the institution).
Edition Journal of Cell Science, Cambridge, Company of Biologists Ltd. 2018, 0021-9533.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10601 Cell biology
Country of publisher United Kingdom of Great Britain and Northern Ireland
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 4.517
RIV identification code RIV/00216224:14740/18:00100849
Organization unit Central European Institute of Technology
Doi http://dx.doi.org/10.1242/jcs.202416
UT WoS 000424842400004
Keywords in English A. thaliana rDNA; Replication; Nucleolus; Flow cytometry; Structured illumination microscopy
Tags cbia-web, rivok
Tags International impact, Reviewed
Changed by Changed by: Mgr. Pavla Foltynová, Ph.D., učo 106624. Changed: 19/3/2019 09:06.
Abstract
Ribosomal RNA genes (rDNA) have been used as valuable experimental systems in numerous studies. Here, we focus on elucidating the spatiotemporal organisation of rDNA replication in Arabidopsis thaliana. To determine the subnuclear distribution of rDNA and the progression of its replication during the S phase, we apply 5-ethynyl-2'-deoxyuridine (EdU) labelling, fluorescence-activated cell sorting, fluorescence in situ hybridization and structured illumination microscopy. We show that rDNA is replicated inside and outside the nucleolus, where active transcription occurs at the same time. Nascent rDNA shows a maximum of nucleolar associations during early S phase. In addition to EdU patterns typical for early or late S phase, we describe two intermediate EdU profiles characteristic for mid S phase. Moreover, the use of lines containing mutations in the chromatin assembly factor-1 gene fas1 and wild-type progeny of fas1xfas2 crosses depleted of inactive copies allows for selective observation of the replication pattern of active rDNA. High-resolution data are presented, revealing the culmination of replication in the mid S phase in the nucleolus and its vicinity. Taken together, our results provide a detailed snapshot of replication of active and inactive rDNA during S phase progression.
Links
GJ16-04166Y, research and development projectName: Chromatin asociované faktory podílející se na regulaci 45SrDNA v Arabidopsis
LH15189, research and development projectName: Struktura a dynamika 45S rDNA lokusů v Arabidopsis thaliana
Investor: Ministry of Education, Youth and Sports of the CR, KONTAKT II
LM2015062, research and development projectName: Národní infrastruktura pro biologické a medicínské zobrazování
Investor: Ministry of Education, Youth and Sports of the CR, Large Infrastructures for Research, Development and Innovation
LQ1601, research and development projectName: CEITEC 2020 (Acronym: CEITEC2020)
Investor: Ministry of Education, Youth and Sports of the CR, National Feasibility Programme II
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