VEČEŘA, Josef, Jana KUDOVÁ, Jan KUČERA, Lukáš KUBALA a Jiří PACHERNÍK. Neural Differentiation Is Inhibited through HIF1 alpha/ beta-Catenin Signaling in Embryoid Bodies. Stem Cells International. London: HINDAWI LTD, 2017, Neuveden, prosinec, s. nestránkováno, 12 s. ISSN 1687-966X. Dostupné z: https://dx.doi.org/10.1155/2017/8715798. |
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@article{1410440, author = {Večeřa, Josef and Kudová, Jana and Kučera, Jan and Kubala, Lukáš and Pacherník, Jiří}, article_location = {London}, article_number = {prosinec}, doi = {http://dx.doi.org/10.1155/2017/8715798}, keywords = {STEM-CELL FATE; HYPOXIA; OXYGEN; HIF; CATENIN}, language = {eng}, issn = {1687-966X}, journal = {Stem Cells International}, title = {Neural Differentiation Is Inhibited through HIF1 alpha/ beta-Catenin Signaling in Embryoid Bodies}, url = {http://apps.webofknowledge.com/full_record.do?product=WOS&search_mode=GeneralSearch&qid=1&SID=E5gWduQPo6sNAt8bJHA&page=1&doc=4}, volume = {Neuveden}, year = {2017} }
TY - JOUR ID - 1410440 AU - Večeřa, Josef - Kudová, Jana - Kučera, Jan - Kubala, Lukáš - Pacherník, Jiří PY - 2017 TI - Neural Differentiation Is Inhibited through HIF1 alpha/ beta-Catenin Signaling in Embryoid Bodies JF - Stem Cells International VL - Neuveden IS - prosinec SP - nestránkováno EP - nestránkováno PB - HINDAWI LTD SN - 1687966X KW - STEM-CELL FATE KW - HYPOXIA KW - OXYGEN KW - HIF KW - CATENIN UR - http://apps.webofknowledge.com/full_record.do?product=WOS&search_mode=GeneralSearch&qid=1&SID=E5gWduQPo6sNAt8bJHA&page=1&doc=4 N2 - Extensive research in the field of stem cells and developmental biology has revealed evidence of the role of hypoxia as an important factor regulating self-renewal and differentiation. However, comprehensive information about the exact hypoxia-mediated regulatory mechanism of stem cell fate during early embryonic development is still missing. Using a model of embryoid bodies (EBs) derived from murine embryonic stem cells (ESC), we here tried to encrypt the role of hypoxia-inducible factor 1 alpha (HIF1 alpha) in neural fate during spontaneous differentiation. EBs derived from ESC with the ablated gene for HIF1a had abnormally increased neuronal characteristics during differentiation. An increased neural phenotype in Hif1 alpha(-/-) EBs was accompanied by the disruption of beta-catenin signaling together with the increased cytoplasmic degradation of beta-catenin. The knock-in of Hif1 alpha, as well as beta-catenin ectopic overexpression in Hif1 alpha(-/-) EBs, induced a reduction in neural markers to the levels observed in wild-type EBs. Interestingly, direct interaction between HIF1 alpha and beta-catenin was demonstrated by immunoprecipitation analysis of the nuclear fraction of wild-type EBs. Together, these results emphasize the regulatory role of HIF1 alpha in beta-catenin stabilization during spontaneous differentiation, which seems to be a crucial mechanism for the natural inhibition of premature neural differentiation. ER -
VEČEŘA, Josef, Jana KUDOVÁ, Jan KUČERA, Lukáš KUBALA a Jiří PACHERNÍK. Neural Differentiation Is Inhibited through HIF1 alpha/ beta-Catenin Signaling in Embryoid Bodies. \textit{Stem Cells International}. London: HINDAWI LTD, 2017, Neuveden, prosinec, s.~nestránkováno, 12 s. ISSN~1687-966X. Dostupné z: https://dx.doi.org/10.1155/2017/8715798.
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