Protection of Arabidopsis Blunt-Ended Telomeres Is Mediated by
a Physical Association with the Ku Heterodimer

J 2017

Protection of Arabidopsis Blunt-Ended Telomeres Is Mediated by a Physical Association with the Ku Heterodimer

VALUCHOVÁ, Soňa, Jaroslav FULNEČEK, Zbyněk PROKOP, Peggy STOLT-BERGNER, Eliška JANOUŠKOVÁ et. al.

Základní údaje

Originální název

Protection of Arabidopsis Blunt-Ended Telomeres Is Mediated by a Physical Association with the Ku Heterodimer

Autoři

VALUCHOVÁ, Soňa (703 Slovensko, domácí), Jaroslav FULNEČEK (203 Česká republika, domácí), Zbyněk PROKOP (203 Česká republika, domácí), Peggy STOLT-BERGNER (40 Rakousko), Eliška JANOUŠKOVÁ (203 Česká republika, domácí), Ctirad HOFR (203 Česká republika, domácí) a Karel ŘÍHA (203 Česká republika, garant, domácí)

Vydání

Plant Cell, ROCKVILLE, AMER SOC PLANT BIOLOGISTS, 2017, 1040-4651

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10611 Plant sciences, botany

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 8.228

Kód RIV

RIV/00216224:14740/17:00095562

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1105/tpc.17.00064

UT WoS

000404989600026

Klíčová slova anglicky

STRAND BREAK REPAIR; INDUCED FLUORESCENCE ENHANCEMENT; SINGLE DNA END; MAMMALIAN TELOMERES; PROTEIN; LENGTH; COMPLEX; DAMAGE; FUSIONS; YEAST

Štítky

OA, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 15. 3. 2018 10:49, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

Telomeres form specialized chromatin that protects natural chromosome termini from being recognized as DNA double-strand breaks. Plants possess unusual blunt-ended telomeres that are unable to form t-loops or complex with single-strand DNA binding proteins, raising the question of the mechanism behind their protection. We have previously suggested that blunt-ended telomeres in Arabidopsis thaliana are protected by Ku, a DNA repair factor with a high affinity for DNA ends. In nonhomologous end joining, Ku loads onto broken DNA via a channel consisting of positively charged amino acids. Here, we demonstrate that while association of Ku with plant telomeres also depends on this channel, Ku's requirements for DNA binding differ between DNA repair and telomere protection. We show that a Ku complex proficient in DNA loading but impaired in translocation along DNA is able to protect blunt-ended telomeres but is deficient in DNA repair. This suggests that Ku physically sequesters blunt-ended telomeres within its DNA binding channel, shielding them from other DNA repair machineries.

Návaznosti

GAP205/12/0550, projekt VaV

Název: Dynamika vzniku shelterinového komplexu

Investor: Grantová agentura ČR, Dynamika vzniku shelterinového komplexu

LM2015051, projekt VaV

Název: Centrum pro výzkum toxických látek v prostředí (Akronym: RECETOX RI)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Výzkumná infrastruktura RECETOX

LO1214, projekt VaV

Název: Centrum pro výzkum toxických látek v prostředí (Akronym: RECETOX)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Centrum pro výzkum toxických látek v prostředí

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

1304130933, interní kód MU

Název: Blunt-ended telomeres: dissecting the novel mechanism of chromosome end protection

Investor: EMBO (European Molecular Biology Organization), Blunt-ended telomeres: dissecting the novel mechanism of chromosome end protection

Citovat

VALUCHOVÁ, Soňa, Jaroslav FULNEČEK, Zbyněk PROKOP, Peggy STOLT-BERGNER, Eliška JANOUŠKOVÁ, Ctirad HOFR a Karel ŘÍHA. Protection of Arabidopsis Blunt-Ended Telomeres Is Mediated by a Physical Association with the Ku Heterodimer. Plant Cell. ROCKVILLE: AMER SOC PLANT BIOLOGISTS, 2017, roč. 29, č. 6, s. 1533-1545. ISSN 1040-4651. Dostupné z: https://dx.doi.org/10.1105/tpc.17.00064.

@article{1410576,
   author = {Valuchová, Soňa and Fulneček, Jaroslav and Prokop, Zbyněk and StoltandBergner, Peggy and Janoušková, Eliška and Hofr, Ctirad and Říha, Karel},
   article_location = {ROCKVILLE},
   article_number = {6},
   doi = {http://dx.doi.org/10.1105/tpc.17.00064},
   keywords = {STRAND BREAK REPAIR; INDUCED FLUORESCENCE ENHANCEMENT; SINGLE DNA END; MAMMALIAN TELOMERES; PROTEIN; LENGTH; COMPLEX; DAMAGE; FUSIONS; YEAST},
   language = {eng},
   issn = {1040-4651},
   journal = {Plant Cell},
   title = {Protection of Arabidopsis Blunt-Ended Telomeres Is Mediated by a Physical Association with the Ku Heterodimer},
   url = {http://www.plantcell.org/content/29/6/1533},
   volume = {29},
   year = {2017}
}

TY  - JOUR
ID  - 1410576
AU  - Valuchová, Soňa - Fulneček, Jaroslav - Prokop, Zbyněk - Stolt-Bergner, Peggy - Janoušková, Eliška - Hofr, Ctirad - Říha, Karel
PY  - 2017
TI  - Protection of Arabidopsis Blunt-Ended Telomeres Is Mediated by a Physical Association with the Ku Heterodimer
JF  - Plant Cell
VL  - 29
IS  - 6
SP  - 1533-1545
EP  - 1533-1545
PB  - AMER SOC PLANT BIOLOGISTS
SN  - 10404651
KW  - STRAND BREAK REPAIR
KW  - INDUCED FLUORESCENCE ENHANCEMENT
KW  - SINGLE DNA END
KW  - MAMMALIAN TELOMERES
KW  - PROTEIN
KW  - LENGTH
KW  - COMPLEX
KW  - DAMAGE
KW  - FUSIONS
KW  - YEAST
UR  - http://www.plantcell.org/content/29/6/1533
L2  - http://www.plantcell.org/content/29/6/1533
N2  - Telomeres form specialized chromatin that protects natural chromosome termini from being recognized as DNA double-strand breaks. Plants possess unusual blunt-ended telomeres that are unable to form t-loops or complex with single-strand DNA binding proteins, raising the question of the mechanism behind their protection. We have previously suggested that blunt-ended telomeres in Arabidopsis thaliana are protected by Ku, a DNA repair factor with a high affinity for DNA ends. In nonhomologous end joining, Ku loads onto broken DNA via a channel consisting of positively charged amino acids. Here, we demonstrate that while association of Ku with plant telomeres also depends on this channel, Ku's requirements for DNA binding differ between DNA repair and telomere protection. We show that a Ku complex proficient in DNA loading but impaired in translocation along DNA is able to protect blunt-ended telomeres but is deficient in DNA repair. This suggests that Ku physically sequesters blunt-ended telomeres within its DNA binding channel, shielding them from other DNA repair machineries.
ER  -

VALUCHOVÁ, Soňa, Jaroslav FULNEČEK, Zbyněk PROKOP, Peggy STOLT-BERGNER, Eliška JANOUŠKOVÁ, Ctirad HOFR a Karel ŘÍHA. Protection of Arabidopsis Blunt-Ended Telomeres Is Mediated by a Physical Association with the Ku Heterodimer. \textit{Plant Cell}. ROCKVILLE: AMER SOC PLANT BIOLOGISTS, 2017, roč.~29, č.~6, s.~1533-1545. ISSN~1040-4651. Dostupné z: https://dx.doi.org/10.1105/tpc.17.00064.

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