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@article{1410576,
author = {Valuchová, Soňa and Fulneček, Jaroslav and Prokop, Zbyněk and StoltandBergner, Peggy and Janoušková, Eliška and Hofr, Ctirad and Říha, Karel},
article_location = {ROCKVILLE},
article_number = {6},
doi = {http://dx.doi.org/10.1105/tpc.17.00064},
keywords = {STRAND BREAK REPAIR; INDUCED FLUORESCENCE ENHANCEMENT; SINGLE DNA END; MAMMALIAN TELOMERES; PROTEIN; LENGTH; COMPLEX; DAMAGE; FUSIONS; YEAST},
language = {eng},
issn = {1040-4651},
journal = {Plant Cell},
title = {Protection of Arabidopsis Blunt-Ended Telomeres Is Mediated by a Physical Association with the Ku Heterodimer},
url = {http://www.plantcell.org/content/29/6/1533},
volume = {29},
year = {2017}
}
TY - JOUR
ID - 1410576
AU - Valuchová, Soňa - Fulneček, Jaroslav - Prokop, Zbyněk - Stolt-Bergner, Peggy - Janoušková, Eliška - Hofr, Ctirad - Říha, Karel
PY - 2017
TI - Protection of Arabidopsis Blunt-Ended Telomeres Is Mediated by a Physical Association with the Ku Heterodimer
JF - Plant Cell
VL - 29
IS - 6
SP - 1533-1545
EP - 1533-1545
PB - AMER SOC PLANT BIOLOGISTS
SN - 10404651
KW - STRAND BREAK REPAIR
KW - INDUCED FLUORESCENCE ENHANCEMENT
KW - SINGLE DNA END
KW - MAMMALIAN TELOMERES
KW - PROTEIN
KW - LENGTH
KW - COMPLEX
KW - DAMAGE
KW - FUSIONS
KW - YEAST
UR - http://www.plantcell.org/content/29/6/1533
L2 - http://www.plantcell.org/content/29/6/1533
N2 - Telomeres form specialized chromatin that protects natural chromosome termini from being recognized as DNA double-strand breaks. Plants possess unusual blunt-ended telomeres that are unable to form t-loops or complex with single-strand DNA binding proteins, raising the question of the mechanism behind their protection. We have previously suggested that blunt-ended telomeres in Arabidopsis thaliana are protected by Ku, a DNA repair factor with a high affinity for DNA ends. In nonhomologous end joining, Ku loads onto broken DNA via a channel consisting of positively charged amino acids. Here, we demonstrate that while association of Ku with plant telomeres also depends on this channel, Ku's requirements for DNA binding differ between DNA repair and telomere protection. We show that a Ku complex proficient in DNA loading but impaired in translocation along DNA is able to protect blunt-ended telomeres but is deficient in DNA repair. This suggests that Ku physically sequesters blunt-ended telomeres within its DNA binding channel, shielding them from other DNA repair machineries.
ER -
VALUCHOVÁ, Soňa, Jaroslav FULNEČEK, Zbyněk PROKOP, Peggy STOLT-BERGNER, Eliška JANOUŠKOVÁ, Ctirad HOFR a Karel ŘÍHA. Protection of Arabidopsis Blunt-Ended Telomeres Is Mediated by a Physical Association with the Ku Heterodimer. \textit{Plant Cell}. ROCKVILLE: AMER SOC PLANT BIOLOGISTS, 2017, roč.~29, č.~6, s.~1533-1545. ISSN~1040-4651. Dostupné z: https://dx.doi.org/10.1105/tpc.17.00064.
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