Elasticity and tumorigenic characteristics of cells in a
monolayer after nanosecond pulsed electric field exposure

J 2017

Elasticity and tumorigenic characteristics of cells in a monolayer after nanosecond pulsed electric field exposure

STEUER, A., K. WENDE, Pavel BABICA a J. F. KOLB

Základní údaje

Originální název

Elasticity and tumorigenic characteristics of cells in a monolayer after nanosecond pulsed electric field exposure

Autoři

STEUER, A. (276 Německo), K. WENDE (276 Německo), Pavel BABICA (203 Česká republika, garant, domácí) a J. F. KOLB (276 Německo)

Vydání

European Biophysics Journal With Biophysics Letters, NEW YORK, SPRINGER, 2017, 0175-7571

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10610 Biophysics

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 1.935

Kód RIV

RIV/00216224:14310/17:00100310

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1007/s00249-017-1205-y

UT WoS

000407241700008

Klíčová slova anglicky

nsPEF; Elasticity; Elastic modulus; AFM; Actin cytoskeleton; Anchorage-independent growth

Štítky

NZ, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 3. 4. 2018 13:47, Ing. Nicole Zrilić

Anotace

V originále

Nanosecond pulsed electric fields (nsPEFs) applied to cells can induce different biological effects depending on pulse duration and field strength. One known process is the induction of apoptosis whereby nsPEFs are currently investigated as a novel cancer therapy. Another and probably related change is the breakdown of the cytoskeleton. We investigated the elasticity of rat liver epithelial cells WB-F344 in a monolayer using atomic force microscopy (AFM) with respect to the potential of cells to undergo malignant transformation or to develop a potential to metastasize. We found that the elastic modulus of the cells decreased significantly within the first 8 min after treatment with 20 pulses of 100 ns and with a field strength of 20 kV/cm but was still higher than the elasticity of their tumorigenic counterpart WB-ras. AFM measurements and immunofluorescent staining showed that the cellular actin cytoskeleton became reorganized within 5 min. However, both a colony formation assay and a cell migration assay revealed no significant changes after nsPEF treatment, implying that cells seem not to adopt malignant characteristics associated with metastasis formation despite the induced transient changes to elasticity and cytoskeleton that can be observed for up to 1 h.

Návaznosti

LM2015051, projekt VaV

Název: Centrum pro výzkum toxických látek v prostředí (Akronym: RECETOX RI)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Výzkumná infrastruktura RECETOX

LO1214, projekt VaV

Název: Centrum pro výzkum toxických látek v prostředí (Akronym: RECETOX)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Centrum pro výzkum toxických látek v prostředí

Citovat

STEUER, A., K. WENDE, Pavel BABICA a J. F. KOLB. Elasticity and tumorigenic characteristics of cells in a monolayer after nanosecond pulsed electric field exposure. European Biophysics Journal With Biophysics Letters. NEW YORK: SPRINGER, 2017, roč. 46, č. 6, s. 567-580. ISSN 0175-7571. Dostupné z: https://dx.doi.org/10.1007/s00249-017-1205-y.

@article{1410911,
   author = {Steuer, A. and Wende, K. and Babica, Pavel and Kolb, J. F.},
   article_location = {NEW YORK},
   article_number = {6},
   doi = {http://dx.doi.org/10.1007/s00249-017-1205-y},
   keywords = {nsPEF; Elasticity; Elastic modulus; AFM; Actin cytoskeleton; Anchorage-independent growth},
   language = {eng},
   issn = {0175-7571},
   journal = {European Biophysics Journal With Biophysics Letters},
   title = {Elasticity and tumorigenic characteristics of cells in a monolayer after nanosecond pulsed electric field exposure},
   url = {https://link.springer.com/article/10.1007%2Fs00249-017-1205-y},
   volume = {46},
   year = {2017}
}

TY  - JOUR
ID  - 1410911
AU  - Steuer, A. - Wende, K. - Babica, Pavel - Kolb, J. F.
PY  - 2017
TI  - Elasticity and tumorigenic characteristics of cells in a monolayer after nanosecond pulsed electric field exposure
JF  - European Biophysics Journal With Biophysics Letters
VL  - 46
IS  - 6
SP  - 567-580
EP  - 567-580
PB  - SPRINGER
SN  - 01757571
KW  - nsPEF
KW  - Elasticity
KW  - Elastic modulus
KW  - AFM
KW  - Actin cytoskeleton
KW  - Anchorage-independent growth
UR  - https://link.springer.com/article/10.1007%2Fs00249-017-1205-y
L2  - https://link.springer.com/article/10.1007%2Fs00249-017-1205-y
N2  - Nanosecond pulsed electric fields (nsPEFs) applied to cells can induce different biological effects depending on pulse duration and field strength. One known process is the induction of apoptosis whereby nsPEFs are currently investigated as a novel cancer therapy. Another and probably related change is the breakdown of the cytoskeleton. We investigated the elasticity of rat liver epithelial cells WB-F344 in a monolayer using atomic force microscopy (AFM) with respect to the potential of cells to undergo malignant transformation or to develop a potential to metastasize. We found that the elastic modulus of the cells decreased significantly within the first 8 min after treatment with 20 pulses of 100 ns and with a field strength of 20 kV/cm but was still higher than the elasticity of their tumorigenic counterpart WB-ras. AFM measurements and immunofluorescent staining showed that the cellular actin cytoskeleton became reorganized within 5 min. However, both a colony formation assay and a cell migration assay revealed no significant changes after nsPEF treatment, implying that cells seem not to adopt malignant characteristics associated with metastasis formation despite the induced transient changes to elasticity and cytoskeleton that can be observed for up to 1 h.
ER  -

STEUER, A., K. WENDE, Pavel BABICA a J. F. KOLB. Elasticity and tumorigenic characteristics of cells in a monolayer after nanosecond pulsed electric field exposure. \textit{European Biophysics Journal With Biophysics Letters}. NEW YORK: SPRINGER, 2017, roč.~46, č.~6, s.~567-580. ISSN~0175-7571. Dostupné z: https://dx.doi.org/10.1007/s00249-017-1205-y.

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