Chlorination and ozonation differentially reduced the
microcystin content and tumour promoting activity of a complex
cyanobacterial extract

J 2017

Chlorination and ozonation differentially reduced the microcystin content and tumour promoting activity of a complex cyanobacterial extract

SOVADINOVÁ, Iva, Pavel BABICA, Ondřej ADAMOVSKÝ, Alla ALPATOVA, Volodymyr TARAMARA et. al.

Základní údaje

Originální název

Chlorination and ozonation differentially reduced the microcystin content and tumour promoting activity of a complex cyanobacterial extract

Autoři

SOVADINOVÁ, Iva (203 Česká republika, garant, domácí), Pavel BABICA (203 Česká republika, domácí), Ondřej ADAMOVSKÝ (203 Česká republika, domácí), Alla ALPATOVA (124 Kanada), Volodymyr TARAMARA (840 Spojené státy), Brad L. UPHAM (840 Spojené státy) a Luděk BLÁHA (203 Česká republika, domácí)

Vydání

Advances in Oceanography and Limnology, 2017, 1947-573X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10502 Oceanography

Stát vydavatele

Itálie

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Kód RIV

RIV/00216224:14310/17:00095572

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.4081/aiol.2017.6342

Klíčová slova anglicky

Cyanobacteria; water treatment; toxicity; chlorination; ozonation; microcystin

Štítky

NZ, rivok

Změněno: 31. 5. 2022 14:31, RNDr. Pavel Šmerk, Ph.D.

Anotace

V originále

Despite intensive research and management efforts in the past decades, cyanobacterial blooms and their toxins, such as microcystins (MCs), continue to represent a major ecological and health problem in fresh waters throughout the world. Our objective was to compare the efficacy of two commonly used drinking water treatment technologies, chlorination and ozonation, in removing MCs and in reducing tumour promotion-related effects of cyanobacteria, such as inhibition of gap junctional intercellular communication (GJIC) and activation of mitogen activated protein kinases (MAPKs) in a rat liver epithelial stem-like cell line (WB-F344). This combined chemical and bioassay approach demonstrated that ozone effectively removed all MCs from an extract of a globally important bloom-forming cyanobacterium, Microcystis sp. Ozone also significantly reduced the overall tumour promotional potency of the cyanobacterial extract, as indicated by a substantial reduction in the ability of the extract to inhibit GJIC and activate extracellular receptor kinase 1/2 (ERK1/2). Although comparable reduction of total organic carbon was achieved by ozone and chlorine treatment, chlorination was much less effective in removing MCs and reducing the effects on GJIC. Chlorination had a biphasic effect with an observed decrease of extract-induced activation of ERK1/2 at the lower chlorine doses; whereas at high doses of chlorine the by-products of chlorination actually induced the activation of ERK1/2. The extracts induced p38 activation, and chlorination was not effective in reversing this effect, while ozone did reverse this effect, albeit not as much as the activation of ERK1/2. Thus, ozone was effective in reducing the toxicity of cyanobacterial extracts while chlorination was not only lacking efficacy, but at high doses of chlorination further produced by-products that were equally toxic as the untreated samples. Our study indicates the value of using an effect-based approach to assess the efficacy of water treatment systems in removing toxins, and more specifically demonstrates that ozone was more effective at reducing the toxic potential of cyanobacterial-contaminated water.

Návaznosti

GA15-12408S, projekt VaV

Název: Role kmenových a diferencovaných jaterních buněk v hepatotoxicitě a hepatokarcinogenitě indukované cyanotoxiny

Investor: Grantová agentura ČR, Role kmenových a diferencovaných jaterních buněk v hepatotoxicitě a hepatokarcinogenitě indukované cyanotoxiny

LM2015051, projekt VaV

Název: Centrum pro výzkum toxických látek v prostředí (Akronym: RECETOX RI)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Výzkumná infrastruktura RECETOX

LO1214, projekt VaV

Název: Centrum pro výzkum toxických látek v prostředí (Akronym: RECETOX)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Centrum pro výzkum toxických látek v prostředí

Citovat

SOVADINOVÁ, Iva, Pavel BABICA, Ondřej ADAMOVSKÝ, Alla ALPATOVA, Volodymyr TARAMARA, Brad L. UPHAM a Luděk BLÁHA. Chlorination and ozonation differentially reduced the microcystin content and tumour promoting activity of a complex cyanobacterial extract. Advances in Oceanography and Limnology. 2017, roč. 8, č. 1, s. 107-120. ISSN 1947-573X. Dostupné z: https://dx.doi.org/10.4081/aiol.2017.6342.

@article{1411095,
   author = {Sovadinová, Iva and Babica, Pavel and Adamovský, Ondřej and Alpatova, Alla and Taramara, Volodymyr and Upham, Brad L. and Bláha, Luděk},
   article_number = {1},
   doi = {http://dx.doi.org/10.4081/aiol.2017.6342},
   keywords = {Cyanobacteria; water treatment; toxicity; chlorination; ozonation; microcystin},
   language = {eng},
   issn = {1947-573X},
   journal = {Advances in Oceanography and Limnology},
   title = {Chlorination and ozonation differentially reduced the microcystin content and tumour promoting activity of a complex cyanobacterial extract},
   url = {http://pagepressjournals.org/index.php/aiol/article/view/6342},
   volume = {8},
   year = {2017}
}

TY  - JOUR
ID  - 1411095
AU  - Sovadinová, Iva - Babica, Pavel - Adamovský, Ondřej - Alpatova, Alla - Taramara, Volodymyr - Upham, Brad L. - Bláha, Luděk
PY  - 2017
TI  - Chlorination and ozonation differentially reduced the microcystin content and tumour promoting activity of a complex cyanobacterial extract
JF  - Advances in Oceanography and Limnology
VL  - 8
IS  - 1
SP  - 107-120
EP  - 107-120
SN  - 1947573X
KW  - Cyanobacteria
KW  - water treatment
KW  - toxicity
KW  - chlorination
KW  - ozonation
KW  - microcystin
UR  - http://pagepressjournals.org/index.php/aiol/article/view/6342
L2  - http://pagepressjournals.org/index.php/aiol/article/view/6342
N2  - Despite intensive research and management efforts in the past decades, cyanobacterial blooms and their toxins, such as microcystins (MCs), continue to represent a major ecological and health problem in fresh waters throughout the world. Our objective was to compare the efficacy of two commonly used drinking water treatment technologies, chlorination and ozonation, in removing MCs and in reducing tumour promotion-related effects of cyanobacteria, such as inhibition of gap junctional intercellular communication (GJIC) and activation of mitogen activated protein kinases (MAPKs) in a rat liver epithelial stem-like cell line (WB-F344). This combined chemical and bioassay approach demonstrated that ozone effectively removed all MCs from an extract of a globally important bloom-forming cyanobacterium, Microcystis sp. Ozone also significantly reduced the overall tumour promotional potency of the cyanobacterial extract, as indicated by a substantial reduction in the ability of the extract to inhibit GJIC and activate extracellular receptor kinase 1/2 (ERK1/2). Although comparable reduction of total organic carbon was achieved by ozone and chlorine treatment, chlorination was much less effective in removing MCs and reducing the effects on GJIC. Chlorination had a biphasic effect with an observed decrease of extract-induced activation of ERK1/2 at the lower chlorine doses; whereas at high doses of chlorine the by-products of chlorination actually induced the activation of ERK1/2. The extracts induced p38 activation, and chlorination was not effective in reversing this effect, while ozone did reverse this effect, albeit not as much as the activation of ERK1/2. Thus, ozone was effective in reducing the toxicity of cyanobacterial extracts while chlorination was not only lacking efficacy, but at high doses of chlorination further produced by-products that were equally toxic as the untreated samples. Our study indicates the value of using an effect-based approach to assess the efficacy of water treatment systems in removing toxins, and more specifically demonstrates that ozone was more effective at reducing the toxic potential of cyanobacterial-contaminated water.
ER  -

SOVADINOVÁ, Iva, Pavel BABICA, Ondřej ADAMOVSKÝ, Alla ALPATOVA, Volodymyr TARAMARA, Brad L. UPHAM a Luděk BLÁHA. Chlorination and ozonation differentially reduced the microcystin content and tumour promoting activity of a complex cyanobacterial extract. \textit{Advances in Oceanography and Limnology}. 2017, roč.~8, č.~1, s.~107-120. ISSN~1947-573X. Dostupné z: https://dx.doi.org/10.4081/aiol.2017.6342.

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