Subspecies-specific response to ACTH challenge test in the
house mouse (Mus musculus)

J 2017

Subspecies-specific response to ACTH challenge test in the house mouse (Mus musculus)

DANISZOVA, K.,, O. MIKULA, Miloš MACHOLÁN, I. POSPISILOVA, B. Voslajerova BIMOVA et. al.

Základní údaje

Originální název

Subspecies-specific response to ACTH challenge test in the house mouse (Mus musculus)

Autoři

DANISZOVA, K., (203 Česká republika, garant), O. MIKULA (203 Česká republika), Miloš MACHOLÁN (203 Česká republika, domácí), I. POSPISILOVA (203 Česká republika), B. Voslajerova BIMOVA (203 Česká republika) a Z. HIADLOVSKA (203 Česká republika)

Vydání

GENERAL AND COMPARATIVE ENDOCRINOLOGY, ACADEMIC PRESS INC ELSEVIER SCIENCE, 2017, 0016-6480

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30202 Endocrinology and metabolism

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.564

Kód RIV

RIV/00216224:14310/17:00100372

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.ygcen.2017.06.023

UT WoS

000409396400019

Klíčová slova anglicky

ACTH challenge; Endocrine activity; Corticosterone; Hormone metabolites; Mouse; Noninvasive monitoring

Štítky

NZ, rivok

Změněno: 12. 4. 2018 10:02, Ing. Nicole Zrilić

Anotace

V originále

Long term changes in hormonal levels of small and rare animal species can be effectively monitored by non-invasive methods such as immunoenzymatic analysis. These methods generally analyze metabolites instead of the hormone itself and thus do not invade the organism. However, they can be influenced by many factors, so before they are used, they need to be validated. For this purpose we used the "ACM challenge" test based on stimulating the animal's adrenocortical activity and measuring the consequent increase of the level of its glucocorticoid ("stress") hormones. Specifically, we measured concentrations of fecal glucocorticoid metabolites of two house mouse subspecies, Mus musculus musculus and M. m. domesticus. Using polyclonal antibody we investigated the mice's naturally occurring circadian fluctuation and their reaction to the adrenal stimulation. This study confirmed that the selected method is suitable for analysis of fecal corticosterone metabolites in the wild house mouse. More importantly, we revealed a subspecies-specific stress response at the level of corticosterone production: while the significant effect of ACTH was proved in both subspecies, a notable adrenocortical reaction was also elicited by injecting the saline solution in M. m. domesticus. Our results thus highlight the importance of considering potential cryptic variation within the species under study when designing projects on adrenal stress hormone assessments. (C) 2017 Elsevier Inc. All rights reserved.

Citovat

DANISZOVA, K.,, O. MIKULA, Miloš MACHOLÁN, I. POSPISILOVA, B. Voslajerova BIMOVA a Z. HIADLOVSKA. Subspecies-specific response to ACTH challenge test in the house mouse (Mus musculus). GENERAL AND COMPARATIVE ENDOCRINOLOGY. ACADEMIC PRESS INC ELSEVIER SCIENCE, 2017, roč. 252, OCT, s. 186-192. ISSN 0016-6480. Dostupné z: https://dx.doi.org/10.1016/j.ygcen.2017.06.023.

@article{1411206,
   author = {Daniszova, K., and Mikula, O. and Macholán, Miloš and Pospisilova, I. and Bimova, B. Voslajerova and Hiadlovska, Z.},
   article_number = {OCT},
   doi = {http://dx.doi.org/10.1016/j.ygcen.2017.06.023},
   keywords = {ACTH challenge; Endocrine activity; Corticosterone; Hormone metabolites; Mouse; Noninvasive monitoring},
   language = {eng},
   issn = {0016-6480},
   journal = {GENERAL AND COMPARATIVE ENDOCRINOLOGY},
   title = {Subspecies-specific response to ACTH challenge test in the house mouse (Mus musculus)},
   volume = {252},
   year = {2017}
}

TY  - JOUR
ID  - 1411206
AU  - Daniszova, K., - Mikula, O. - Macholán, Miloš - Pospisilova, I. - Bimova, B. Voslajerova - Hiadlovska, Z.
PY  - 2017
TI  - Subspecies-specific response to ACTH challenge test in the house mouse (Mus musculus)
JF  - GENERAL AND COMPARATIVE ENDOCRINOLOGY
VL  - 252
IS  - OCT
SP  - 186-192
EP  - 186-192
PB  - ACADEMIC PRESS INC ELSEVIER SCIENCE
SN  - 00166480
KW  - ACTH challenge
KW  - Endocrine activity
KW  - Corticosterone
KW  - Hormone metabolites
KW  - Mouse
KW  - Noninvasive monitoring
N2  - Long term changes in hormonal levels of small and rare animal species can be effectively monitored by non-invasive methods such as immunoenzymatic analysis. These methods generally analyze metabolites instead of the hormone itself and thus do not invade the organism. However, they can be influenced by many factors, so before they are used, they need to be validated. For this purpose we used the "ACM challenge" test based on stimulating the animal's adrenocortical activity and measuring the consequent increase of the level of its glucocorticoid ("stress") hormones. Specifically, we measured concentrations of fecal glucocorticoid metabolites of two house mouse subspecies, Mus musculus musculus and M. m. domesticus. Using polyclonal antibody we investigated the mice's naturally occurring circadian fluctuation and their reaction to the adrenal stimulation. This study confirmed that the selected method is suitable for analysis of fecal corticosterone metabolites in the wild house mouse. More importantly, we revealed a subspecies-specific stress response at the level of corticosterone production: while the significant effect of ACTH was proved in both subspecies, a notable adrenocortical reaction was also elicited by injecting the saline solution in M. m. domesticus. Our results thus highlight the importance of considering potential cryptic variation within the species under study when designing projects on adrenal stress hormone assessments. (C) 2017 Elsevier Inc. All rights reserved.
ER  -

DANISZOVA, K.,, O. MIKULA, Miloš MACHOLÁN, I. POSPISILOVA, B. Voslajerova BIMOVA a Z. HIADLOVSKA. Subspecies-specific response to ACTH challenge test in the house mouse (Mus musculus). \textit{GENERAL AND COMPARATIVE ENDOCRINOLOGY}. ACADEMIC PRESS INC ELSEVIER SCIENCE, 2017, roč.~252, OCT, s.~186-192. ISSN~0016-6480. Dostupné z: https://dx.doi.org/10.1016/j.ygcen.2017.06.023.

Podrobný výpis o publikaci