Staphylococcus edaphicus sp nov., isolated in Antarctica,
harbors the mecC gene and genomic islands with a suspected role
in adaptation to extreme environments

J 2018

Staphylococcus edaphicus sp nov., isolated in Antarctica, harbors the mecC gene and genomic islands with a suspected role in adaptation to extreme environments

PANTŮČEK, Roman, Ivo SEDLÁČEK, Adéla INDRÁKOVÁ, Veronika VRBOVSKÁ, Ivana MAŠLAŇOVÁ et. al.

Základní údaje

Originální název

Staphylococcus edaphicus sp nov., isolated in Antarctica, harbors the mecC gene and genomic islands with a suspected role in adaptation to extreme environments

Autoři

PANTŮČEK, Roman (203 Česká republika, garant, domácí), Ivo SEDLÁČEK (203 Česká republika, domácí), Adéla INDRÁKOVÁ (203 Česká republika, domácí), Veronika VRBOVSKÁ (703 Slovensko, domácí), Ivana MAŠLAŇOVÁ (203 Česká republika, domácí), Vojtěch KOVAŘOVIC (203 Česká republika, domácí), Pavel ŠVEC (203 Česká republika, domácí), Stanislava KRÁLOVÁ (703 Slovensko, domácí), Lucie KRIŠTOFOVÁ (203 Česká republika, domácí), Jana KEKLÁKOVÁ (203 Česká republika), Petr PETRÁŠ (203 Česká republika) a Jiří DOŠKAŘ (203 Česká republika, domácí)

Vydání

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Washington, D.C. American Society for Microbiology, 2018, 0099-2240

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.077

Kód RIV

RIV/00216224:14310/18:00102400

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1128/AEM.01746-17

UT WoS

000419048900009

Klíčová slova anglicky

coagulase-negative staphylococci; methicillin resistance; genomics; polyphasic taxonomy; pathogenicity islands; drug resistance evolution; beta-lactams; chromosomal islands; mobile genetic elements; penicillin-binding proteins; phylogenetic analysis

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 2. 5. 2019 14:10, Mgr. Tereza Miškechová

Anotace

V originále

Two Gram-stain-positive, coagulase-negative staphylococcal strains were isolated from abiotic sources comprising stone fragments and sandy soil in James Ross Island, Antarctica. Here, we describe properties of a novel species of the genus Staphylococcus that has a 16S rRNA gene sequence nearly identical to that of Staphylococcus saprophyticus. However, compared to S. saprophyticus and the next closest relatives, the new species demonstrates considerable phylogenetic distance at the whole-genome level, with an average nucleotide identity of <85% and inferred DNA-DNA hybridization of <30%. It forms a separate branch in the S. saprophyticus phylogenetic clade as confirmed by multilocus sequence analysis of six housekeeping genes, rpoB, hsp60, tuf, dnaJ, gap, and sod. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and key biochemical characteristics allowed these bacteria to be distinguished from their nearest phylogenetic neighbors. In contrast to S. saprophyticus subsp. saprophyticus, the novel strains are pyrrolidonyl arylamidase and beta-glucuronidase positive and beta-galactosidase negative, nitrate is reduced, and acid produced aerobically from D-mannose. Whole-genome sequencing of the 2.69-Mb large chromosome revealed the presence of a number of mobile genetic elements, including the 27-kb pseudo-staphylococcus cassette chromosome mec of strain P5085(T) (psi SCCmec(P5085)), harboring the mecC gene, two composite phage-inducible chromosomal islands probably essential to adaptation to extreme environments, and one complete and one defective prophage. Both strains are resistant to penicillin G, ampicillin, ceftazidime, methicillin, cefoxitin, and fosfomycin. We hypothesize that antibiotic resistance might represent an evolutionary advantage against beta-lactam producers, which are common in a polar environment. Based on these results, a novel species of the genus Staphylococcus is described and named Staphylococcus edaphicus sp. nov. The type strain is P5085(T) (= CCM 8730(T) = DSM 104441(T).

Návaznosti

LM2015078, projekt VaV

Název: Česká polární výzkumná infrastruktura (Akronym: CzechPolar2)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech Polar Research Infrastructure

NV16-29916A, projekt VaV

Název: Využití bakteriofágů v léčbě nozokomiálních infekcí spojených s multirezistencí či tvorbou biofilmu

Přiložené soubory

Appl._Environ._Microbiol.-2018-Pant__ek-.pdf

Požádat o autorskou verzi souboru

Citovat

PANTŮČEK, Roman, Ivo SEDLÁČEK, Adéla INDRÁKOVÁ, Veronika VRBOVSKÁ, Ivana MAŠLAŇOVÁ, Vojtěch KOVAŘOVIC, Pavel ŠVEC, Stanislava KRÁLOVÁ, Lucie KRIŠTOFOVÁ, Jana KEKLÁKOVÁ, Petr PETRÁŠ a Jiří DOŠKAŘ. Staphylococcus edaphicus sp nov., isolated in Antarctica, harbors the mecC gene and genomic islands with a suspected role in adaptation to extreme environments. APPLIED AND ENVIRONMENTAL MICROBIOLOGY. Washington, D.C.: American Society for Microbiology, 2018, roč. 84, č. 2, s. 1-13. ISSN 0099-2240. Dostupné z: https://dx.doi.org/10.1128/AEM.01746-17.

@article{1411217,
   author = {Pantůček, Roman and Sedláček, Ivo and Indráková, Adéla and Vrbovská, Veronika and Mašlaňová, Ivana and Kovařovic, Vojtěch and Švec, Pavel and Králová, Stanislava and Krištofová, Lucie and Kekláková, Jana and Petráš, Petr and Doškař, Jiří},
   article_location = {Washington, D.C.},
   article_number = {2},
   doi = {http://dx.doi.org/10.1128/AEM.01746-17},
   keywords = {coagulase-negative staphylococci; methicillin resistance; genomics; polyphasic taxonomy; pathogenicity islands; drug resistance evolution; beta-lactams; chromosomal islands; mobile genetic elements; penicillin-binding proteins; phylogenetic analysis},
   language = {eng},
   issn = {0099-2240},
   journal = {APPLIED AND ENVIRONMENTAL MICROBIOLOGY},
   title = {Staphylococcus edaphicus sp nov., isolated in Antarctica, harbors the mecC gene and genomic islands with a suspected role in adaptation to extreme environments},
   url = {http://aem.asm.org/content/84/2/e01746-17},
   volume = {84},
   year = {2018}
}

TY  - JOUR
ID  - 1411217
AU  - Pantůček, Roman - Sedláček, Ivo - Indráková, Adéla - Vrbovská, Veronika - Mašlaňová, Ivana - Kovařovic, Vojtěch - Švec, Pavel - Králová, Stanislava - Krištofová, Lucie - Kekláková, Jana - Petráš, Petr - Doškař, Jiří
PY  - 2018
TI  - Staphylococcus edaphicus sp nov., isolated in Antarctica, harbors the mecC gene and genomic islands with a suspected role in adaptation to extreme environments
JF  - APPLIED AND ENVIRONMENTAL MICROBIOLOGY
VL  - 84
IS  - 2
SP  - 1-13
EP  - 1-13
PB  - American Society for Microbiology
SN  - 00992240
KW  - coagulase-negative staphylococci
KW  - methicillin resistance
KW  - genomics
KW  - polyphasic taxonomy
KW  - pathogenicity islands
KW  - drug resistance evolution
KW  - beta-lactams
KW  - chromosomal islands
KW  - mobile genetic elements
KW  - penicillin-binding proteins
KW  - phylogenetic analysis
UR  - http://aem.asm.org/content/84/2/e01746-17
L2  - http://aem.asm.org/content/84/2/e01746-17
N2  - Two Gram-stain-positive, coagulase-negative staphylococcal strains were isolated from abiotic sources comprising stone fragments and sandy soil in James Ross Island, Antarctica. Here, we describe properties of a novel species of the genus Staphylococcus that has a 16S rRNA gene sequence nearly identical to that of Staphylococcus saprophyticus. However, compared to S. saprophyticus and the next closest relatives, the new species demonstrates considerable phylogenetic distance at the whole-genome level, with an average nucleotide identity of <85% and inferred DNA-DNA hybridization of <30%. It forms a separate branch in the S. saprophyticus phylogenetic clade as confirmed by multilocus sequence analysis of six housekeeping genes, rpoB, hsp60, tuf, dnaJ, gap, and sod. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and key biochemical characteristics allowed these bacteria to be distinguished from their nearest phylogenetic neighbors. In contrast to S. saprophyticus subsp. saprophyticus, the novel strains are pyrrolidonyl arylamidase and beta-glucuronidase positive and beta-galactosidase negative, nitrate is reduced, and acid produced aerobically from D-mannose. Whole-genome sequencing of the 2.69-Mb large chromosome revealed the presence of a number of mobile genetic elements, including the 27-kb pseudo-staphylococcus cassette chromosome mec of strain P5085(T) (psi SCCmec(P5085)), harboring the mecC gene, two composite phage-inducible chromosomal islands probably essential to adaptation to extreme environments, and one complete and one defective prophage. Both strains are resistant to penicillin G, ampicillin, ceftazidime, methicillin, cefoxitin, and fosfomycin. We hypothesize that antibiotic resistance might represent an evolutionary advantage against beta-lactam producers, which are common in a polar environment. Based on these results, a novel species of the genus Staphylococcus is described and named Staphylococcus edaphicus sp. nov. The type strain is P5085(T) (= CCM 8730(T) = DSM 104441(T).
ER  -

PANTŮČEK, Roman, Ivo SEDLÁČEK, Adéla INDRÁKOVÁ, Veronika VRBOVSKÁ, Ivana MAŠLAŇOVÁ, Vojtěch KOVAŘOVIC, Pavel ŠVEC, Stanislava KRÁLOVÁ, Lucie KRIŠTOFOVÁ, Jana KEKLÁKOVÁ, Petr PETRÁŠ a Jiří DOŠKAŘ. Staphylococcus edaphicus sp nov., isolated in Antarctica, harbors the mecC gene and genomic islands with a suspected role in adaptation to extreme environments. \textit{APPLIED AND ENVIRONMENTAL MICROBIOLOGY}. Washington, D.C.: American Society for Microbiology, 2018, roč.~84, č.~2, s.~1-13. ISSN~0099-2240. Dostupné z: https://dx.doi.org/10.1128/AEM.01746-17.

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