Organic Electrochemical Transistor Microplate for Real-Time
Cell Culture Monitoring

J 2017

Organic Electrochemical Transistor Microplate for Real-Time Cell Culture Monitoring

SALYK, Ota, Jan VÍTEČEK, Lukáš OMASTA, Eva ŠAFAŘÍKOVÁ, Stanislav STŘÍTESKÝ et. al.

Základní údaje

Originální název

Organic Electrochemical Transistor Microplate for Real-Time Cell Culture Monitoring

Autoři

SALYK, Ota (203 Česká republika, garant), Jan VÍTEČEK (203 Česká republika, domácí), Lukáš OMASTA (203 Česká republika), Eva ŠAFAŘÍKOVÁ (203 Česká republika, domácí), Stanislav STŘÍTESKÝ (203 Česká republika), Martin VALA (203 Česká republika) a Martin WEITER (203 Česká republika)

Vydání

Applied Sciences-Basel, BASEL, SWITZERLAND, MDPI AG, 2017, 2076-3417

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10400 1.4 Chemical sciences

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 1.689

Kód RIV

RIV/00216224:14310/17:00100375

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.3390/app7100998

UT WoS

000414457800036

Klíčová slova anglicky

OECT; screen printing; organic electrochemical transistor; PEDOT:PSS; microplate; multi-electrode array; cell culture

Štítky

NZ, rivok

Příznaky

Recenzováno

Změněno: 10. 4. 2018 11:52, Ing. Nicole Zrilić

Anotace

V originále

Human cell cultures provide a potentially powerful means for pharmacological and toxicological research. A microplate with a multielectrode array of 96 organic electrochemical transistors (OECTs) based on the semiconductive polymer poly(3,4-ethylenedioxythio-phene):poly(styrene sulfonic acid) PEDOT:PSS was developed and fabricated by the screen printing method. It consists of a microplate of a 12 × 8 chimney-well array with transistors on the bottom. The OECT is circular with a channel of 1.5 mm2 in the centre surrounded by the circular gate electrode. The device is designed for electrogenic cell monitoring. Simulations with the electrolyte revealed good electrical characteristics and indicated the setup information of the experimental conditions. A transconductance of g = 1.4 mS was achieved in the wide range of gate voltages Vgs = ±0.4 V when the drain potential Vds = -0.735 V was set and the long term relaxation was compensated for. The time constant 0.15 s limited by the channel-electrolyte charge electrical double layer (EDL) capacitance was measured. The device was tested on a 3T3 fibroblast cell culture and the sudden environmental changes were recorded. The living cells can be observed on the channel of the OECT and during electrical stimulation by gate voltage, as well as during the source current response.

Citovat

SALYK, Ota, Jan VÍTEČEK, Lukáš OMASTA, Eva ŠAFAŘÍKOVÁ, Stanislav STŘÍTESKÝ, Martin VALA a Martin WEITER. Organic Electrochemical Transistor Microplate for Real-Time Cell Culture Monitoring. Applied Sciences-Basel. BASEL, SWITZERLAND: MDPI AG, 2017, roč. 7, č. 10, s. nestránkováno, 10 s. ISSN 2076-3417. Dostupné z: https://dx.doi.org/10.3390/app7100998.

@article{1411252,
   author = {Salyk, Ota and Víteček, Jan and Omasta, Lukáš and Šafaříková, Eva and Stříteský, Stanislav and Vala, Martin and Weiter, Martin},
   article_location = {BASEL, SWITZERLAND},
   article_number = {10},
   doi = {http://dx.doi.org/10.3390/app7100998},
   keywords = {OECT; screen printing; organic electrochemical transistor; PEDOT:PSS; microplate; multi-electrode array; cell culture},
   language = {eng},
   issn = {2076-3417},
   journal = {Applied Sciences-Basel},
   title = {Organic Electrochemical Transistor Microplate for Real-Time Cell Culture Monitoring},
   url = {http://www.mdpi.com/2076-3417/7/10/998/htm},
   volume = {7},
   year = {2017}
}

TY  - JOUR
ID  - 1411252
AU  - Salyk, Ota - Víteček, Jan - Omasta, Lukáš - Šafaříková, Eva - Stříteský, Stanislav - Vala, Martin - Weiter, Martin
PY  - 2017
TI  - Organic Electrochemical Transistor Microplate for Real-Time Cell Culture Monitoring
JF  - Applied Sciences-Basel
VL  - 7
IS  - 10
SP  - nestránkováno
EP  - nestránkováno
PB  - MDPI AG
SN  - 20763417
KW  - OECT
KW  - screen printing
KW  - organic electrochemical transistor
KW  - PEDOT:PSS
KW  - microplate
KW  - multi-electrode array
KW  - cell culture
UR  - http://www.mdpi.com/2076-3417/7/10/998/htm
L2  - http://www.mdpi.com/2076-3417/7/10/998/htm
N2  - Human cell cultures provide a potentially powerful means for pharmacological and toxicological research. A microplate with a multielectrode array of 96 organic electrochemical transistors (OECTs) based on the semiconductive polymer poly(3,4-ethylenedioxythio-phene):poly(styrene sulfonic acid) PEDOT:PSS was developed and fabricated by the screen printing method. It consists of a microplate of a 12 × 8 chimney-well array with transistors on the bottom. The OECT is circular with a channel of 1.5 mm2 in the centre surrounded by the circular gate electrode. The device is designed for electrogenic cell monitoring. Simulations with the electrolyte revealed good electrical characteristics and indicated the setup information of the experimental conditions. A transconductance of g = 1.4 mS was achieved in the wide range of gate voltages Vgs = ±0.4 V when the drain potential Vds = -0.735 V was set and the long term relaxation was compensated for. The time constant 0.15 s limited by the channel-electrolyte charge electrical double layer (EDL) capacitance was measured. The device was tested on a 3T3 fibroblast cell culture and the sudden environmental changes were recorded. The living cells can be observed on the channel of the OECT and during electrical stimulation by gate voltage, as well as during the source current response.
ER  -

SALYK, Ota, Jan VÍTEČEK, Lukáš OMASTA, Eva ŠAFAŘÍKOVÁ, Stanislav STŘÍTESKÝ, Martin VALA a Martin WEITER. Organic Electrochemical Transistor Microplate for Real-Time Cell Culture Monitoring. \textit{Applied Sciences-Basel}. BASEL, SWITZERLAND: MDPI AG, 2017, roč.~7, č.~10, s.~nestránkováno, 10 s. ISSN~2076-3417. Dostupné z: https://dx.doi.org/10.3390/app7100998.

Podrobný výpis o publikaci