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@article{1411854,
author = {Šimečková, Šárka and Fedr, Radek and Remšík, Ján and Kahounová, Z. and Slabáková, Eva and Souček, Karel},
article_location = {HOBOKEN},
article_number = {2},
doi = {http://dx.doi.org/10.1002/cyto.a.23295},
keywords = {flow cytometry; multiparametric analysis; DNA damage; apoptosis; proliferation; immunophenotyping},
language = {eng},
issn = {1552-4922},
journal = {Cytometry Part A},
title = {Multiparameter cytometric analysis of complex cellular response},
url = {http://dx.doi.org/10.1002/cyto.a.23295},
volume = {93A},
year = {2018}
}
TY - JOUR
ID - 1411854
AU - Šimečková, Šárka - Fedr, Radek - Remšík, Ján - Kahounová, Z. - Slabáková, Eva - Souček, Karel
PY - 2018
TI - Multiparameter cytometric analysis of complex cellular response
JF - Cytometry Part A
VL - 93A
IS - 2
SP - 239-248
EP - 239-248
PB - John Wiley & Sons
SN - 15524922
KW - flow cytometry
KW - multiparametric analysis
KW - DNA damage
KW - apoptosis
KW - proliferation
KW - immunophenotyping
UR - http://dx.doi.org/10.1002/cyto.a.23295
N2 - Complex analysis of cellular responses after experimental treatment is important for screening, mechanistic understanding of treatment effects, and the identification of sensitive and resistant cell phenotypes. Modern multicolor flow cytometry has demonstrated its power for such analyses. Here, we introduce a multiparametric protocol for complex analysis of cytokinetics by the simultaneous detection of seven fluorescence parameters. This analysis includes the detection of two surface markers for immunophenotyping, analysis of proliferation based on the cell cycle and the measurement of incorporated nucleoside analogue 5-ethynyl-2-deoxyuridine (EdU) in newly synthesized DNA, analysis of DNA damage using an anti-phospho-histone H2A.X (Ser139) antibody, and determination of cell death using a fixable viability probe and intracellular detection of caspase-3 activation. To demonstrate the applicability of this protocol for the analysis of heterogeneous and complex cell responses, we used different treatments and model cell lines. We demonstrated that this protocol has the potential to provide complex and simultaneous analysis of cytokinetics and analyze the heterogeneity of the response at the single-cell level. (c) 2017 International Society for Advancement of Cytometry
ER -
ŠIMEČKOVÁ, Šárka, Radek FEDR, Ján REMŠÍK, Z. KAHOUNOVÁ, Eva SLABÁKOVÁ a Karel SOUČEK. Multiparameter cytometric analysis of complex cellular response. \textit{Cytometry Part A}. HOBOKEN: John Wiley \&{} Sons, 2018, roč.~93A, č.~2, s.~239-248. ISSN~1552-4922. Dostupné z: https://dx.doi.org/10.1002/cyto.a.23295.
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