Structural and Surface Compatibility Study of Modified
Electrospun Poly(epsilon-caprolactone) (PCL) Composites for
Skin Tissue Engineering

J 2017

Structural and Surface Compatibility Study of Modified Electrospun Poly(epsilon-caprolactone) (PCL) Composites for Skin Tissue Engineering

GHOSAL, Kajal, Anton MANAKHOV, Lenka ZAJÍČKOVÁ a Sabu THOMAS

Základní údaje

Originální název

Structural and Surface Compatibility Study of Modified Electrospun Poly(epsilon-caprolactone) (PCL) Composites for Skin Tissue Engineering

Autoři

GHOSAL, Kajal (356 Indie), Anton MANAKHOV (643 Rusko, domácí), Lenka ZAJÍČKOVÁ (203 Česká republika, garant, domácí) a Sabu THOMAS (356 Indie)

Vydání

AAPS PHARMSCITECH, NEW YORK, SPRINGER, 2017, 1530-9932

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10305 Fluids and plasma physics

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.666

Kód RIV

RIV/00216224:14740/17:00100438

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1208/s12249-016-0500-8

UT WoS

000392104200009

Klíčová slova anglicky

compatibility study; composites; electrospinning; PCL; skin tissue engineering

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 21. 3. 2018 09:49, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

In this study, biodegradable poly(epsilon-caprolactone) (PCL) nanofibers (PCL-NF), collagen-coated PCL nanofibers (Col-c-PCL), and titanium dioxide-incorporated PCL (TiO2-i-PCL) nanofibers were prepared by electrospinning technique to study the surface and structural compatibility of these scaffolds for skin tisuue engineering. Collagen coating over the PCL nanofibers was done by electrospinning process. Morphology of PCL nanofibers in electrospinning was investigated at different voltages and at different concentrations of PCL. The morphology, interaction between different materials, surface property, and presence of TiO2 were studied by scanning electron microscopy (SEM), Fourier transform IR spectroscopy (FTIR), contact angle measurement, energy dispersion X-ray spectroscopy (EDX), and X-ray photoelectron spectroscopy (XPS). MTT assay and cell adhesion study were done to check biocompatibilty of these scaffolds. SEM study confirmed the formation of nanofibers without beads. FTIR proved presence of collagen on PCL scaffold, and contact angle study showed increment of hydrophilicity of Col-c-PCL and TiO2-i-PCL due to collagen coating and incorporation of TiO2, respectively. EDX and XPS studies revealed distribution of entrapped TiO2 at molecular level. MTT assay and cell adhesion study using L929 fibroblast cell line proved viability of cells with attachment of fibroblasts over the scaffold. Thus, in a nutshell, we can conclude from the outcomes of our investigational works that such composite can be considered as a tissue engineered construct for skin wound healing.

Návaznosti

LD15150, projekt VaV

Název: Elektrostaticky zvlákněná biodegradabilní nanovlákna pokrytá plazmově připravenými aminovými vrstvami pro využití v biomedicíně

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Elektrostaticky zvlákněná biodegradabilní nanovlákna pokrytá plazmově připravenými aminovými vrstvami pro využití v biomedicíně

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

3SGA5652, interní kód MU

Název: Bioactive Nanofibers Prepared by Electrospinning and Plasma Technologies (Akronym: BioFibPlas)

Investor: Jihomoravský kraj, Bioactive Nanofibers Prepared by Electrospinning and Plasma Technologies, Granty pro zahraniční vědce

Citovat

GHOSAL, Kajal, Anton MANAKHOV, Lenka ZAJÍČKOVÁ a Sabu THOMAS. Structural and Surface Compatibility Study of Modified Electrospun Poly(epsilon-caprolactone) (PCL) Composites for Skin Tissue Engineering. AAPS PHARMSCITECH. NEW YORK: SPRINGER, 2017, roč. 18, č. 1, s. 72-81. ISSN 1530-9932. Dostupné z: https://dx.doi.org/10.1208/s12249-016-0500-8.

@article{1411915,
   author = {Ghosal, Kajal and Manakhov, Anton and Zajíčková, Lenka and Thomas, Sabu},
   article_location = {NEW YORK},
   article_number = {1},
   doi = {http://dx.doi.org/10.1208/s12249-016-0500-8},
   keywords = {compatibility study; composites; electrospinning; PCL; skin tissue engineering},
   language = {eng},
   issn = {1530-9932},
   journal = {AAPS PHARMSCITECH},
   title = {Structural and Surface Compatibility Study of Modified Electrospun Poly(epsilon-caprolactone) (PCL) Composites for Skin Tissue Engineering},
   url = {https://link.springer.com/content/pdf/10.1208%2Fs12249-016-0500-8.pdf},
   volume = {18},
   year = {2017}
}

TY  - JOUR
ID  - 1411915
AU  - Ghosal, Kajal - Manakhov, Anton - Zajíčková, Lenka - Thomas, Sabu
PY  - 2017
TI  - Structural and Surface Compatibility Study of Modified Electrospun Poly(epsilon-caprolactone) (PCL) Composites for Skin Tissue Engineering
JF  - AAPS PHARMSCITECH
VL  - 18
IS  - 1
SP  - 72-81
EP  - 72-81
PB  - SPRINGER
SN  - 15309932
KW  - compatibility study
KW  - composites
KW  - electrospinning
KW  - PCL
KW  - skin tissue engineering
UR  - https://link.springer.com/content/pdf/10.1208%2Fs12249-016-0500-8.pdf
L2  - https://link.springer.com/content/pdf/10.1208%2Fs12249-016-0500-8.pdf
N2  - In this study, biodegradable poly(epsilon-caprolactone) (PCL) nanofibers (PCL-NF), collagen-coated PCL nanofibers (Col-c-PCL), and titanium dioxide-incorporated PCL (TiO2-i-PCL) nanofibers were prepared by electrospinning technique to study the surface and structural compatibility of these scaffolds for skin tisuue engineering. Collagen coating over the PCL nanofibers was done by electrospinning process. Morphology of PCL nanofibers in electrospinning was investigated at different voltages and at different concentrations of PCL. The morphology, interaction between different materials, surface property, and presence of TiO2 were studied by scanning electron microscopy (SEM), Fourier transform IR spectroscopy (FTIR), contact angle measurement, energy dispersion X-ray spectroscopy (EDX), and X-ray photoelectron spectroscopy (XPS). MTT assay and cell adhesion study were done to check biocompatibilty of these scaffolds. SEM study confirmed the formation of nanofibers without beads. FTIR proved presence of collagen on PCL scaffold, and contact angle study showed increment of hydrophilicity of Col-c-PCL and TiO2-i-PCL due to collagen coating and incorporation of TiO2, respectively. EDX and XPS studies revealed distribution of entrapped TiO2 at molecular level. MTT assay and cell adhesion study using L929 fibroblast cell line proved viability of cells with attachment of fibroblasts over the scaffold. Thus, in a nutshell, we can conclude from the outcomes of our investigational works that such composite can be considered as a tissue engineered construct for skin wound healing.
ER  -

GHOSAL, Kajal, Anton MANAKHOV, Lenka ZAJÍČKOVÁ a Sabu THOMAS. Structural and Surface Compatibility Study of Modified Electrospun Poly(epsilon-caprolactone) (PCL) Composites for Skin Tissue Engineering. \textit{AAPS PHARMSCITECH}. NEW YORK: SPRINGER, 2017, roč.~18, č.~1, s.~72-81. ISSN~1530-9932. Dostupné z: https://dx.doi.org/10.1208/s12249-016-0500-8.

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