| SIMKOVA, D., G. KHARAISHVILI, G. KORINKOVA, T. OZDIAN, T. SUCHANKOVA-KLEPLOVA, T. SOUKUP, M. KRUPKA, A. GALANDAKOVA, P. DZUBAK, M. JANIKOVA, J. NAVRATIL, Z. KAHOUNOVA, Karel SOUČEK and J. BOUCHAL. The dual role of asporin in breast cancer progression. Oncotarget. Albany: Impact Journals, 2016, vol. 7, No 32, p. 52045-52060. ISSN 1949-2553. Available from: https://dx.doi.org/10.18632/oncotarget.10471. |
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@article{1412022,
author = {Simkova, D. and Kharaishvili, G. and Korinkova, G. and Ozdian, T. and SuchankovaandKleplova, T. and Soukup, T. and Krupka, M. and Galandakova, A. and Dzubak, P. and Janikova, M. and Navratil, J. and Kahounova, Z. and Souček, Karel and Bouchal, J.},
article_location = {Albany},
article_number = {32},
doi = {http://dx.doi.org/10.18632/oncotarget.10471},
keywords = {asporin; 3D cultivation; stiffness; grade; breast cancer},
language = {eng},
issn = {1949-2553},
journal = {Oncotarget},
title = {The dual role of asporin in breast cancer progression},
volume = {7},
year = {2016}
}
TY - JOUR
ID - 1412022
AU - Simkova, D. - Kharaishvili, G. - Korinkova, G. - Ozdian, T. - Suchankova-Kleplova, T. - Soukup, T. - Krupka, M. - Galandakova, A. - Dzubak, P. - Janikova, M. - Navratil, J. - Kahounova, Z. - Souček, Karel - Bouchal, J.
PY - 2016
TI - The dual role of asporin in breast cancer progression
JF - Oncotarget
VL - 7
IS - 32
SP - 52045-52060
EP - 52045-52060
PB - Impact Journals
SN - 19492553
KW - asporin
KW - 3D cultivation
KW - stiffness
KW - grade
KW - breast cancer
N2 - Asporin has been reported as a tumor suppressor in breast cancer, while asporin-activated invasion has been described in gastric cancer. According to our in silico search, high asporin expresion associates with significantly better relapse free survival (RFS) in patients with low-grade tumors but RFS is significantly worse in patients with grade 3 tumors. In line with other studies, we have confirmed asporin expression by RNA scope in situ hybridization in cancer associated fibroblasts. We have also found asporin expression in the Hs578T breast cancer cell line which we confirmed by quantitative RT-PCR and western blotting. From multiple testing, we found that asporin can be downregulated by bone morphogenetic protein 4 while upregulation may be facilited by serum-free cultivation or by three dimensional growth in stiff Alvetex scaffold. Downregulation by shRNA inhibited invasion of Hs578T as well as of CAFs and T47D cells. Invasion of asporin-negative MDA-MB-231 and BT549 breast cancer cells through collagen type I was enhanced by recombinant asporin. Besides other investigations, large scale analysis of aspartic acid repeat polymorphism will be needed for clarification of the asporin dual role in progression of breast cancer.
ER -
SIMKOVA, D., G. KHARAISHVILI, G. KORINKOVA, T. OZDIAN, T. SUCHANKOVA-KLEPLOVA, T. SOUKUP, M. KRUPKA, A. GALANDAKOVA, P. DZUBAK, M. JANIKOVA, J. NAVRATIL, Z. KAHOUNOVA, Karel SOUČEK and J. BOUCHAL. The dual role of asporin in breast cancer progression. \textit{Oncotarget}. Albany: Impact Journals, 2016, vol.~7, No~32, p.~52045-52060. ISSN~1949-2553. Available from: https://dx.doi.org/10.18632/oncotarget.10471.
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