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@article{1412045, author = {Sampels, S. and Kroupova, H.K. and Linhartová, Pavla}, article_location = {OXFORD}, article_number = {OCT}, doi = {http://dx.doi.org/10.1016/j.tiv.2017.08.003}, keywords = {EPA; DHA; Hepatoblastoma cells; mt1 g; mt1 m; Cadmium}, language = {eng}, issn = {0887-2333}, journal = {Toxicology in vitro}, title = {Effect of cadmium on uptake of iron, zinc and copper and mRNA expression of metallothioneins in HepG2 cells in vitro}, url = {https://www.sciencedirect.com/science/article/pii/S0887233317302199?via%3Dihub}, volume = {44}, year = {2017} }
TY - JOUR ID - 1412045 AU - Sampels, S. - Kroupova, H.K. - Linhartová, Pavla PY - 2017 TI - Effect of cadmium on uptake of iron, zinc and copper and mRNA expression of metallothioneins in HepG2 cells in vitro JF - Toxicology in vitro VL - 44 IS - OCT SP - 372-376 EP - 372-376 PB - PERGAMON-ELSEVIER SCIENCE LTD SN - 08872333 KW - EPA KW - DHA KW - Hepatoblastoma cells KW - mt1 g KW - mt1 m KW - Cadmium UR - https://www.sciencedirect.com/science/article/pii/S0887233317302199?via%3Dihub L2 - https://www.sciencedirect.com/science/article/pii/S0887233317302199?via%3Dihub N2 - The intake of cadmium contaminated fish was mimicked by incubating human hepatoblastoma cells (Cell line HepG2) with a combination of different levels of cadmium (0-5 mu M) plus the n-3 fatty acids docosahexaenoic acid and eicosapentaenoic acid, which are typical for fish. Uptake of cadmium, iron, copper and zinc was measured by ICP-MS. In addition mRNA expression of two metallothioneins (mt1 g and mt1 m) was evaluated by real-time PCR. The obtained data shows that the presence of cadmium increases the uptake of iron and zinc into the HepG2 cells while the uptake of copper remains unaffected. The presence of the chosen fatty acids did not affect the uptake of either cadmium or iron, zinc and copper. The presence of already 1 mu M cadmium increased the mRNA expression of mt1 g and mt1 m significantly, while the fatty acids did not interfere with the effect of cadmium. ER -
SAMPELS, S., H.K. KROUPOVA a Pavla LINHARTOVÁ. Effect of cadmium on uptake of iron, zinc and copper and mRNA expression of metallothioneins in HepG2 cells in vitro. \textit{Toxicology in vitro}. OXFORD: PERGAMON-ELSEVIER SCIENCE LTD, 2017, roč.~44, OCT, s.~372-376. ISSN~0887-2333. Dostupné z: https://dx.doi.org/10.1016/j.tiv.2017.08.003.
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