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@article{1412045,
author = {Sampels, S. and Kroupova, H.K. and Linhartová, Pavla},
article_location = {OXFORD},
article_number = {OCT},
doi = {http://dx.doi.org/10.1016/j.tiv.2017.08.003},
keywords = {EPA; DHA; Hepatoblastoma cells; mt1 g; mt1 m; Cadmium},
language = {eng},
issn = {0887-2333},
journal = {Toxicology in vitro},
title = {Effect of cadmium on uptake of iron, zinc and copper and mRNA expression of metallothioneins in HepG2 cells in vitro},
url = {https://www.sciencedirect.com/science/article/pii/S0887233317302199?via%3Dihub},
volume = {44},
year = {2017}
}
TY - JOUR
ID - 1412045
AU - Sampels, S. - Kroupova, H.K. - Linhartová, Pavla
PY - 2017
TI - Effect of cadmium on uptake of iron, zinc and copper and mRNA expression of metallothioneins in HepG2 cells in vitro
JF - Toxicology in vitro
VL - 44
IS - OCT
SP - 372-376
EP - 372-376
PB - PERGAMON-ELSEVIER SCIENCE LTD
SN - 08872333
KW - EPA
KW - DHA
KW - Hepatoblastoma cells
KW - mt1 g
KW - mt1 m
KW - Cadmium
UR - https://www.sciencedirect.com/science/article/pii/S0887233317302199?via%3Dihub
L2 - https://www.sciencedirect.com/science/article/pii/S0887233317302199?via%3Dihub
N2 - The intake of cadmium contaminated fish was mimicked by incubating human hepatoblastoma cells (Cell line HepG2) with a combination of different levels of cadmium (0-5 mu M) plus the n-3 fatty acids docosahexaenoic acid and eicosapentaenoic acid, which are typical for fish. Uptake of cadmium, iron, copper and zinc was measured by ICP-MS. In addition mRNA expression of two metallothioneins (mt1 g and mt1 m) was evaluated by real-time PCR. The obtained data shows that the presence of cadmium increases the uptake of iron and zinc into the HepG2 cells while the uptake of copper remains unaffected. The presence of the chosen fatty acids did not affect the uptake of either cadmium or iron, zinc and copper. The presence of already 1 mu M cadmium increased the mRNA expression of mt1 g and mt1 m significantly, while the fatty acids did not interfere with the effect of cadmium.
ER -
SAMPELS, S., H.K. KROUPOVA a Pavla LINHARTOVÁ. Effect of cadmium on uptake of iron, zinc and copper and mRNA expression of metallothioneins in HepG2 cells in vitro. \textit{Toxicology in vitro}. OXFORD: PERGAMON-ELSEVIER SCIENCE LTD, 2017, roč.~44, OCT, s.~372-376. ISSN~0887-2333. Dostupné z: https://dx.doi.org/10.1016/j.tiv.2017.08.003.
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