Polymorphisms in genes coding purinoreceptor and
osteoprotegerin in relation to external apical root resorption
in patients after orthodontic treatment

a 2018

Polymorphisms in genes coding purinoreceptor and osteoprotegerin in relation to external apical root resorption in patients after orthodontic treatment

VRANKOVÁ, Zuzana, Martina SIROTKOVÁ, Petra BOŘILOVÁ LINHARTOVÁ, Pavlína ČERNOCHOVÁ, Jakub KAŠTOVSKÝ et. al.

Základní údaje

Originální název

Polymorphisms in genes coding purinoreceptor and osteoprotegerin in relation to external apical root resorption in patients after orthodontic treatment

Autoři

VRANKOVÁ, Zuzana (703 Slovensko), Martina SIROTKOVÁ (703 Slovensko), Petra BOŘILOVÁ LINHARTOVÁ (203 Česká republika, domácí), Pavlína ČERNOCHOVÁ (203 Česká republika), Jakub KAŠTOVSKÝ (203 Česká republika) a Lydie IZAKOVIČOVÁ HOLLÁ (203 Česká republika)

Vydání

International spring meeting 2018, Societa Italiana di Ortodonzia, SIDO, Naples, Italy. Best Poster Award (Free Topic). 2018

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

30101 Human genetics

Stát vydavatele

Itálie

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14110/18:00100870

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

purinergic receptor; diabetes mellitus; periodontitis; chronic inflammation; gene variability; polymorphism

Změněno: 15. 1. 2019 12:22, doc. RNDr. Petra Bořilová Linhartová, Ph.D., MBA

Anotace

V originále

Background: A bidirectional relationship between chronic periodontitis (CP) and diabetes mellitus type 2 (T2DM), characterized by low-grade inflammation, was previously described. Purinergic signaling plays a role in the activation of multiprotein intracellular complexes called inflammasomes, which control release of potent proinflammatory cytokines. The aim of the present study was to analyze two purinergic receptor (P2RX7) gene variants with gain-of-function effect in patients T2DM and/or CP in Czech population. Subjects and methods: Totally, 473 unrelated subjects were included in this case-control study. Genomic DNA of 208 patients with CP, 83 patients with T2DM+CP and 182 systemically healthy non-periodontitis controls were genotyped using the qPCR TaqMan method for His155Tyr (rs208294, C/T) and Ala348Thr (rs1718119, A/G) polymorphisms in the P2RX7 gene. Results: No significant differences in allele and/or genotype frequencies of P2RX7 His155Tyr between cases and controls were found. However, the G allele and GG genotype of P2RX7 Ala348Thr variant were marginally associated with CP (P=0.065 and P=0.079, respectively). In addition, the GG genotype, encoding Ala/Ala in amino acid sequence, was negatively correlated with levels of glycated hemoglobin (HbA1c) in T2DM patients (P<0.01); patients with Thr/Thr genotype that is associated with gain-of-function had the highest levels of HbA1c. Conclusions: Although only marginal association of polymorphism P2RX7 Ala348Thr with susceptibility to CP in the Czech population was found, purinergic signaling via P2RX7 gene variability might influence glucose regulation.

Návaznosti

GB14-37368G, projekt VaV

Název: Centrum orofaciálního vývoje a regenerace

Investor: Grantová agentura ČR, Centrum orofaciálního vývoje a regenerace

MUNI/A/1258/2015, interní kód MU

Název: Analýza klinických, mikrobiálních a genetických faktorů v etiopatogenezi vybraných patologických stavů v dutině ústní

Investor: Masarykova univerzita, Analýza klinických, mikrobiálních a genetických faktorů v etiopatogenezi vybraných patologických stavů v dutině ústní, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

ROZV/24/LF/2016, interní kód MU

Název: LF - Příspěvek IP 2016

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, LF - Příspěvek IP 2016

Citovat

VRANKOVÁ, Zuzana, Martina SIROTKOVÁ, Petra BOŘILOVÁ LINHARTOVÁ, Pavlína ČERNOCHOVÁ, Jakub KAŠTOVSKÝ a Lydie IZAKOVIČOVÁ HOLLÁ. Polymorphisms in genes coding purinoreceptor and osteoprotegerin in relation to external apical root resorption in patients after orthodontic treatment. In International spring meeting 2018, Societa Italiana di Ortodonzia, SIDO, Naples, Italy. Best Poster Award (Free Topic). 2018.

@proceedings{1412115,
   author = {Vranková, Zuzana and Sirotková, Martina and Bořilová Linhartová, Petra and Černochová, Pavlína and Kaštovský, Jakub and Izakovičová Hollá, Lydie},
   booktitle = {International spring meeting 2018, Societa Italiana di Ortodonzia, SIDO, Naples, Italy. Best Poster Award (Free Topic).},
   keywords = {purinergic receptor; diabetes mellitus; periodontitis; chronic inflammation; gene variability; polymorphism},
   language = {eng},
   title = {Polymorphisms in genes coding purinoreceptor and osteoprotegerin in relation to external apical root resorption in patients after orthodontic treatment},
   year = {2018}
}

TY  - CONF
ID  - 1412115
AU  - Vranková, Zuzana - Sirotková, Martina - Bořilová Linhartová, Petra - Černochová, Pavlína - Kaštovský, Jakub - Izakovičová Hollá, Lydie
PY  - 2018
TI  - Polymorphisms in genes coding purinoreceptor and osteoprotegerin in relation to external apical root resorption in patients after orthodontic treatment
KW  - purinergic receptor
KW  - diabetes mellitus
KW  - periodontitis
KW  - chronic inflammation
KW  - gene variability
KW  - polymorphism
N2  - Background: A bidirectional relationship between chronic periodontitis (CP) and diabetes mellitus type 2 (T2DM), characterized by low-grade inflammation, was previously described. Purinergic signaling plays a role in the activation of multiprotein intracellular complexes called inflammasomes, which control release of potent proinflammatory cytokines. The aim of the present study was to analyze two purinergic receptor (P2RX7) gene variants with gain-of-function effect in patients T2DM and/or CP in Czech population. Subjects and methods: Totally, 473 unrelated subjects were included in this case-control study. Genomic DNA of 208 patients with CP, 83 patients with T2DM+CP and 182 systemically healthy non-periodontitis controls were genotyped using the qPCR TaqMan method for His155Tyr (rs208294, C/T) and Ala348Thr (rs1718119, A/G) polymorphisms in the P2RX7 gene. Results: No significant differences in allele and/or genotype frequencies of P2RX7 His155Tyr between cases and controls were found. However, the G allele and GG genotype of P2RX7 Ala348Thr variant were marginally associated with CP (P=0.065 and P=0.079, respectively). In addition, the GG genotype, encoding Ala/Ala in amino acid sequence, was negatively correlated with levels of glycated hemoglobin (HbA1c) in T2DM patients (P<0.01); patients with Thr/Thr genotype that is associated with gain-of-function had the highest levels of HbA1c. Conclusions: Although only marginal association of polymorphism P2RX7 Ala348Thr with susceptibility to CP in the Czech population was found, purinergic signaling via P2RX7 gene variability might influence glucose regulation.
ER  -

VRANKOVÁ, Zuzana, Martina SIROTKOVÁ, Petra BOŘILOVÁ LINHARTOVÁ, Pavlína ČERNOCHOVÁ, Jakub KAŠTOVSKÝ a Lydie IZAKOVIČOVÁ HOLLÁ. Polymorphisms in genes coding purinoreceptor and osteoprotegerin in relation to external apical root resorption in patients after orthodontic treatment. In \textit{International spring meeting 2018, Societa Italiana di Ortodonzia, SIDO, Naples, Italy. Best Poster Award (Free Topic).}. 2018.

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