i-Motif of cytosine-rich human telomere DNA fragments
containing natural base lesions

J 2018

i-Motif of cytosine-rich human telomere DNA fragments containing natural base lesions

DVOŘÁKOVÁ, Zuzana, Daniel RENČIUK, Iva KEJNOVSKÁ, Petra ŠKOLÁKOVÁ, Klára BEDNÁŘOVÁ et. al.

Základní údaje

Originální název

i-Motif of cytosine-rich human telomere DNA fragments containing natural base lesions

Autoři

DVOŘÁKOVÁ, Zuzana, Daniel RENČIUK, Iva KEJNOVSKÁ, Petra ŠKOLÁKOVÁ, Klára BEDNÁŘOVÁ, J SAGI a Michaela VORLÍČKOVÁ

Vydání

Nucleic Acids Research, Oxford, Oxford University Press, 2018, 0305-1048

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 11.147

DOI

http://dx.doi.org/10.1093/nar/gky035

UT WoS

000426293300012

Změněno: 9. 4. 2018 15:13, Mgr. Zuzana Dvořáková, Ph.D.

Anotace

V originále

i-Motif (iM) is a four stranded DNA structure formed by cytosine-rich sequences, which are often present in functionally important parts of the genome such as promoters of genes and telomeres. Using electronic circular dichroism and UV absorption spectroscopies and electrophoretic methods, we examined the effect of four naturally occurring DNA base lesions on the folding and stability of the iM formed by the human telomere DNA sequence (C3TAA) 3C3T. The results demonstrate that the TAA loop lesions, the apurinic site and 8-oxoadenine substituting for adenine, and the 5-hydroxymethyluracil substituting for thymine only marginally disturb the formation of iM. The presence of uracil, which is formed by enzymatic or spontaneous deamination of cytosine, shifts iM formation towards substantially more acidic pH values and simultaneously distinctly reduces iM stability. This effect depends on the position of the damage sites in the sequence. The results have enabled us to formulate additional rules for iM formation.

Citovat

DVOŘÁKOVÁ, Zuzana, Daniel RENČIUK, Iva KEJNOVSKÁ, Petra ŠKOLÁKOVÁ, Klára BEDNÁŘOVÁ, J SAGI a Michaela VORLÍČKOVÁ. i-Motif of cytosine-rich human telomere DNA fragments containing natural base lesions. Nucleic Acids Research. Oxford: Oxford University Press, 2018, roč. 46, č. 4, s. 1624-1634. ISSN 0305-1048. Dostupné z: https://dx.doi.org/10.1093/nar/gky035.

@article{1413677,
   author = {Dvořáková, Zuzana and Renčiuk, Daniel and Kejnovská, Iva and Školáková, Petra and Bednářová, Klára and Sagi, J and Vorlíčková, Michaela},
   article_location = {Oxford},
   article_number = {4},
   doi = {http://dx.doi.org/10.1093/nar/gky035},
   language = {eng},
   issn = {0305-1048},
   journal = {Nucleic Acids Research},
   title = {i-Motif of cytosine-rich human telomere DNA fragments containing natural base lesions},
   url = {http://dx.doi.org/10.1093/nar/gky035},
   volume = {46},
   year = {2018}
}

TY  - JOUR
ID  - 1413677
AU  - Dvořáková, Zuzana - Renčiuk, Daniel - Kejnovská, Iva - Školáková, Petra - Bednářová, Klára - Sagi, J - Vorlíčková, Michaela
PY  - 2018
TI  - i-Motif of cytosine-rich human telomere DNA fragments containing natural base lesions
JF  - Nucleic Acids Research
VL  - 46
IS  - 4
SP  - 1624-1634
EP  - 1624-1634
PB  - Oxford University Press
SN  - 03051048
UR  - http://dx.doi.org/10.1093/nar/gky035
L2  - http://dx.doi.org/10.1093/nar/gky035
N2  - i-Motif (iM) is a four stranded DNA structure formed by cytosine-rich sequences, which are often present in functionally important parts of the genome such as promoters of genes and telomeres. Using electronic circular dichroism and UV absorption spectroscopies and electrophoretic methods, we examined the effect of four naturally occurring DNA base lesions on the folding and stability of the iM formed by the human telomere DNA sequence (C3TAA) 3C3T. The results demonstrate that the TAA loop lesions, the apurinic site and 8-oxoadenine substituting for adenine, and the 5-hydroxymethyluracil substituting for thymine only marginally disturb the formation of iM. The presence of uracil, which is formed by enzymatic or spontaneous deamination of cytosine, shifts iM formation towards substantially more acidic pH values and simultaneously distinctly reduces iM stability. This effect depends on the position of the damage sites in the sequence. The results have enabled us to formulate additional rules for iM formation.
ER  -

DVOŘÁKOVÁ, Zuzana, Daniel RENČIUK, Iva KEJNOVSKÁ, Petra ŠKOLÁKOVÁ, Klára BEDNÁŘOVÁ, J SAGI a Michaela VORLÍČKOVÁ. i-Motif of cytosine-rich human telomere DNA fragments containing natural base lesions. \textit{Nucleic Acids Research}. Oxford: Oxford University Press, 2018, roč.~46, č.~4, s.~1624-1634. ISSN~0305-1048. Dostupné z: https://dx.doi.org/10.1093/nar/gky035.

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