Prussian blue nanoparticles as nanozyme label in immunoassays

a 2018

Prussian blue nanoparticles as nanozyme label in immunoassays

ČUNDERLOVÁ, Veronika, Antonín HLAVÁČEK, Zdeněk FARKA, Veronika HORÁČKOVÁ, Zuzana MIKUŠOVÁ et. al.

Základní údaje

Originální název

Prussian blue nanoparticles as nanozyme label in immunoassays

Autoři

ČUNDERLOVÁ, Veronika (203 Česká republika, domácí), Antonín HLAVÁČEK (203 Česká republika, domácí), Zdeněk FARKA (203 Česká republika, domácí), Veronika HORÁČKOVÁ (203 Česká republika, domácí), Zuzana MIKUŠOVÁ (703 Slovensko, domácí) a Petr SKLÁDAL (203 Česká republika, garant, domácí)

Vydání

CEITEC PhD and Postdoc Retreat, 2018

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10406 Analytical chemistry

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Kód RIV

RIV/00216224:14740/18:00102730

Organizační jednotka

Středoevropský technologický institut

ISBN

978-80-210-8941-9

Klíčová slova anglicky

Prussian blue nanoparticle; Enzyme biomimic; Bioconjugation; Sandwich immunoassay; Salmonella; Human serum albumin

Štítky

rivok

Změněno: 26. 3. 2019 15:18, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

Enzymes represent the most often used labels for amplification of signal in immunoassays due to their high catalytic efficiency. However, natural enzymes suffer from several disadvantages, e.g. they are easily denatured and their production and purification is generally complicated and time-consuming. Contrary, inorganic catalytic nanoparti-cles (nanozymes) offer easy and fast synthesis from commonly used chemicals. One of them are Prussian blue nanoparticles (PBNPs), which are nanozymes composed of coordination polymer of Fe4[Fe(CN)6]3. PBNPs contain the Fe3+/Fe2+ redox couple on the surface, which is also responsible for catalytic activity of peroxidases. PBNPs show high efficiency in catalyzing the oxidation of colorless 3,3,5,5-tetramethylbenzi-dine in the presence of H2O2 to form intensively blue product. We have prepared PBNPs with turnover number of 20 000 s-1 towards TMB oxidation, which is the highest value reported for nanoparticles of a size comparable to common proteins. We have also introduced the method for conjugation of PBNPs with antibody and utilization of the conjugate as a catalytic label in sandwich nanozyme-linked immunosorbent assay (NLISA). The practical applicability of NLISA was demonstrated by detection of different antigens, human serum albumin in urine and Salmonella Typhimurium in milk with limit of detection of 1.2 ng mL1 and 6·10^3 CFU mL1 respectively.

Návaznosti

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

ČUNDERLOVÁ, Veronika, Antonín HLAVÁČEK, Zdeněk FARKA, Veronika HORÁČKOVÁ, Zuzana MIKUŠOVÁ a Petr SKLÁDAL. Prussian blue nanoparticles as nanozyme label in immunoassays. In CEITEC PhD and Postdoc Retreat. 2018. ISBN 978-80-210-8941-9.

@proceedings{1417297,
   author = {Čunderlová, Veronika and Hlaváček, Antonín and Farka, Zdeněk and Horáčková, Veronika and Mikušová, Zuzana and Skládal, Petr},
   booktitle = {CEITEC PhD and Postdoc Retreat},
   keywords = {Prussian blue nanoparticle; Enzyme biomimic; Bioconjugation; Sandwich immunoassay; Salmonella; Human serum albumin},
   language = {eng},
   isbn = {978-80-210-8941-9},
   title = {Prussian blue nanoparticles as nanozyme label in immunoassays},
   url = {http://phdretreat.ceitec.cz/download/174},
   year = {2018}
}

TY  - CONF
ID  - 1417297
AU  - Čunderlová, Veronika - Hlaváček, Antonín - Farka, Zdeněk - Horáčková, Veronika - Mikušová, Zuzana - Skládal, Petr
PY  - 2018
TI  - Prussian blue nanoparticles as nanozyme label in immunoassays
SN  - 9788021089419
KW  - Prussian blue nanoparticle
KW  - Enzyme biomimic
KW  - Bioconjugation
KW  - Sandwich immunoassay
KW  - Salmonella
KW  - Human serum albumin
UR  - http://phdretreat.ceitec.cz/download/174
N2  - Enzymes represent the most often used labels for amplification of signal in immunoassays due to their high catalytic efficiency. However, natural enzymes suffer from several disadvantages, e.g. they are easily denatured and their production and purification is generally complicated and time-consuming. Contrary, inorganic catalytic nanoparti-cles (nanozymes) offer easy and fast synthesis from commonly used chemicals. One of them are Prussian blue nanoparticles (PBNPs), which are nanozymes composed of coordination polymer of Fe4[Fe(CN)6]3. PBNPs contain the Fe3+/Fe2+ redox couple on the surface, which is also responsible for catalytic activity of peroxidases. PBNPs show high efficiency in catalyzing the oxidation of colorless 3,3,5,5-tetramethylbenzi-dine in the presence of H2O2 to form intensively blue product. We have prepared PBNPs with turnover number of 20 000 s-1 towards TMB oxidation, which is the highest value reported for nanoparticles of a size comparable to common proteins. We have also introduced the method for conjugation of PBNPs with antibody and utilization of the conjugate as a catalytic label in sandwich nanozyme-linked immunosorbent assay (NLISA). The practical applicability of NLISA was demonstrated by detection of different antigens, human serum albumin in urine and Salmonella Typhimurium in milk with limit of detection of 1.2 ng mL1 and 6·10^3 CFU mL1 respectively.
ER  -

ČUNDERLOVÁ, Veronika, Antonín HLAVÁČEK, Zdeněk FARKA, Veronika HORÁČKOVÁ, Zuzana MIKUŠOVÁ a Petr SKLÁDAL. Prussian blue nanoparticles as nanozyme label in immunoassays. In \textit{CEITEC PhD and Postdoc Retreat}. 2018. ISBN~978-80-210-8941-9.

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