Survival of syngeneic and allogeneic iPSC–derived neural
precursors after spinal grafting in minipigs

J 2018

Survival of syngeneic and allogeneic iPSC–derived neural precursors after spinal grafting in minipigs

STRNADEL, Jan, Cassiano CARROMEU, Cedric BARDY, Michael NAVARRO, Oleksandr PLATOSHYN et. al.

Základní údaje

Originální název

Survival of syngeneic and allogeneic iPSC–derived neural precursors after spinal grafting in minipigs

Autoři

STRNADEL, Jan (840 Spojené státy), Cassiano CARROMEU (840 Spojené státy), Cedric BARDY (840 Spojené státy), Michael NAVARRO (840 Spojené státy), Oleksandr PLATOSHYN (840 Spojené státy), Andreas N. GLUD (840 Spojené státy), Silvia MARSALA (840 Spojené státy), Jozef KAFKA (840 Spojené státy), Atsushi MIYANOHARA (840 Spojené státy), Tomohisa KATO JR. (392 Japonsko), Takahiro TADOKORO (840 Spojené státy), Michael P. HEFFERAN (840 Spojené státy), Kota KAMIZATO (840 Spojené státy), Tetsuya YOSHIZUMI (840 Spojené státy), Stefan JUHAS (203 Česká republika), Jana JUHASOVA (203 Česká republika), Chak-Sum HO (840 Spojené státy), Taba KHERADMAND (840 Spojené státy), PeiXi CHEN (840 Spojené státy), Dáša BOHAČIAKOVÁ (703 Slovensko, domácí), Marian HRUSKA-PLOCHAN (840 Spojené státy), Andrew J. TODD (826 Velká Británie a Severní Irsko), Shawn P. DRISCOLL (840 Spojené státy), Thomas D. GLENN (840 Spojené státy), Samuel L. PFAFF (840 Spojené státy), Jiri KLIMA (203 Česká republika), Joseph CIACCI (840 Spojené státy), Eric CURTIS (840 Spojené státy), Fred H. GAGE (840 Spojené státy), Jack BUI (840 Spojené státy), Kazuhiko YAMADA (840 Spojené státy), Alysson R. MUOTRI (840 Spojené státy) a Martin MARSALA (840 Spojené státy, garant)

Vydání

Science Translational Medicine, Washington, American Association for the Advancement of Science, 2018, 1946-6234

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 17.200

Kód RIV

RIV/00216224:14110/18:00102732

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1126/scitranslmed.aam6651

UT WoS

000431766400001

Klíčová slova anglicky

cells derived; iPSC

Štítky

14110517, EL OK, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 10. 2. 2019 19:54, Soňa Böhmová

Anotace

V originále

The use of autologous (or syngeneic) cells derived from induced pluripotent stem cells (iPSCs) holds great promise for future clinical use in a wide range of diseases and injuries. It is expected that cell replacement therapies using autologous cells would forego the need for immunosuppression, otherwise required in allogeneic transplantations. However, recent studies have shown the unexpected immune rejection of undifferentiated autologous mouse iPSCs after transplantation. Whether similar immunogenic properties are maintained in iPSC-derived lineage-committed cells (such as neural precursors) is relatively unknown. We demonstrate that syngeneic porcine iPSC-derived neural precursor cell (NPC) transplantation to the spinal cord in the absence of immunosuppression is associated with long-term survival and neuronal and glial differentiation. No tumor formation was noted. Similar cell engraftment and differentiation were shown in spinally injured transiently immunosuppressed swine leukocyte antigen (SLA)–mismatched allogeneic pigs. These data demonstrate that iPSC-NPCs can be grafted into syngeneic recipients in the absence of immunosuppression and that temporary immunosuppression is sufficient to induce long-term immune tolerance after NPC engraftment into spinally injured allogeneic recipients. Collectively, our results show that iPSC-NPCs represent an alternative source of transplantable NPCs for the treatment of a variety of disorders affecting the spinal cord, including trauma, ischemia, or amyotrophic lateral sclerosis.

Citovat

STRNADEL, Jan, Cassiano CARROMEU, Cedric BARDY, Michael NAVARRO, Oleksandr PLATOSHYN, Andreas N. GLUD, Silvia MARSALA, Jozef KAFKA, Atsushi MIYANOHARA, Tomohisa KATO JR., Takahiro TADOKORO, Michael P. HEFFERAN, Kota KAMIZATO, Tetsuya YOSHIZUMI, Stefan JUHAS, Jana JUHASOVA, Chak-Sum HO, Taba KHERADMAND, PeiXi CHEN, Dáša BOHAČIAKOVÁ, Marian HRUSKA-PLOCHAN, Andrew J. TODD, Shawn P. DRISCOLL, Thomas D. GLENN, Samuel L. PFAFF, Jiri KLIMA, Joseph CIACCI, Eric CURTIS, Fred H. GAGE, Jack BUI, Kazuhiko YAMADA, Alysson R. MUOTRI a Martin MARSALA. Survival of syngeneic and allogeneic iPSC–derived neural precursors after spinal grafting in minipigs. Science Translational Medicine. Washington: American Association for the Advancement of Science, 2018, roč. 10, č. 440, s. 1-14. ISSN 1946-6234. Dostupné z: https://dx.doi.org/10.1126/scitranslmed.aam6651.

@article{1417346,
   author = {Strnadel, Jan and Carromeu, Cassiano and Bardy, Cedric and Navarro, Michael and Platoshyn, Oleksandr and Glud, Andreas N. and Marsala, Silvia and Kafka, Jozef and Miyanohara, Atsushi and Kato Jr., Tomohisa and Tadokoro, Takahiro and Hefferan, Michael P. and Kamizato, Kota and Yoshizumi, Tetsuya and Juhas, Stefan and Juhasova, Jana and Ho, ChakandSum and Kheradmand, Taba and Chen, PeiXi and Bohačiaková, Dáša and HruskaandPlochan, Marian and Todd, Andrew J. and Driscoll, Shawn P. and Glenn, Thomas D. and Pfaff, Samuel L. and Klima, Jiri and Ciacci, Joseph and Curtis, Eric and Gage, Fred H. and Bui, Jack and Yamada, Kazuhiko and Muotri, Alysson R. and Marsala, Martin},
   article_location = {Washington},
   article_number = {440},
   doi = {http://dx.doi.org/10.1126/scitranslmed.aam6651},
   keywords = {cells derived; iPSC},
   language = {eng},
   issn = {1946-6234},
   journal = {Science Translational Medicine},
   title = {Survival of syngeneic and allogeneic iPSC–derived neural precursors after spinal grafting in minipigs},
   url = {http://stm.sciencemag.org/content/10/440/eaam6651.short},
   volume = {10},
   year = {2018}
}

TY  - JOUR
ID  - 1417346
AU  - Strnadel, Jan - Carromeu, Cassiano - Bardy, Cedric - Navarro, Michael - Platoshyn, Oleksandr - Glud, Andreas N. - Marsala, Silvia - Kafka, Jozef - Miyanohara, Atsushi - Kato Jr., Tomohisa - Tadokoro, Takahiro - Hefferan, Michael P. - Kamizato, Kota - Yoshizumi, Tetsuya - Juhas, Stefan - Juhasova, Jana - Ho, Chak-Sum - Kheradmand, Taba - Chen, PeiXi - Bohačiaková, Dáša - Hruska-Plochan, Marian - Todd, Andrew J. - Driscoll, Shawn P. - Glenn, Thomas D. - Pfaff, Samuel L. - Klima, Jiri - Ciacci, Joseph - Curtis, Eric - Gage, Fred H. - Bui, Jack - Yamada, Kazuhiko - Muotri, Alysson R. - Marsala, Martin
PY  - 2018
TI  - Survival of syngeneic and allogeneic iPSC–derived neural precursors after spinal grafting in minipigs
JF  - Science Translational Medicine
VL  - 10
IS  - 440
SP  - 1-14
EP  - 1-14
PB  - American Association for the Advancement of Science
SN  - 19466234
KW  - cells derived
KW  - iPSC
UR  - http://stm.sciencemag.org/content/10/440/eaam6651.short
N2  - The use of autologous (or syngeneic) cells derived from induced pluripotent stem cells (iPSCs) holds great promise for future clinical use in a wide range of diseases and injuries. It is expected that cell replacement therapies using autologous cells would forego the need for immunosuppression, otherwise required in allogeneic transplantations. However, recent studies have shown the unexpected immune rejection of undifferentiated autologous mouse iPSCs after transplantation. Whether similar immunogenic properties are maintained in iPSC-derived lineage-committed cells (such as neural precursors) is relatively unknown. We demonstrate that syngeneic porcine iPSC-derived neural precursor cell (NPC) transplantation to the spinal cord in the absence of immunosuppression is associated with long-term survival and neuronal and glial differentiation. No tumor formation was noted. Similar cell engraftment and differentiation were shown in spinally injured transiently immunosuppressed swine leukocyte antigen (SLA)–mismatched allogeneic pigs. These data demonstrate that iPSC-NPCs can be grafted into syngeneic recipients in the absence of immunosuppression and that temporary immunosuppression is sufficient to induce long-term immune tolerance after NPC engraftment into spinally injured allogeneic recipients. Collectively, our results show that iPSC-NPCs represent an alternative source of transplantable NPCs for the treatment of a variety of disorders affecting the spinal cord, including trauma, ischemia, or amyotrophic lateral sclerosis.
ER  -

STRNADEL, Jan, Cassiano CARROMEU, Cedric BARDY, Michael NAVARRO, Oleksandr PLATOSHYN, Andreas N. GLUD, Silvia MARSALA, Jozef KAFKA, Atsushi MIYANOHARA, Tomohisa KATO JR., Takahiro TADOKORO, Michael P. HEFFERAN, Kota KAMIZATO, Tetsuya YOSHIZUMI, Stefan JUHAS, Jana JUHASOVA, Chak-Sum HO, Taba KHERADMAND, PeiXi CHEN, Dáša BOHAČIAKOVÁ, Marian HRUSKA-PLOCHAN, Andrew J. TODD, Shawn P. DRISCOLL, Thomas D. GLENN, Samuel L. PFAFF, Jiri KLIMA, Joseph CIACCI, Eric CURTIS, Fred H. GAGE, Jack BUI, Kazuhiko YAMADA, Alysson R. MUOTRI a Martin MARSALA. Survival of syngeneic and allogeneic iPSC–derived neural precursors after spinal grafting in minipigs. \textit{Science Translational Medicine}. Washington: American Association for the Advancement of Science, 2018, roč.~10, č.~440, s.~1-14. ISSN~1946-6234. Dostupné z: https://dx.doi.org/10.1126/scitranslmed.aam6651.

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