A method for analysis of marker persistent organic pollutants
in low-volume plasma and serum samples using 96-well plate
solid phase extraction

J 2018

A method for analysis of marker persistent organic pollutants in low-volume plasma and serum samples using 96-well plate solid phase extraction

STUBLESKI, Jordan, Petr KUKUČKA, Samira SALIHOVIC, P. Monica LIND, Lars LIND et. al.

Základní údaje

Originální název

A method for analysis of marker persistent organic pollutants in low-volume plasma and serum samples using 96-well plate solid phase extraction

Autoři

STUBLESKI, Jordan (752 Švédsko), Petr KUKUČKA (203 Česká republika, garant, domácí), Samira SALIHOVIC (752 Švédsko), P. Monica LIND (752 Švédsko), Lars LIND (752 Švédsko) a Anna KARRMAN

Vydání

Journal of Chromatography A, Amsterdam, Netherlands, Elsevier, 2018, 0021-9673

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.858

Kód RIV

RIV/00216224:14310/18:00102902

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.chroma.2018.02.057

UT WoS

000430766800003

Klíčová slova anglicky

High-throughput SPE; Persistent organic pollutants; GC-HRMS; APCI-MS/MS

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 3. 6. 2018 12:06, Mgr. Michaela Hylsová, Ph.D.

Anotace

V originále

The objective of this study was to develop and validate a 96-well plate solid phase extraction method for analysis of 23 lipophilic persistent organic pollutants (POPs) in low-volume plasma and serum samples which is applicable for biomonitoring and epidemiological studies. The analysis of selected markers for internal exposure: 16 polychlorinated biphenyls (PCBs), 5 organochlorine pesticides (OCPs), octachlorinated dibenzo-p-dioxin (OCDD), and polybrominated diphenylether 47 (BDE 47) was evaluated by comparing two SPE sorbents and GC-HRMS or GC-MS/MS detection. The final method extracted 23 POPs from 150 mu L of serum and plasma using a 96-well extraction plate containing 60 mg Oasis HLB sorbent per well prior to GC-HRMS magnetic sector analysis. The extraction method was applied to 40 plasma samples collected for an epidemiological study. The recovery of selected POPs ranged from 31% to 63% (n = 48), and detection limits ranged from 2.2 to 45 pg/mL for PCBs, 4.2 to 167 pg/mL for OCPs, 7.8 pg/mL for OCDD and 6.1 pg/mL for BDE 47. This method showed good precision with relative standard deviations of selected POP concentrations in quality control samples (n = 48) ranging from 11% to 25%. The trueness was determined with standard reference material serum (n = 48) and the deviation from certified values ranged from 1 to 27%. Of the 23 POPs analyzed, 18 were detected in 43% to 100% of plasma samples collected for the epidemiological study. The method showed good robustness with low inter-well plate variation (11-31%) determined by twelve 96-well plate extractions, and can extract 96 samples, including quality controls and procedural blanks in 2-3 days. Comparison with GC-MS/MS analysis showed that similar concentrations (within 0.5% to 30%) of most POPs could be obtained with GC-APCI-MS/MS. Larger deviations were observed for PCB 194 (60%) and trans-nonachlor (43%). The developed method produces accurate concentrations of low-level marker POPs in plasma and serum, providing a suitable high-throughput sample preparation procedure for biomonitoring and epidemiological studies involving large sample size and limited sample volume. GC-HRMS was chosen over GC-MS/MS, however the latter showed promising results, and could be used as an alternative to GC-HRMS analysis for most POPs.

Návaznosti

CZ.02.1.01/0.0/0.0/16_013/0001761, interní kód MU

Název: RECETOX RI - OP VVV (Akronym: RECETOX RI)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, RECETOX RI - OP VVV, PO 1 Posilování kapacit pro kvalitní výzkum

LM2015051, projekt VaV

Název: Centrum pro výzkum toxických látek v prostředí (Akronym: RECETOX RI)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Výzkumná infrastruktura RECETOX

Citovat

STUBLESKI, Jordan, Petr KUKUČKA, Samira SALIHOVIC, P. Monica LIND, Lars LIND a Anna KARRMAN. A method for analysis of marker persistent organic pollutants in low-volume plasma and serum samples using 96-well plate solid phase extraction. Journal of Chromatography A. Amsterdam, Netherlands: Elsevier, 2018, roč. 1546, April, s. 18-27. ISSN 0021-9673. Dostupné z: https://dx.doi.org/10.1016/j.chroma.2018.02.057.

@article{1419440,
   author = {Stubleski, Jordan and Kukučka, Petr and Salihovic, Samira and Lind, P. Monica and Lind, Lars and Karrman, Anna},
   article_location = {Amsterdam, Netherlands},
   article_number = {April},
   doi = {http://dx.doi.org/10.1016/j.chroma.2018.02.057},
   keywords = {High-throughput SPE; Persistent organic pollutants; GC-HRMS; APCI-MS/MS},
   language = {eng},
   issn = {0021-9673},
   journal = {Journal of Chromatography A},
   title = {A method for analysis of marker persistent organic pollutants in low-volume plasma and serum samples using 96-well plate solid phase extraction},
   url = {https://www.sciencedirect.com/science/article/pii/S002196731830253X?via%3Dihub},
   volume = {1546},
   year = {2018}
}

TY  - JOUR
ID  - 1419440
AU  - Stubleski, Jordan - Kukučka, Petr - Salihovic, Samira - Lind, P. Monica - Lind, Lars - Karrman, Anna
PY  - 2018
TI  - A method for analysis of marker persistent organic pollutants in low-volume plasma and serum samples using 96-well plate solid phase extraction
JF  - Journal of Chromatography A
VL  - 1546
IS  - April
SP  - 18-27
EP  - 18-27
PB  - Elsevier
SN  - 00219673
KW  - High-throughput SPE
KW  - Persistent organic pollutants
KW  - GC-HRMS
KW  - APCI-MS/MS
UR  - https://www.sciencedirect.com/science/article/pii/S002196731830253X?via%3Dihub
L2  - https://www.sciencedirect.com/science/article/pii/S002196731830253X?via%3Dihub
N2  - The objective of this study was to develop and validate a 96-well plate solid phase extraction method for analysis of 23 lipophilic persistent organic pollutants (POPs) in low-volume plasma and serum samples which is applicable for biomonitoring and epidemiological studies. The analysis of selected markers for internal exposure: 16 polychlorinated biphenyls (PCBs), 5 organochlorine pesticides (OCPs), octachlorinated dibenzo-p-dioxin (OCDD), and polybrominated diphenylether 47 (BDE 47) was evaluated by comparing two SPE sorbents and GC-HRMS or GC-MS/MS detection. The final method extracted 23 POPs from 150 mu L of serum and plasma using a 96-well extraction plate containing 60 mg Oasis HLB sorbent per well prior to GC-HRMS magnetic sector analysis. The extraction method was applied to 40 plasma samples collected for an epidemiological study. The recovery of selected POPs ranged from 31% to 63% (n = 48), and detection limits ranged from 2.2 to 45 pg/mL for PCBs, 4.2 to 167 pg/mL for OCPs, 7.8 pg/mL for OCDD and 6.1 pg/mL for BDE 47. This method showed good precision with relative standard deviations of selected POP concentrations in quality control samples (n = 48) ranging from 11% to 25%. The trueness was determined with standard reference material serum (n = 48) and the deviation from certified values ranged from 1 to 27%. Of the 23 POPs analyzed, 18 were detected in 43% to 100% of plasma samples collected for the epidemiological study. The method showed good robustness with low inter-well plate variation (11-31%) determined by twelve 96-well plate extractions, and can extract 96 samples, including quality controls and procedural blanks in 2-3 days. Comparison with GC-MS/MS analysis showed that similar concentrations (within 0.5% to 30%) of most POPs could be obtained with GC-APCI-MS/MS. Larger deviations were observed for PCB 194 (60%) and trans-nonachlor (43%). The developed method produces accurate concentrations of low-level marker POPs in plasma and serum, providing a suitable high-throughput sample preparation procedure for biomonitoring and epidemiological studies involving large sample size and limited sample volume. GC-HRMS was chosen over GC-MS/MS, however the latter showed promising results, and could be used as an alternative to GC-HRMS analysis for most POPs.
ER  -

STUBLESKI, Jordan, Petr KUKUČKA, Samira SALIHOVIC, P. Monica LIND, Lars LIND a Anna KARRMAN. A method for analysis of marker persistent organic pollutants in low-volume plasma and serum samples using 96-well plate solid phase extraction. \textit{Journal of Chromatography A}. Amsterdam, Netherlands: Elsevier, 2018, roč.~1546, April, s.~18-27. ISSN~0021-9673. Dostupné z: https://dx.doi.org/10.1016/j.chroma.2018.02.057.

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