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@article{1419981,
author = {Celichowski, Piotr and Nawrocki, Mariusz J. and DyszkiewiczandKonwinska, Marta and Jankowski, Maurycy and Budna, Joanna and Bryja, Artur and Kranc, Wieslawa and Borys, Sylwia and Knap, Sandra and Ciesiolka, Sylwia and Ješeta, Michal and PiaseckaandStryczynska, Karolina and Khozmi, Ronza and Bukowska, Dorota and Antosik, Pawel and Brussow, Klaus P. and Bruska, Malgorzata and Nowicki, Michal and Zabel, Maciej and Kempisty, Bartosz},
article_location = {New York},
article_number = {2863068},
doi = {http://dx.doi.org/10.1155/2018/2863068},
keywords = {RNA Metabolic Process},
language = {eng},
issn = {2314-6133},
journal = {Biomed Research International},
title = {"Positive Regulation of RNA Metabolic Process" Ontology Group Highly Regulated in Porcine Oocytes Matured In Vitro: A Microarray Approach},
volume = {2018},
year = {2018}
}
TY - JOUR
ID - 1419981
AU - Celichowski, Piotr - Nawrocki, Mariusz J. - Dyszkiewicz-Konwinska, Marta - Jankowski, Maurycy - Budna, Joanna - Bryja, Artur - Kranc, Wieslawa - Borys, Sylwia - Knap, Sandra - Ciesiolka, Sylwia - Ješeta, Michal - Piasecka-Stryczynska, Karolina - Khozmi, Ronza - Bukowska, Dorota - Antosik, Pawel - Brussow, Klaus P. - Bruska, Malgorzata - Nowicki, Michal - Zabel, Maciej - Kempisty, Bartosz
PY - 2018
TI - "Positive Regulation of RNA Metabolic Process" Ontology Group Highly Regulated in Porcine Oocytes Matured In Vitro: A Microarray Approach
JF - Biomed Research International
VL - 2018
IS - 2863068
SP - 1-10
EP - 1-10
PB - Hindawi Publishing Corporation
SN - 23146133
KW - RNA Metabolic Process
N2 - The cumulus-oocyte complexes (COCs) growth and development during folliculogenesis and oogenesis are accompanied by changes involving synthesis and accumulation of large amount of RNA and proteins. In this study, the transcriptomic profile of genes involved in "oocytes RNA synthesis" in relation to in vitro maturation in pigs was investigated for the first time. The RNA was isolated from oocytes before and after in vitro maturation (IVM). Interactions between differentially expressed genes/ proteins belonging to "positive regulation of RNA metabolic process" ontology group were investigated by STRING10 software. Using microarray assays, we found expression of 12258 porcine transcripts. Genes with fold change higher than |2| and with corrected.. value lower than 0.05 were considered as differentially expressed. The ontology group "positive regulation of RNA metabolic process" involved differential expression of AR, INHBA, WWTR1, FOS, MEF2C, VEGFA, IKZF2, IHH, RORA, MAP3K1, NFAT5, SMARCA1, EGR1, EGR2, MITF, SMAD4, APP, and NR5A1 transcripts. Since all of the presented genes were downregulated after IVM, we suggested that they might be significantly involved in regulation of RNA synthesis before reaching oocyte MII stage. Higher expression of "RNA metabolic process" related genes before IVM indicated that they might be recognized as important markers and specific "transcriptomic fingerprint" of RNA template accumulation and storage for further porcine embryos growth and development.
ER -
CELICHOWSKI, Piotr, Mariusz J. NAWROCKI, Marta DYSZKIEWICZ-KONWINSKA, Maurycy JANKOWSKI, Joanna BUDNA, Artur BRYJA, Wieslawa KRANC, Sylwia BORYS, Sandra KNAP, Sylwia CIESIOLKA, Michal JEŠETA, Karolina PIASECKA-STRYCZYNSKA, Ronza KHOZMI, Dorota BUKOWSKA, Pawel ANTOSIK, Klaus P. BRUSSOW, Malgorzata BRUSKA, Michal NOWICKI, Maciej ZABEL a Bartosz KEMPISTY. ''Positive Regulation of RNA Metabolic Process'' Ontology Group Highly Regulated in Porcine Oocytes Matured In Vitro: A Microarray Approach. \textit{Biomed Research International}. New York: Hindawi Publishing Corporation, 2018, roč.~2018, č.~2863068, s.~1-10. ISSN~2314-6133. Dostupné z: https://dx.doi.org/10.1155/2018/2863068.
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