J 2018

"Positive Regulation of RNA Metabolic Process" Ontology Group Highly Regulated in Porcine Oocytes Matured In Vitro: A Microarray Approach

CELICHOWSKI, Piotr, Mariusz J. NAWROCKI, Marta DYSZKIEWICZ-KONWINSKA, Maurycy JANKOWSKI, Joanna BUDNA et. al.

Základní údaje

Originální název

"Positive Regulation of RNA Metabolic Process" Ontology Group Highly Regulated in Porcine Oocytes Matured In Vitro: A Microarray Approach

Autoři

CELICHOWSKI, Piotr (616 Polsko), Mariusz J. NAWROCKI (616 Polsko), Marta DYSZKIEWICZ-KONWINSKA (616 Polsko), Maurycy JANKOWSKI (616 Polsko), Joanna BUDNA (616 Polsko), Artur BRYJA (616 Polsko), Wieslawa KRANC (616 Polsko), Sylwia BORYS (616 Polsko), Sandra KNAP (616 Polsko), Sylwia CIESIOLKA (616 Polsko), Michal JEŠETA (203 Česká republika, domácí), Karolina PIASECKA-STRYCZYNSKA (616 Polsko), Ronza KHOZMI (616 Polsko), Dorota BUKOWSKA (616 Polsko), Pawel ANTOSIK (616 Polsko), Klaus P. BRUSSOW (616 Polsko), Malgorzata BRUSKA (616 Polsko), Michal NOWICKI (616 Polsko), Maciej ZABEL (616 Polsko) a Bartosz KEMPISTY (616 Polsko, garant)

Vydání

Biomed Research International, New York, Hindawi Publishing Corporation, 2018, 2314-6133

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30401 Health-related biotechnology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.197

Kód RIV

RIV/00216224:14110/18:00102953

Organizační jednotka

Lékařská fakulta

UT WoS

000419696500001

Klíčová slova anglicky

RNA Metabolic Process

Štítky

Příznaky

Mezinárodní význam, Recenzováno
Změněno: 10. 2. 2019 17:32, Soňa Böhmová

Anotace

V originále

The cumulus-oocyte complexes (COCs) growth and development during folliculogenesis and oogenesis are accompanied by changes involving synthesis and accumulation of large amount of RNA and proteins. In this study, the transcriptomic profile of genes involved in "oocytes RNA synthesis" in relation to in vitro maturation in pigs was investigated for the first time. The RNA was isolated from oocytes before and after in vitro maturation (IVM). Interactions between differentially expressed genes/ proteins belonging to "positive regulation of RNA metabolic process" ontology group were investigated by STRING10 software. Using microarray assays, we found expression of 12258 porcine transcripts. Genes with fold change higher than |2| and with corrected.. value lower than 0.05 were considered as differentially expressed. The ontology group "positive regulation of RNA metabolic process" involved differential expression of AR, INHBA, WWTR1, FOS, MEF2C, VEGFA, IKZF2, IHH, RORA, MAP3K1, NFAT5, SMARCA1, EGR1, EGR2, MITF, SMAD4, APP, and NR5A1 transcripts. Since all of the presented genes were downregulated after IVM, we suggested that they might be significantly involved in regulation of RNA synthesis before reaching oocyte MII stage. Higher expression of "RNA metabolic process" related genes before IVM indicated that they might be recognized as important markers and specific "transcriptomic fingerprint" of RNA template accumulation and storage for further porcine embryos growth and development.