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@article{1422248,
author = {Trnková, Andrea and Šancová, Kateřina and Zapletalová, Martina and Kašparovská, Jitka and Dadáková, Kateřina and Křížová, Ludmila and Lochman, Jan and Hadrová, Sylvie and Ihnatová, Ivana and Kašparovský, Tomáš},
article_location = {New York},
article_number = {6},
doi = {http://dx.doi.org/10.3168/jds.2017-13610},
keywords = {isoflavones; equol; rumen; cattle diet},
language = {eng},
issn = {0022-0302},
journal = {Journal of Dairy Science},
title = {Determination of in vitro isoflavone degradation in rumen fluid},
url = {http://dx.doi.org/10.3168/jds.2017-13610},
volume = {101},
year = {2018}
}
TY - JOUR
ID - 1422248
AU - Trnková, Andrea - Šancová, Kateřina - Zapletalová, Martina - Kašparovská, Jitka - Dadáková, Kateřina - Křížová, Ludmila - Lochman, Jan - Hadrová, Sylvie - Ihnatová, Ivana - Kašparovský, Tomáš
PY - 2018
TI - Determination of in vitro isoflavone degradation in rumen fluid
JF - Journal of Dairy Science
VL - 101
IS - 6
SP - 5134-5144
EP - 5134-5144
PB - Elsevier Science Inc.
SN - 00220302
KW - isoflavones
KW - equol
KW - rumen
KW - cattle diet
UR - http://dx.doi.org/10.3168/jds.2017-13610
N2 - The aim of this study was to determine the degradation of dietary isoflavones in rumen fluid under 2 feeding regimens. The experiments were performed in vitro using a rumen fluid buffer system. The rumen fluid was taken from cows fed either a hay diet or a concentrate-rich diet (the diet consisted of 34.6% maize silage, 17.6% haylage, 12.8% alfalfa hay, and 35.0% supplemental mixture on a dry matter basis). As a source of isoflavones, 40% soybean extract (Biomedica, Prague, Czech Republic) at levels of 5, 25, 50, and 75 mg per 40 mL of rumen fluid was used. Samples of soybean extract were incubated in triplicate at 39 degrees C for 0, 3.0, 6.0, 12.0, arid 24.0 h in incubation solution. The metabolism of daidzein and genistein was faster under concentrate-rich diet conditions. In general, production of equol started after 3 to 6 h of incubation and reached the highest rate after approximately 12 h of incubation regardless of the type of diet or concentration of extract. In most of the experiments, production of equol continued after 24 h of incubation. Generally, equol production was greater under the hay diet conditions. Furthermore, experiments with higher amounts of added soybean extract revealed possible inhibitory effects of high levels of isoflavones on the rumen microflora.
ER -
TRNKOVÁ, Andrea, Kateřina ŠANCOVÁ, Martina ZAPLETALOVÁ, Jitka KAŠPAROVSKÁ, Kateřina DADÁKOVÁ, Ludmila KŘÍŽOVÁ, Jan LOCHMAN, Sylvie HADROVÁ, Ivana IHNATOVÁ and Tomáš KAŠPAROVSKÝ. Determination of in vitro isoflavone degradation in rumen fluid. \textit{Journal of Dairy Science}. New York: Elsevier Science Inc., 2018, vol.~101, No~6, p.~5134-5144. ISSN~0022-0302. Available from: https://dx.doi.org/10.3168/jds.2017-13610.
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