Laser microdissection and mass spectrometry: protein analysis
of strictly selected tissue from Eudiplozoon nipponicum

k 2018

Laser microdissection and mass spectrometry: protein analysis of strictly selected tissue from Eudiplozoon nipponicum

ROUDNICKÝ, Pavel, Tomáš LOJA, David POTĚŠIL, Zbyněk ZDRÁHAL, Milan GELNAR et. al.

Základní údaje

Originální název

Laser microdissection and mass spectrometry: protein analysis of strictly selected tissue from Eudiplozoon nipponicum

Autoři

ROUDNICKÝ, Pavel (203 Česká republika, garant, domácí), Tomáš LOJA (703 Slovensko, domácí), David POTĚŠIL (203 Česká republika, domácí), Zbyněk ZDRÁHAL (203 Česká republika, domácí), Milan GELNAR (203 Česká republika, domácí) a Martin KAŠNÝ (203 Česká republika, domácí)

Vydání

24th Helminthological Days, 2018

Další údaje

Jazyk

angličtina

Typ výsledku

Prezentace na konferencích

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

web konference

Kód RIV

RIV/00216224:14310/18:00101088

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-7444-057-1

Klíčová slova anglicky

Monogenea; parasite; platyhelminth; laser microdissection; mass spectrometry; protein

Změněno: 31. 7. 2018 13:44, Mgr. Pavel Roudnický, Ph.D.

Anotace

V originále

Laser microdissection is a combined microscopic-separation technique enabling to extract the samples of interest from histological slides - single cells, organs and tissues. The isolated samples can be further analysed and various parameters determined. The adoption of mass spectrometry analysis could give us the detail information about the sample composition and relative quantity of particular components (e.g. protein molecules) can be calculated too. Moreover, this technology enables the fast determination of distribution of selected molecule within the studied biological object. Thus, we can avoid the time-consuming preparation of specific antibodies in laboratory animals, which are widely used for protein tissue localization. In our current research, we used Eudiplozoon nipponicum, ectoparasitic blood-feeder from gills of Cyprinus carpio with aim to reveal the protein composition of its tegument region, gut and inner parenchyma. So far, we have identified 405 unique proteins in inner parenchyma/gut and 131 unique proteins in tegument. Among them, some already described functional molecules have been found.

Návaznosti

GBP505/12/G112, projekt VaV

Název: ECIP - Evropské centrum ichtyoparazitologie

Investor: Grantová agentura ČR, ECIP - Evropské centrum ichtyoparazitologie

MUNI/A/0816/2017, interní kód MU

Název: Výzkum ekologicko-evolučních vztahů bezobratlých živočichů (Akronym: EKOLINKS)

Investor: Masarykova univerzita, Výzkum ekologicko-evolučních vztahů bezobratlých živočichů, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Citovat

ROUDNICKÝ, Pavel, Tomáš LOJA, David POTĚŠIL, Zbyněk ZDRÁHAL, Milan GELNAR a Martin KAŠNÝ. Laser microdissection and mass spectrometry: protein analysis of strictly selected tissue from Eudiplozoon nipponicum. In 24th Helminthological Days. 2018. ISBN 978-80-7444-057-1.

@proceedings{1425516,
   author = {Roudnický, Pavel and Loja, Tomáš and Potěšil, David and Zdráhal, Zbyněk and Gelnar, Milan and Kašný, Martin},
   booktitle = {24th Helminthological Days},
   keywords = {Monogenea; parasite; platyhelminth; laser microdissection; mass spectrometry; protein},
   language = {eng},
   isbn = {978-80-7444-057-1},
   title = {Laser microdissection and mass spectrometry: protein analysis of strictly selected tissue from Eudiplozoon nipponicum},
   url = {https://hd2018.natur.cuni.cz/},
   year = {2018}
}

TY  - CONF
ID  - 1425516
AU  - Roudnický, Pavel - Loja, Tomáš - Potěšil, David - Zdráhal, Zbyněk - Gelnar, Milan - Kašný, Martin
PY  - 2018
TI  - Laser microdissection and mass spectrometry: protein analysis of strictly selected tissue from Eudiplozoon nipponicum
SN  - 9788074440571
KW  - Monogenea
KW  - parasite
KW  - platyhelminth
KW  - laser microdissection
KW  - mass spectrometry
KW  - protein
UR  - https://hd2018.natur.cuni.cz/
L2  - https://hd2018.natur.cuni.cz/
N2  - Laser microdissection is a combined microscopic-separation technique enabling to extract the samples of interest from histological slides - single cells, organs and tissues. The isolated samples can be further analysed and various parameters determined. The adoption of mass spectrometry analysis could give us the detail information about the sample composition and relative quantity of particular components (e.g. protein molecules) can be calculated too. Moreover, this technology enables the fast determination of distribution of selected molecule within the studied biological object. Thus, we can avoid the time-consuming preparation of specific antibodies in laboratory animals, which are widely used for protein tissue localization. In our current research, we used Eudiplozoon nipponicum, ectoparasitic blood-feeder from gills of Cyprinus carpio with aim to reveal the protein composition of its tegument region, gut and inner parenchyma. So far, we have identified 405 unique proteins in inner parenchyma/gut and 131 unique proteins in tegument. Among them, some already described functional molecules have been found.
ER  -

ROUDNICKÝ, Pavel, Tomáš LOJA, David POTĚŠIL, Zbyněk ZDRÁHAL, Milan GELNAR a Martin KAŠNÝ. Laser microdissection and mass spectrometry: protein analysis of strictly selected tissue from Eudiplozoon nipponicum. In \textit{24th Helminthological Days}. 2018. ISBN~978-80-7444-057-1.

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