Functionally specific binding regions of microtubule-associated
protein 2c exhibit distinct conformations and dynamics

J 2018

Functionally specific binding regions of microtubule-associated protein 2c exhibit distinct conformations and dynamics

MELKOVÁ, Kateřina, Vojtěch ZAPLETAL, Séverine JANSEN, Erik NOMILNER, Milan ZACHRDLA et. al.

Basic information

Original name

Functionally specific binding regions of microtubule-associated protein 2c exhibit distinct conformations and dynamics

Authors

MELKOVÁ, Kateřina (203 Czech Republic, belonging to the institution), Vojtěch ZAPLETAL (203 Czech Republic, belonging to the institution), Séverine JANSEN (250 France, belonging to the institution), Erik NOMILNER (703 Slovakia, belonging to the institution), Milan ZACHRDLA (203 Czech Republic, belonging to the institution), Jozef HRITZ (703 Slovakia, belonging to the institution), Jiří NOVÁČEK (203 Czech Republic, belonging to the institution), M. ZWECKSTETTER (276 Germany), M.R. JENSEN (250 France), M. BLACKLEDGE (250 France) and Lukáš ŽÍDEK (203 Czech Republic, guarantor, belonging to the institution)

Edition

Journal of Biological Chemistry, Bethesda, USA, Amer. Soc. Biochem. Mol. Biol. 2018, 0021-9258

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.106

RIV identification code

RIV/00216224:14740/18:00101098

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1074/jbc.RA118.001769

UT WoS

000442730200027

Keywords in English

NMR relaxation; Tau protein (Tau); microtubule-associated protein (MAP); nuclear magnetic resonance (NMR); paramagnetic relaxation enhancement (PRE); protein conformation; small-angle X-ray scattering (SAXS)

Abstract

V originále

Microtubule-associated protein 2c (MAP2c) is a 49-kDa intrinsically disordered protein regulating the dynamics of microtubules in developing neurons. MAP2c differs from its sequence homologue Tau in the pattern and kinetics of phosphorylation by cAMP-dependent protein kinase (PKA). Moreover, the mechanisms through which MAP2c interacts with its binding partners and the conformational changes and dynamics associated with these interactions remain unclear. Here, we used NMR relaxation and paramagnetic relaxation enhancement techniques to determine the dynamics and long-range interactions within MAP2c. The relaxation rates revealed large differences in flexibility of individual regions of MAP2c, with the lowest flexibility observed in the known and proposed binding sites. Quantitative conformational analyses of chemical shifts, small-angle X-ray scattering (SAXS), and paramagnetic relaxation enhancement measurements disclosed that MAP2c regions interacting with important protein partners, including Fyn tyrosine kinase, plectin, and PKA, adopt specific conformations. High populations of polyproline II and alpha-helices were found in Fyn- and plectin-binding sites of MAP2c, respectively. The region binding the regulatory subunit of PKA consists of two helical motifs bridged by a more extended conformation. Of note, although MAP2c and Tau did not differ substantially in their conformations in regions of high sequence identity, we found that they differ significantly in long-range interactions, dynamics, and local conformation motifs in their N-terminal domains. These results highlight that the N-terminal regions of MAP2c provide important specificity to its regulatory roles and indicate a close relationship between MAP2c's biological functions and conformational behavior.

Links

EF16_013/0001776, research and development project

Name: Česká infrastruktura pro integrativní strukturní biologii pro lidské zdraví

GA15-14974S, research and development project

Name: Charakterizace proteinu MAP2c (microtubule associated protein 2c) a modifikací regulujících jeho funkci s atomovým rozlišením

Investor: Czech Science Foundation

LM2015043, research and development project

Name: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)

Investor: Ministry of Education, Youth and Sports of the CR

LM2015085, research and development project

Name: CERIT Scientific Cloud (Acronym: CERIT-SC)

Investor: Ministry of Education, Youth and Sports of the CR, CERIT Scientific Cloud

692068, interní kód MU

Name: BISON - Bridging Structural Biology with Biological Synthesis and Self Assembly to Reveal Key Processes in Living Systems (Acronym: BISON)

Investor: European Union, Spreading excellence and widening participation

Files attached

melkova-revised.pdf

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Citovat

MELKOVÁ, Kateřina, Vojtěch ZAPLETAL, Séverine JANSEN, Erik NOMILNER, Milan ZACHRDLA, Jozef HRITZ, Jiří NOVÁČEK, M. ZWECKSTETTER, M.R. JENSEN, M. BLACKLEDGE and Lukáš ŽÍDEK. Functionally specific binding regions of microtubule-associated protein 2c exhibit distinct conformations and dynamics. Journal of Biological Chemistry. Bethesda, USA: Amer. Soc. Biochem. Mol. Biol., 2018, vol. 293, No 34, p. 13297-13309. ISSN 0021-9258. Available from: https://dx.doi.org/10.1074/jbc.RA118.001769.

@article{1427578,
   author = {Melková, Kateřina and Zapletal, Vojtěch and Jansen, Séverine and Nomilner, Erik and Zachrdla, Milan and Hritz, Jozef and Nováček, Jiří and Zweckstetter, M. and Jensen, M.R. and Blackledge, M. and Žídek, Lukáš},
   article_location = {Bethesda, USA},
   article_number = {34},
   doi = {http://dx.doi.org/10.1074/jbc.RA118.001769},
   keywords = {NMR relaxation; Tau protein (Tau); microtubule-associated protein (MAP); nuclear magnetic resonance (NMR); paramagnetic relaxation enhancement (PRE); protein conformation; small-angle X-ray scattering (SAXS)},
   language = {eng},
   issn = {0021-9258},
   journal = {Journal of Biological Chemistry},
   title = {Functionally specific binding regions of microtubule-associated protein 2c exhibit distinct conformations and dynamics},
   url = {http://www.jbc.org/content/early/2018/06/20/jbc.RA118.001769.abstract},
   volume = {293},
   year = {2018}
}

TY  - JOUR
ID  - 1427578
AU  - Melková, Kateřina - Zapletal, Vojtěch - Jansen, Séverine - Nomilner, Erik - Zachrdla, Milan - Hritz, Jozef - Nováček, Jiří - Zweckstetter, M. - Jensen, M.R. - Blackledge, M. - Žídek, Lukáš
PY  - 2018
TI  - Functionally specific binding regions of microtubule-associated protein 2c exhibit distinct conformations and dynamics
JF  - Journal of Biological Chemistry
VL  - 293
IS  - 34
SP  - 13297-13309
EP  - 13297-13309
PB  - Amer. Soc. Biochem. Mol. Biol.
SN  - 00219258
KW  - NMR relaxation
KW  - Tau protein (Tau)
KW  - microtubule-associated protein (MAP)
KW  - nuclear magnetic resonance (NMR)
KW  - paramagnetic relaxation enhancement (PRE)
KW  - protein conformation
KW  - small-angle X-ray scattering (SAXS)
UR  - http://www.jbc.org/content/early/2018/06/20/jbc.RA118.001769.abstract
N2  - Microtubule-associated protein 2c (MAP2c) is a 49-kDa intrinsically disordered protein regulating the dynamics of microtubules in developing neurons. MAP2c differs from its sequence homologue Tau in the pattern and kinetics of phosphorylation by cAMP-dependent protein kinase (PKA). Moreover, the mechanisms through which MAP2c interacts with its binding partners and the conformational changes and dynamics associated with these interactions remain unclear. Here, we used NMR relaxation and paramagnetic relaxation enhancement techniques to determine the dynamics and long-range interactions within MAP2c. The relaxation rates revealed large differences in flexibility of individual regions of MAP2c, with the lowest flexibility observed in the known and proposed binding sites. Quantitative conformational analyses of chemical shifts, small-angle X-ray scattering (SAXS), and paramagnetic relaxation enhancement measurements disclosed that MAP2c regions interacting with important protein partners, including Fyn tyrosine kinase, plectin, and PKA, adopt specific conformations. High populations of polyproline II and alpha-helices were found in Fyn- and plectin-binding sites of MAP2c, respectively. The region binding the regulatory subunit of PKA consists of two helical motifs bridged by a more extended conformation. Of note, although MAP2c and Tau did not differ substantially in their conformations in regions of high sequence identity, we found that they differ significantly in long-range interactions, dynamics, and local conformation motifs in their N-terminal domains. These results highlight that the N-terminal regions of MAP2c provide important specificity to its regulatory roles and indicate a close relationship between MAP2c's biological functions and conformational behavior.
ER  -

MELKOVÁ, Kateřina, Vojtěch ZAPLETAL, Séverine JANSEN, Erik NOMILNER, Milan ZACHRDLA, Jozef HRITZ, Jiří NOVÁČEK, M. ZWECKSTETTER, M.R. JENSEN, M. BLACKLEDGE and Lukáš ŽÍDEK. Functionally specific binding regions of microtubule-associated protein 2c exhibit distinct conformations and dynamics. \textit{Journal of Biological Chemistry}. Bethesda, USA: Amer. Soc. Biochem. Mol. Biol., 2018, vol.~293, No~34, p.~13297-13309. ISSN~0021-9258. Available from: https://dx.doi.org/10.1074/jbc.RA118.001769.

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