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@article{1429256,
author = {Celá, Andrea and Mádr, Aleš and Ješeta, Michal and Žáková, Jana and Crha, Igor and Glatz, Zdeněk},
article_location = {Weinheim},
article_number = {23},
doi = {http://dx.doi.org/10.1002/elps.201800265},
keywords = {Amino acids; Capillary electrophoresis with light emitting diode induced fluorescence detection;},
language = {eng},
issn = {0173-0835},
journal = {Electrophoresis},
title = {Study of metabolic activity of human embryos focused on amino acids by capillary electrophoresis with light-emitting diode-induced fluorescence detection},
url = {http://dx.doi.org/10.1002/elps.201800265},
volume = {39},
year = {2018}
}
TY - JOUR
ID - 1429256
AU - Celá, Andrea - Mádr, Aleš - Ješeta, Michal - Žáková, Jana - Crha, Igor - Glatz, Zdeněk
PY - 2018
TI - Study of metabolic activity of human embryos focused on amino acids by capillary electrophoresis with light-emitting diode-induced fluorescence detection
JF - Electrophoresis
VL - 39
IS - 23
SP - 3040-3048
EP - 3040-3048
PB - Verlag Chemie
SN - 01730835
KW - Amino acids
KW - Capillary electrophoresis with light emitting diode induced fluorescence detection;
UR - http://dx.doi.org/10.1002/elps.201800265
N2 - Assisted reproduction is a quickly developing field of reproductive medicine whose importance is growing every year due to the increasing number of patients suffering from infertility. As a result, there is a need for the continuous development and/or improvement of assisted reproductive technologies. This paper presents a new method for the in vitro measurement of the amino acid turnover of developing embryos based on capillary electrophoresis with light-emitting diode-induced fluorescence detection. Amino acids were derivatized with naphthalene-2,3-dicarboxaldehyde/NaCN, and the resulting fluorescent derivatives were baseline resolved within 25 min in a background electrolyte comprised of 50 mM sodium tetraborate, 73 mM sodium dodecyl sulphate, 5 mM sodium deoxycholate and 2.5 mM (2 hydroxypropyl)-beta cyclodextrin (pH 9.3). The migration time and the peak area repeatability (n = 10) were below 0.5 % and 4.3 %, respectively. The limits of detection ranged from 12.6 nM (histidine) to 39.3 nM (taurine). The developed method, which only requires 2 ul of raw sample, was successfully applied for determining the metabolic activity of human embryos exposed to different environmental stress conditions.
ER -
CELÁ, Andrea, Aleš MÁDR, Michal JEŠETA, Jana ŽÁKOVÁ, Igor CRHA a Zdeněk GLATZ. Study of metabolic activity of human embryos focused on amino acids by capillary electrophoresis with light-emitting diode-induced fluorescence detection. \textit{Electrophoresis}. Weinheim: Verlag Chemie, 2018, roč.~39, č.~23, s.~3040-3048. ISSN~0173-0835. Dostupné z: https://dx.doi.org/10.1002/elps.201800265.
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