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@proceedings{1430259,
author = {Růžičková, Vladislava and Botka, Tibor and Kavková, Michaela and Karpíšková, Renata and Koláčková, Ivana and Doškař, Jiří},
booktitle = {International Symposium on Staphylococci and Staphylococcal Infections | ISSSI 2018: 23 - 26 August 2018, Copenhagen, Denmark},
keywords = {Staphylococcus aureus; plasmid; livestock associated MRSA},
language = {eng},
title = {MOLECULAR ANALYSIS OF PLASMIDS ISOLATED FROM ST398 STRAINS OF STAPHYLOCOCCUS AUREUS},
url = {http://isssi2018.org},
year = {2018}
}
TY - CONF
ID - 1430259
AU - Růžičková, Vladislava - Botka, Tibor - Kavková, Michaela - Karpíšková, Renata - Koláčková, Ivana - Doškař, Jiří
PY - 2018
TI - MOLECULAR ANALYSIS OF PLASMIDS ISOLATED FROM ST398 STRAINS OF STAPHYLOCOCCUS AUREUS
KW - Staphylococcus aureus
KW - plasmid
KW - livestock associated MRSA
UR - http://isssi2018.org
N2 - Background: The strains of S. aureus ST398 are associated worldwide with livestock, humans and food products. They contain a broad spectrum of plasmid-borne genes conferring antibiotic resistance having impact in pathogenicity of the veterinary strains. Objectives: The strains isolated from raw cow’s milk samples were genotypically analyzed, and their plasmids were characterized in detail. Methods: The MLST, spa typing, prophage and virulence genes content were carried out. The whole plasmid sequences were determined in two selected strains. Results: All the host strains of ST398 were resistant to oxacillin and other 2 – 8 antibiotics. The spa type t011 predominated over type t034. All the strains, with excepting of one isolate, harbored the prophage of serogroup A. Plasmids isolated from eight strains were assigned to six REAP types. Two plasmids were sequenced. They showed a different size, REAP fingerprint and a sequence structure. Amplification of the aad gene encoding aminoglycoside 6-adenylyltransferase is conserved in predominated group of small plasmids. One plasmid carrying the resistance gene to lincosamides was unique in the studied strains. It harbored a great region of identical sequences with plasmid of Staphylococcus chromogenes, which indicates on the interspecies horizontal transfer of plasmid genes. Conclusions: This study reports a research data of not yet well described plasmids of clonal ST398 strains of S. aureus having an impact in veterinary medicine and food industry. This work was supported by the grant from Ministry of Agriculture of the Czech Republic (QJ1510216) and from the MUNI/A/0824/2017.
ER -
RŮŽIČKOVÁ, Vladislava, Tibor BOTKA, Michaela KAVKOVÁ, Renata KARPÍŠKOVÁ, Ivana KOLÁČKOVÁ a Jiří DOŠKAŘ. MOLECULAR ANALYSIS OF PLASMIDS ISOLATED FROM ST398 STRAINS OF STAPHYLOCOCCUS AUREUS. In \textit{International Symposium on Staphylococci and Staphylococcal Infections $|$ ISSSI 2018: 23 - 26 August 2018, Copenhagen, Denmark}. 2018.
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