MOLECULAR ANALYSIS OF PLASMIDS ISOLATED FROM ST398 STRAINS OF STAPHYLOCOCCUS AUREUS

RŮŽIČKOVÁ, Vladislava, Tibor BOTKA, Michaela KAVKOVÁ, Renata KARPÍŠKOVÁ, Ivana KOLÁČKOVÁ and Jiří DOŠKAŘ. MOLECULAR ANALYSIS OF PLASMIDS ISOLATED FROM ST398 STRAINS OF STAPHYLOCOCCUS AUREUS. In International Symposium on Staphylococci and Staphylococcal Infections | ISSSI 2018: 23 - 26 August 2018, Copenhagen, Denmark. 2018.

Basic information

• Original name: MOLECULAR ANALYSIS OF PLASMIDS ISOLATED FROM ST398 STRAINS OF STAPHYLOCOCCUS AUREUS
• Authors: RŮŽIČKOVÁ, Vladislava (203 Czech Republic, guarantor, belonging to the institution), Tibor BOTKA (203 Czech Republic, belonging to the institution), Michaela KAVKOVÁ (203 Czech Republic), Renata KARPÍŠKOVÁ (203 Czech Republic), Ivana KOLÁČKOVÁ (203 Czech Republic) and Jiří DOŠKAŘ (203 Czech Republic, belonging to the institution).
• Edition: International Symposium on Staphylococci and Staphylococcal Infections | ISSSI 2018: 23 - 26 August 2018, Copenhagen, Denmark, 2018.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 10606 Microbiology
• Country of publisher: Denmark
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• RIV identification code: RIV/00216224:14310/18:00100669
• Organization unit: Faculty of Science
• Keywords in English: Staphylococcus aureus; plasmid; livestock associated MRSA
• Tags: International impact, Reviewed

Abstract

Background: The strains of S. aureus ST398 are associated worldwide with livestock, humans and food products. They contain a broad spectrum of plasmid-borne genes conferring antibiotic resistance having impact in pathogenicity of the veterinary strains. Objectives: The strains isolated from raw cow’s milk samples were genotypically analyzed, and their plasmids were characterized in detail. Methods: The MLST, spa typing, prophage and virulence genes content were carried out. The whole plasmid sequences were determined in two selected strains. Results: All the host strains of ST398 were resistant to oxacillin and other 2 – 8 antibiotics. The spa type t011 predominated over type t034. All the strains, with excepting of one isolate, harbored the prophage of serogroup A. Plasmids isolated from eight strains were assigned to six REAP types. Two plasmids were sequenced. They showed a different size, REAP fingerprint and a sequence structure. Amplification of the aad gene encoding aminoglycoside 6-adenylyltransferase is conserved in predominated group of small plasmids. One plasmid carrying the resistance gene to lincosamides was unique in the studied strains. It harbored a great region of identical sequences with plasmid of Staphylococcus chromogenes, which indicates on the interspecies horizontal transfer of plasmid genes. Conclusions: This study reports a research data of not yet well described plasmids of clonal ST398 strains of S. aureus having an impact in veterinary medicine and food industry. This work was supported by the grant from Ministry of Agriculture of the Czech Republic (QJ1510216) and from the MUNI/A/0824/2017.

Links

• MUNI/A/0824/2017, interní kód MU: Name: Podpora výzkumné činnosti studentů molekulární biologie a genetiky 6 (Acronym: MolBiolGen)

Investor: Masaryk University, Category A

• QJ1510216, research and development project: Name: Fágová terapie infekcí vyvolaných Staphylococcus aureus v chovech hospodářských zvířat (Acronym: StafyFag)

Investor: Ministry of Agriculture of the CR