2018
Interactions of blood-feeding monogenean parasite with the fish host immune system
ILGOVÁ, Jana, Jiří SALÁT, Milan GELNAR a Martin KAŠNÝZákladní údaje
Originální název
Interactions of blood-feeding monogenean parasite with the fish host immune system
Autoři
Vydání
XIII. Slovenské a České parazitologické dny, 2018
Další údaje
Jazyk
angličtina
Typ výsledku
Konferenční abstrakt
Obor
10613 Zoology
Stát vydavatele
Slovensko
Utajení
není předmětem státního či obchodního tajemství
Organizační jednotka
Přírodovědecká fakulta
Klíčová slova anglicky
cystatin; Eudiplozoon nipponicum; immunomodulation; inflammation; cytokines
Změněno: 4. 9. 2018 09:57, Mgr. et Mgr. Jana Ilgová, Ph.D.
Anotace
V originále
Parasites use wide repertoire of molecules to manipulate the host’s immune system. Such interaction ensures the increased chances for parasite survival and reproduction. Nevertheless, the molecular communication between the fish host and parasitic worms from the group Monogenea have been topic omitted by the research. We chose experimental host-parasite system – blood-feeding, ectoparasitic Eudiplozoon nipponicum (Monogenea: Diplozoidae) and its typical host – common carp (Cyprinus carpio) and identified multiple parasite’s molecules with immunomodulatory potential. We selected cysteine peptidase inhibitor, also called type I cystatin or stefin (EnStef), which we recognized as relatively highly expressed, for further immune assays. After in vitro incubation of fish macrophages with recombinant EnStef we analysed the expression levels of pro- and anti-inflammatory cytokines (TNF-alpha, IL1-beta, IL-6, IL-8, IL-10) produced by the cells. Our preliminary results suggest that EnStef may cause down-regulation of studied cytokines.
Návaznosti
GBP505/12/G112, projekt VaV |
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