Immunosensors and immunoassays for detection of clinically
important analytes

p 2018

Immunosensors and immunoassays for detection of clinically important analytes

FARKA, Zdeněk

Základní údaje

Originální název

Immunosensors and immunoassays for detection of clinically important analytes

Autoři

FARKA, Zdeněk

Vydání

Invited lecture at Institute of Macromolecular Chemistry AS CR, 2018

Další údaje

Jazyk

angličtina

Typ výsledku

Vyžádané přednášky

Obor

10406 Analytical chemistry

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Organizační jednotka

Středoevropský technologický institut

Klíčová slova anglicky

Biosensor; Immunosensor; Immunoassay; Nanoparticle; Single-molecule detection

Změněno: 8. 9. 2018 10:35, doc. Mgr. Zdeněk Farka, Ph.D.

Anotace

V originále

Highly sensitive and specific bioanalysis is essential in all fields of human activities from food industry to clinical diagnostics. Among many analytical techniques, immunoassays and immunosensors are gaining attention due to the high specificity provided by antibodies and excellent sensitivity provided by various labels. We have employed immunosensors for the detection of Salmonella Typhimurium, which belongs to leading agents of gastrointestinal diseases. The point-of-care sensor was based on electrochemical impedance spectroscopy and screen-printed electrodes modified by specific antibody. We have further developed a signal enhancement by enzymatic precipitation for highly sensitive laboratory-based detection of Salmonella using surface plasmon resonance. Photon-upconversion nanoparticles (UCNPs) are lanthanide-doped nanocrystals, which convert near-infrared light to light of shorter wavelengths. We have exploited UCNPs as a label in upconversion-linked immunoassay for the detection of pharmaceutical diclofenac, which is a common micropollutant in waters. The low background and high photostability make UCNPs a powerful tool for detection of single molecules. We have developed an optical approach for visualizing individual UCNPs and applied it for the sensitive detection of the cancer biomarker prostate specific antigen. The noise-surpassing digital readout provided single particle resolution with almost no instrumental background.

Návaznosti

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

FARKA, Zdeněk. Immunosensors and immunoassays for detection of clinically important analytes. In Invited lecture at Institute of Macromolecular Chemistry AS CR. 2018.

@misc{1433476,
   author = {Farka, Zdeněk},
   booktitle = {Invited lecture at Institute of Macromolecular Chemistry AS CR},
   keywords = {Biosensor; Immunosensor; Immunoassay; Nanoparticle; Single-molecule detection},
   language = {eng},
   title = {Immunosensors and immunoassays for detection of clinically important analytes},
   year = {2018}
}

TY  - SLIDE
ID  - 1433476
AU  - Farka, Zdeněk
PY  - 2018
TI  - Immunosensors and immunoassays for detection of clinically important analytes
KW  - Biosensor
KW  - Immunosensor
KW  - Immunoassay
KW  - Nanoparticle
KW  - Single-molecule detection
N2  - Highly sensitive and specific bioanalysis is essential in all fields of human activities from food industry to clinical diagnostics. Among many analytical techniques, immunoassays and immunosensors are gaining attention due to the high specificity provided by antibodies and excellent sensitivity provided by various labels. We have employed immunosensors for the detection of Salmonella Typhimurium, which belongs to leading agents of gastrointestinal diseases. The point-of-care sensor was based on electrochemical impedance spectroscopy and screen-printed electrodes modified by specific antibody. We have further developed a signal enhancement by enzymatic precipitation for highly sensitive laboratory-based detection of Salmonella using surface plasmon resonance. Photon-upconversion nanoparticles (UCNPs) are lanthanide-doped nanocrystals, which convert near-infrared light to light of shorter wavelengths. We have exploited UCNPs as a label in upconversion-linked immunoassay for the detection of pharmaceutical diclofenac, which is a common micropollutant in waters. The low background and high photostability make UCNPs a powerful tool for detection of single molecules. We have developed an optical approach for visualizing individual UCNPs and applied it for the sensitive detection of the cancer biomarker prostate specific antigen. The noise-surpassing digital readout provided single particle resolution with almost no instrumental background.
ER  -

FARKA, Zdeněk. Immunosensors and immunoassays for detection of clinically important analytes. In \textit{Invited lecture at Institute of Macromolecular Chemistry AS CR}. 2018.

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