Osteoarthritic process modifies expression response to NiTi
alloy presence

J 2018

Osteoarthritic process modifies expression response to NiTi alloy presence

VÁLKOVÁ, Lucie, Jana VEVERKOVÁ, Monika PÁVKOVÁ GOLDBERGOVÁ, Adam WEISER, Antonín DLOUHÝ et. al.

Základní údaje

Originální název

Osteoarthritic process modifies expression response to NiTi alloy presence

Autoři

VÁLKOVÁ, Lucie (203 Česká republika, domácí), Jana VEVERKOVÁ (203 Česká republika, domácí), Monika PÁVKOVÁ GOLDBERGOVÁ (203 Česká republika, garant, domácí), Adam WEISER (203 Česká republika) a Antonín DLOUHÝ

Vydání

Journal of Materials Science: Materials in Medicine, Dordrecht, Springer, 2018, 0957-4530

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

20601 Medical engineering

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.467

Kód RIV

RIV/00216224:14110/18:00101160

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1007/s10856-018-6156-z

UT WoS

000443368900001

Klíčová slova anglicky

Osteoarthritic process; Nickel-titanium alloy

Štítky

14110518, EL OK, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 10. 2. 2019 17:09, Soňa Böhmová

Anotace

V originále

Nickel-titanium alloy (nitinol, NiTi) is a biomaterial with unique thermal shape memory, superelasticity and high damping properties. Therefore NiTi has been used in medical applications. In this in vitro study, the effect of NiTi alloy (with two surface modifications - helium and hydrogen) on gene expression profile of selected interleukins (IL-1, IL-6 and IL-8) and matrix metalloproteinases (MMP-1 and MMP-2) in human physiological osteoblasts and human osteoarthritic osteoblasts was examined to respond to a question of the different behavior of bone tissue in the implantation of metallic materials in the presence of cells affected by the osteoarthritic process. The cells were cultivated in contact with NiTi and with or without LPS (bacterial lipolysaccharide). Changes in expression of target genes were calculated by 2(-Ct) method. An increased gene expression of IL-1 in osteoarthritic osteoblasts, with even higher expression in cells collected directly from the metal surface was observed. In case of physiological osteoblasts, the change in expression was detected after LPS treatment in cells surrounding the disc. Higher expression levels of IL-8 were observed in osteoarthritic osteoblasts after NiTi treatment in contact with alloy, and in physiological osteoblasts without relation to location in combination of NiTi and LPS. IL-6 was slightly increased in physiological osteoblastes after application of LPS. MMP-1 expression level was obviously significantly higher in osteoarthritic osteoblasts with differences regarding the metal surface and location. MMP-2 expression was decreased in both cell lines after LPS treatment. In conclusion, results of present study show that the NiTi alloy and the treatment by LPS, especially repeated doses of LPS, change the gene expression of selected ILs and MMPs in human osteoblast cell cultures. Some of the changes were depicted solely to osteoarthritic osteoblasts.

Návaznosti

GA15-16336S, projekt VaV

Název: Intersticiální příměsi v tvarově-paměťových slitinách na bázi NiTi

Investor: Grantová agentura ČR, Intersticiální příměsi v tvarově-paměťových slitinách na bázi NiTi

Citovat

VÁLKOVÁ, Lucie, Jana VEVERKOVÁ, Monika PÁVKOVÁ GOLDBERGOVÁ, Adam WEISER a Antonín DLOUHÝ. Osteoarthritic process modifies expression response to NiTi alloy presence. Journal of Materials Science: Materials in Medicine. Dordrecht: Springer, 2018, roč. 29, č. 9, s. 1-12. ISSN 0957-4530. Dostupné z: https://dx.doi.org/10.1007/s10856-018-6156-z.

@article{1435016,
   author = {Válková, Lucie and Veverková, Jana and Pávková Goldbergová, Monika and Weiser, Adam and Dlouhý, Antonín},
   article_location = {Dordrecht},
   article_number = {9},
   doi = {http://dx.doi.org/10.1007/s10856-018-6156-z},
   keywords = {Osteoarthritic process; Nickel-titanium alloy},
   language = {eng},
   issn = {0957-4530},
   journal = {Journal of Materials Science: Materials in Medicine},
   title = {Osteoarthritic process modifies expression response to NiTi alloy presence},
   volume = {29},
   year = {2018}
}

TY  - JOUR
ID  - 1435016
AU  - Válková, Lucie - Veverková, Jana - Pávková Goldbergová, Monika - Weiser, Adam - Dlouhý, Antonín
PY  - 2018
TI  - Osteoarthritic process modifies expression response to NiTi alloy presence
JF  - Journal of Materials Science: Materials in Medicine
VL  - 29
IS  - 9
SP  - 1-12
EP  - 1-12
PB  - Springer
SN  - 09574530
KW  - Osteoarthritic process
KW  - Nickel-titanium alloy
N2  - Nickel-titanium alloy (nitinol, NiTi) is a biomaterial with unique thermal shape memory, superelasticity and high damping properties. Therefore NiTi has been used in medical applications. In this in vitro study, the effect of NiTi alloy (with two surface modifications - helium and hydrogen) on gene expression profile of selected interleukins (IL-1, IL-6 and IL-8) and matrix metalloproteinases (MMP-1 and MMP-2) in human physiological osteoblasts and human osteoarthritic osteoblasts was examined to respond to a question of the different behavior of bone tissue in the implantation of metallic materials in the presence of cells affected by the osteoarthritic process. The cells were cultivated in contact with NiTi and with or without LPS (bacterial lipolysaccharide). Changes in expression of target genes were calculated by 2(-Ct) method. An increased gene expression of IL-1 in osteoarthritic osteoblasts, with even higher expression in cells collected directly from the metal surface was observed. In case of physiological osteoblasts, the change in expression was detected after LPS treatment in cells surrounding the disc. Higher expression levels of IL-8 were observed in osteoarthritic osteoblasts after NiTi treatment in contact with alloy, and in physiological osteoblasts without relation to location in combination of NiTi and LPS. IL-6 was slightly increased in physiological osteoblastes after application of LPS. MMP-1 expression level was obviously significantly higher in osteoarthritic osteoblasts with differences regarding the metal surface and location. MMP-2 expression was decreased in both cell lines after LPS treatment. In conclusion, results of present study show that the NiTi alloy and the treatment by LPS, especially repeated doses of LPS, change the gene expression of selected ILs and MMPs in human osteoblast cell cultures. Some of the changes were depicted solely to osteoarthritic osteoblasts.
ER  -

VÁLKOVÁ, Lucie, Jana VEVERKOVÁ, Monika PÁVKOVÁ GOLDBERGOVÁ, Adam WEISER a Antonín DLOUHÝ. Osteoarthritic process modifies expression response to NiTi alloy presence. \textit{Journal of Materials Science: Materials in Medicine}. Dordrecht: Springer, 2018, roč.~29, č.~9, s.~1-12. ISSN~0957-4530. Dostupné z: https://dx.doi.org/10.1007/s10856-018-6156-z.

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