TLR9 expression in the Choroid Plexus after subarachnoid
hemorrhage

a 2018

TLR9 expression in the Choroid Plexus after subarachnoid hemorrhage

LEVIN, Shahaf, Peter SOLÁR, Ilona KLUSÁKOVÁ, Petr DUBOVÝ, Marek JOUKAL et. al.

Základní údaje

Originální název

TLR9 expression in the Choroid Plexus after subarachnoid hemorrhage

Autoři

LEVIN, Shahaf (376 Izrael), Peter SOLÁR (703 Slovensko), Ilona KLUSÁKOVÁ (203 Česká republika), Petr DUBOVÝ (203 Česká republika) a Marek JOUKAL (203 Česká republika, garant, domácí)

Vydání

Morphology 2018, 2018

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

30103 Neurosciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14110/18:00103665

Organizační jednotka

Lékařská fakulta

ISBN

978-80-88064-35-0

Klíčová slova anglicky

Subarachnoid hemorrhage; TLR9; Choroid Plexus

Změněno: 5. 12. 2018 09:03, doc. MUDr. Marek Joukal, Ph.D.

Anotace

V originále

Subarachnoid hemorrhage (SAH) is a life-threatening emergency. The main noticeable changes after the onset of bleeding are reactive inflammatory reaction of neurons, glial cells and development of hydrocephalus. These changes are attributed to the effects of blood disintegration products, especially metabolites of heme. TLR9 may affect the tight junctions among the choroid plexus (CP) ependymal cells resulting in increased cerebrospinal fluid (CSF) production and increased intracranial pressure. Therefore, the aim of our study was to assess the changes of TLR9 in the CP after SAH. Wistar rats (n=40, males) were used in our experiments. Injection of autologous arterial blood into the cisterna magna was performed on experimental rats, while control animals received artificial CSF. The animals were left to survive for 1, 3 and 7 days from the application. Experimental and control rats were sacrificed together with naive rats and perfused transcardially with Zamboni´s fixative. Coronal cryostat sections through the brains were cut and immunostained for TLR9. The intensity of TLR9 immunofluorescence in the CP was quantified and statistically analyzed. The presence of TLR9 immunofluorescence was found in the cytoplasm of CP cuboidal cells in all animal groups. Statistically significant increase in TLR9 immunofluorescence was found in the CP of SAH animals in comparison to control and naive animals. This intensity increased with time of survival for 3 days. Our results clearly indicate that the CP reacts to SAH with an increase in TLR9 expression. These changes might be associated with increased intracranial pressure after SAH.

Návaznosti

MUNI/A/0908/2017, interní kód MU

Název: Reakce plexus choroideus laboratorního potkana na subarachnoidální krvácení

Investor: Masarykova univerzita, Reakce plexus choroideus laboratorního potkana na subarachnoidální krvácení, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Citovat

LEVIN, Shahaf, Peter SOLÁR, Ilona KLUSÁKOVÁ, Petr DUBOVÝ a Marek JOUKAL. TLR9 expression in the Choroid Plexus after subarachnoid hemorrhage. In Morphology 2018. 2018. ISBN 978-80-88064-35-0.

@proceedings{1435522,
   author = {Levin, Shahaf and Solár, Peter and Klusáková, Ilona and Dubový, Petr and Joukal, Marek},
   booktitle = {Morphology 2018},
   keywords = {Subarachnoid hemorrhage; TLR9; Choroid Plexus},
   language = {eng},
   isbn = {978-80-88064-35-0},
   title = {TLR9 expression in the Choroid Plexus after subarachnoid hemorrhage},
   year = {2018}
}

TY  - CONF
ID  - 1435522
AU  - Levin, Shahaf - Solár, Peter - Klusáková, Ilona - Dubový, Petr - Joukal, Marek
PY  - 2018
TI  - TLR9 expression in the Choroid Plexus after subarachnoid hemorrhage
SN  - 9788088064350
KW  - Subarachnoid hemorrhage
KW  - TLR9
KW  - Choroid Plexus
N2  - Subarachnoid hemorrhage (SAH) is a life-threatening emergency. The main noticeable changes after the onset of bleeding are reactive inflammatory reaction of neurons, glial cells and development of hydrocephalus. These changes are attributed to the effects of blood disintegration products, especially metabolites of heme. TLR9 may affect the tight junctions among the choroid plexus (CP) ependymal cells resulting in increased cerebrospinal fluid (CSF) production and increased intracranial pressure. Therefore, the aim of our study was to assess the changes of TLR9 in the CP after SAH. Wistar rats (n=40, males) were used in our experiments. Injection of autologous arterial blood into the cisterna magna was performed on experimental rats, while control animals received artificial CSF. The animals were left to survive for 1, 3 and 7 days from the application. Experimental and control rats were sacrificed together with naive rats and perfused transcardially with Zamboni´s fixative. Coronal cryostat sections through the brains were cut and immunostained for TLR9. The intensity of TLR9 immunofluorescence in the CP was quantified and statistically analyzed. The presence of TLR9 immunofluorescence was found in the cytoplasm of CP cuboidal cells in all animal groups. Statistically significant increase in TLR9 immunofluorescence was found in the CP of SAH animals in comparison to control and naive animals. This intensity increased with time of survival for 3 days. Our results clearly indicate that the CP reacts to SAH with an increase in TLR9 expression. These changes might be associated with increased intracranial pressure after SAH.
ER  -

LEVIN, Shahaf, Peter SOLÁR, Ilona KLUSÁKOVÁ, Petr DUBOVÝ a Marek JOUKAL. TLR9 expression in the Choroid Plexus after subarachnoid hemorrhage. In \textit{Morphology 2018}. 2018. ISBN~978-80-88064-35-0.

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