| MAKHNEVA, Ekaterina, Zdeněk FARKA, Petr SKLÁDAL a Lenka ZAJÍČKOVÁ. Cyclopropylamine plasma polymer surfaces for label-free SPR and QCM immunosensing of Salmonella. Sensors and Actuators B: Chemical. Amsterdam: Elsevier B.V., 2018, roč. 276, DEC, s. 447-455. ISSN 0925-4005. Dostupné z: https://dx.doi.org/10.1016/j.snb.2018.08.055. |
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@article{1436440,
author = {Makhneva, Ekaterina and Farka, Zdeněk and Skládal, Petr and Zajíčková, Lenka},
article_location = {Amsterdam},
article_number = {DEC},
doi = {http://dx.doi.org/10.1016/j.snb.2018.08.055},
keywords = {Plasma polymerization; Amine films; Surface plasmon resonance; Quartz crystal microbalance; Immunosensor; Label-free detection},
language = {eng},
issn = {0925-4005},
journal = {Sensors and Actuators B: Chemical},
title = {Cyclopropylamine plasma polymer surfaces for label-free SPR and QCM immunosensing of Salmonella},
url = {https://www.sciencedirect.com/science/article/pii/S0925400518314680},
volume = {276},
year = {2018}
}
TY - JOUR
ID - 1436440
AU - Makhneva, Ekaterina - Farka, Zdeněk - Skládal, Petr - Zajíčková, Lenka
PY - 2018
TI - Cyclopropylamine plasma polymer surfaces for label-free SPR and QCM immunosensing of Salmonella
JF - Sensors and Actuators B: Chemical
VL - 276
IS - DEC
SP - 447-455
EP - 447-455
PB - Elsevier B.V.
SN - 09254005
KW - Plasma polymerization
KW - Amine films
KW - Surface plasmon resonance
KW - Quartz crystal microbalance
KW - Immunosensor
KW - Label-free detection
UR - https://www.sciencedirect.com/science/article/pii/S0925400518314680
N2 - We report on the first successful application of nitrogen-containing functional surfaces prepared by plasma polymerization in surface plasmon resonance (SPR) immunosensing. The plasma polymers (PPs) were deposited from cyclopropylamine (CPA) vapors onto gold surfaces of SPR and quartz crystal microbalance (QCM) sensors. It provided an excellent platform for a stable immobilization of antibodies using glutaraldehyde (GA) activation. The performance of the SPR immunosensor was proven by a model pair of monoclonal antibody (Ab) AL-01 and human serum albumin as well as by the selective detection of bacterial pathogen Salmonella Typhimurium using the immobilized anti-Salmonella Ab. A baseline drift of the measured SPR signal, caused by the long-term reactivity of CPA plasma polymers, was well stabilized by 18 h immersion in phosphate buffered saline prior to the GA activation. The immunosensors can be successfully regenerated several times using 10 mM NaOH. The limit of detection of 10^5 CFU/mL and a wide linear response were achieved for Salmonella. The assay parameters are comparable to the conventional label-free methods. However, the replacement of conventional immobilization matrix with the CPA PPs offers an advantage of excellent adhesion to gold QCM and SPR surfaces achieved by fast and eco-friendly procedure compatible with the vacuum deposition of gold.
ER -
MAKHNEVA, Ekaterina, Zdeněk FARKA, Petr SKLÁDAL a Lenka ZAJÍČKOVÁ. Cyclopropylamine plasma polymer surfaces for label-free SPR and QCM immunosensing of Salmonella. \textit{Sensors and Actuators B: Chemical}. Amsterdam: Elsevier B.V., 2018, roč.~276, DEC, s.~447-455. ISSN~0925-4005. Dostupné z: https://dx.doi.org/10.1016/j.snb.2018.08.055.
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