Deleterious Effect of Advanced CKD on Glyoxalase System
Activity not Limited to Diabetes Aetiology

J 2018

Deleterious Effect of Advanced CKD on Glyoxalase System Activity not Limited to Diabetes Aetiology

PÁCAL, Lukáš, Katarína CHALÁSOVÁ, Anna PLESKAČOVÁ, Jitka REHOROVA, Josef TOMANDL et. al.

Základní údaje

Originální název

Deleterious Effect of Advanced CKD on Glyoxalase System Activity not Limited to Diabetes Aetiology

Autoři

PÁCAL, Lukáš (203 Česká republika, garant, domácí), Katarína CHALÁSOVÁ (703 Slovensko, domácí), Anna PLESKAČOVÁ (203 Česká republika, domácí), Jitka REHOROVA (203 Česká republika), Josef TOMANDL (203 Česká republika) a Kateřina KAŇKOVÁ (203 Česká republika, domácí)

Vydání

International Journal of Molecular Sciences, Basel, Switzerland, MDPI AG, 2018, 1422-0067

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 4.183

Kód RIV

RIV/00216224:14110/18:00101254

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.3390/ijms19051517

UT WoS

000435297000253

Klíčová slova anglicky

glyoxalase; diabetes; chronic kidney disease; diabetic nephropathy

Štítky

14110512, 14110518, EL OK, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 9. 2. 2019 22:00, Soňa Böhmová

Anotace

V originále

Methylglyoxal production is increased in diabetes. Methylglyoxal is efficiently detoxified by enzyme glyoxalase 1 (GLO1). The aim was to study the effect of diabetic and CKD milieu on (a) GLO1 gene expression in peripheral blood mononuclear cells; (b) GLO1 protein levels in whole blood; and (c) GLO1 activity in RBCs in vivo in diabetic vs. non-diabetic subjects with normal or slightly reduced vs. considerably reduced renal function (CKD1-2 vs. CKD3-4). A total of 83 subjects were included in the study. Gene expression was measured using real-time PCR, and protein levels were quantified using Western blotting. Erythrocyte GLO1 activity was measured spectrophotometrically. GLO1 gene expression was significantly higher in subjects with CKD1-2 compared to CKD3-4. GLO1 protein level was lower in diabetics than in non-diabetics. GLO1 activity in RBCs differed between the four groups being significantly higher in diabetics with CKD1-2 vs. healthy subjects and vs. nondiabeticsfig with CKD3-4. GLO1 activity was significantly higher in diabetics compared to nondiabetics. In conclusion, both diabetes and CKD affects the glyoxalase system. It appears that CKD in advanced stages has prevailing and suppressive effects compared to hyperglycaemia. CKD decreases GLO1 gene expression and protein levels (together with diabetes) without concomitant changes of GLO1 activity.

Návaznosti

GA16-14829S, projekt VaV

Název: Efekt diabetického mikroprostředí na vybrané procesy při vzniku kolorektálního karcinomu, jeho klinický průběh a odpověď na terapii

Investor: Grantová agentura ČR, Efekt diabetického mikroprostředí na vybrané procesy při vzniku kolorektálního karcinomu, jeho klinický průběh a odpověď na terapii

Citovat

PÁCAL, Lukáš, Katarína CHALÁSOVÁ, Anna PLESKAČOVÁ, Jitka REHOROVA, Josef TOMANDL a Kateřina KAŇKOVÁ. Deleterious Effect of Advanced CKD on Glyoxalase System Activity not Limited to Diabetes Aetiology. International Journal of Molecular Sciences. Basel, Switzerland: MDPI AG, 2018, roč. 19, č. 5, s. 1-9. ISSN 1422-0067. Dostupné z: https://dx.doi.org/10.3390/ijms19051517.

@article{1448576,
   author = {Pácal, Lukáš and Chalásová, Katarína and Pleskačová, Anna and Rehorova, Jitka and Tomandl, Josef and Kaňková, Kateřina},
   article_location = {Basel, Switzerland},
   article_number = {5},
   doi = {http://dx.doi.org/10.3390/ijms19051517},
   keywords = {glyoxalase; diabetes; chronic kidney disease; diabetic nephropathy},
   language = {eng},
   issn = {1422-0067},
   journal = {International Journal of Molecular Sciences},
   title = {Deleterious Effect of Advanced CKD on Glyoxalase System Activity not Limited to Diabetes Aetiology},
   volume = {19},
   year = {2018}
}

TY  - JOUR
ID  - 1448576
AU  - Pácal, Lukáš - Chalásová, Katarína - Pleskačová, Anna - Rehorova, Jitka - Tomandl, Josef - Kaňková, Kateřina
PY  - 2018
TI  - Deleterious Effect of Advanced CKD on Glyoxalase System Activity not Limited to Diabetes Aetiology
JF  - International Journal of Molecular Sciences
VL  - 19
IS  - 5
SP  - 1-9
EP  - 1-9
PB  - MDPI AG
SN  - 14220067
KW  - glyoxalase
KW  - diabetes
KW  - chronic kidney disease
KW  - diabetic nephropathy
N2  - Methylglyoxal production is increased in diabetes. Methylglyoxal is efficiently detoxified by enzyme glyoxalase 1 (GLO1). The aim was to study the effect of diabetic and CKD milieu on (a) GLO1 gene expression in peripheral blood mononuclear cells; (b) GLO1 protein levels in whole blood; and (c) GLO1 activity in RBCs in vivo in diabetic vs. non-diabetic subjects with normal or slightly reduced vs. considerably reduced renal function (CKD1-2 vs. CKD3-4). A total of 83 subjects were included in the study. Gene expression was measured using real-time PCR, and protein levels were quantified using Western blotting. Erythrocyte GLO1 activity was measured spectrophotometrically. GLO1 gene expression was significantly higher in subjects with CKD1-2 compared to CKD3-4. GLO1 protein level was lower in diabetics than in non-diabetics. GLO1 activity in RBCs differed between the four groups being significantly higher in diabetics with CKD1-2 vs. healthy subjects and vs. nondiabeticsfig with CKD3-4. GLO1 activity was significantly higher in diabetics compared to nondiabetics. In conclusion, both diabetes and CKD affects the glyoxalase system. It appears that CKD in advanced stages has prevailing and suppressive effects compared to hyperglycaemia. CKD decreases GLO1 gene expression and protein levels (together with diabetes) without concomitant changes of GLO1 activity.
ER  -

PÁCAL, Lukáš, Katarína CHALÁSOVÁ, Anna PLESKAČOVÁ, Jitka REHOROVA, Josef TOMANDL a Kateřina KAŇKOVÁ. Deleterious Effect of Advanced CKD on Glyoxalase System Activity not Limited to Diabetes Aetiology. \textit{International Journal of Molecular Sciences}. Basel, Switzerland: MDPI AG, 2018, roč.~19, č.~5, s.~1-9. ISSN~1422-0067. Dostupné z: https://dx.doi.org/10.3390/ijms19051517.

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