2018
Deleterious Effect of Advanced CKD on Glyoxalase System Activity not Limited to Diabetes Aetiology
PÁCAL, Lukáš, Katarína CHALÁSOVÁ, Anna PLESKAČOVÁ, Jitka REHOROVA, Josef TOMANDL et. al.Základní údaje
Originální název
Deleterious Effect of Advanced CKD on Glyoxalase System Activity not Limited to Diabetes Aetiology
Autoři
PÁCAL, Lukáš (203 Česká republika, garant, domácí), Katarína CHALÁSOVÁ (703 Slovensko, domácí), Anna PLESKAČOVÁ (203 Česká republika, domácí), Jitka REHOROVA (203 Česká republika), Josef TOMANDL (203 Česká republika) a Kateřina KAŇKOVÁ (203 Česká republika, domácí)
Vydání
International Journal of Molecular Sciences, Basel, Switzerland, MDPI AG, 2018, 1422-0067
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
10608 Biochemistry and molecular biology
Stát vydavatele
Švýcarsko
Utajení
není předmětem státního či obchodního tajemství
Impakt faktor
Impact factor: 4.183
Kód RIV
RIV/00216224:14110/18:00101254
Organizační jednotka
Lékařská fakulta
UT WoS
000435297000253
Klíčová slova anglicky
glyoxalase; diabetes; chronic kidney disease; diabetic nephropathy
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 9. 2. 2019 22:00, Soňa Böhmová
Anotace
V originále
Methylglyoxal production is increased in diabetes. Methylglyoxal is efficiently detoxified by enzyme glyoxalase 1 (GLO1). The aim was to study the effect of diabetic and CKD milieu on (a) GLO1 gene expression in peripheral blood mononuclear cells; (b) GLO1 protein levels in whole blood; and (c) GLO1 activity in RBCs in vivo in diabetic vs. non-diabetic subjects with normal or slightly reduced vs. considerably reduced renal function (CKD1-2 vs. CKD3-4). A total of 83 subjects were included in the study. Gene expression was measured using real-time PCR, and protein levels were quantified using Western blotting. Erythrocyte GLO1 activity was measured spectrophotometrically. GLO1 gene expression was significantly higher in subjects with CKD1-2 compared to CKD3-4. GLO1 protein level was lower in diabetics than in non-diabetics. GLO1 activity in RBCs differed between the four groups being significantly higher in diabetics with CKD1-2 vs. healthy subjects and vs. nondiabeticsfig with CKD3-4. GLO1 activity was significantly higher in diabetics compared to nondiabetics. In conclusion, both diabetes and CKD affects the glyoxalase system. It appears that CKD in advanced stages has prevailing and suppressive effects compared to hyperglycaemia. CKD decreases GLO1 gene expression and protein levels (together with diabetes) without concomitant changes of GLO1 activity.
Návaznosti
GA16-14829S, projekt VaV |
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