Clinicopathological correlation of tumor-associated macrophages
in Ewing sarcoma

J 2018

Clinicopathological correlation of tumor-associated macrophages in Ewing sarcoma

HANDL, Marek, Markéta HERMANOVÁ, Sylva HOTÁRKOVÁ, Jiří JARKOVSKÝ, Peter MÚDRY et. al.

Základní údaje

Originální název

Clinicopathological correlation of tumor-associated macrophages in Ewing sarcoma

Autoři

HANDL, Marek (203 Česká republika), Markéta HERMANOVÁ (203 Česká republika, domácí), Sylva HOTÁRKOVÁ (203 Česká republika, domácí), Jiří JARKOVSKÝ (203 Česká republika, domácí), Peter MÚDRY (203 Česká republika, domácí), Tetiana SHATOKHINA (804 Ukrajina, domácí), Marcela VESELA (203 Česká republika), Jaroslav ŠTĚRBA (203 Česká republika, domácí) a Iva ZAMBO (203 Česká republika, garant, domácí)

Vydání

Biomedical Papers, Olomouc: Palacky University, Olomouc, Palacky University, 2018, 1213-8118

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30204 Oncology

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.141

Kód RIV

RIV/00216224:14110/18:00104043

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.5507/bp.2017.049

UT WoS

000428951000010

Klíčová slova anglicky

tumor associated macrophages; Ewing sarcoma; immunohistochemistry; CD68; CD163; prognosis

Štítky

14110112, 14110321, 14119612, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 9. 2. 2019 21:40, Soňa Böhmová

Anotace

V originále

Aims. Tumor-associated macrophages (TAMs) are known markers playing complex roles in tumorigenesis. However, the function of TAMs in a variety of malignancies is not yet fully understood. The aim of this pilot study was to quantify the density of TAMs in Ewing sarcoma and to determine the correlation between TAMs and clinicopathological parameters. Methods. Using immunohistochemistry, the expressions of CD68 and CD163 were examined in 24 tissue samples of Ewing sarcoma of bone. The density of CD68 and CD163-positive TAMs was analyzed quantitatively and semi-quantitatively and statistically correlated with clinical parameters. Results. CD163-positive TAMs outnumbered CD68-positive cells (median of 130 vs 96, respectively). No statistically significant relatio nship was found between density of CD68-positive cells, clinical parameters or prognosis. However, high levels of CD163-positive TAMs were associated with localized disease (P=0.008). In cases with a higher density of CD163-positive cells, a trend toward longer survival was revealed (P=0.063). Conclusion. This is the first study that has quantified CD163 expression in TAMs in Ewing sarcoma and showed its possible prognostic value. CD163 was confirmed to be a more specific marker of macrophages than CD68. CD163 is not an exclusive hallmark of M2 macrophages.

Návaznosti

MUNI/FR/0377/2014, interní kód MU

Název: Informační prostředí pro multidisciplinární vědeckou edukaci doktorandů a studentů v programu P-PooL LF MU

Investor: Masarykova univerzita, Informační prostředí pro multidisciplinární vědeckou edukaci doktorandů a studentů v programu P-PooL LF MU

Citovat

HANDL, Marek, Markéta HERMANOVÁ, Sylva HOTÁRKOVÁ, Jiří JARKOVSKÝ, Peter MÚDRY, Tetiana SHATOKHINA, Marcela VESELA, Jaroslav ŠTĚRBA a Iva ZAMBO. Clinicopathological correlation of tumor-associated macrophages in Ewing sarcoma. Biomedical Papers, Olomouc: Palacky University. Olomouc: Palacky University, 2018, roč. 162, č. 1, s. 54-60. ISSN 1213-8118. Dostupné z: https://dx.doi.org/10.5507/bp.2017.049.

@article{1449676,
   author = {Handl, Marek and Hermanová, Markéta and Hotárková, Sylva and Jarkovský, Jiří and Múdry, Peter and Shatokhina, Tetiana and Vesela, Marcela and Štěrba, Jaroslav and Zambo, Iva},
   article_location = {Olomouc},
   article_number = {1},
   doi = {http://dx.doi.org/10.5507/bp.2017.049},
   keywords = {tumor associated macrophages; Ewing sarcoma; immunohistochemistry; CD68; CD163; prognosis},
   language = {eng},
   issn = {1213-8118},
   journal = {Biomedical Papers, Olomouc: Palacky University},
   title = {Clinicopathological correlation of tumor-associated macrophages in Ewing sarcoma},
   volume = {162},
   year = {2018}
}

TY  - JOUR
ID  - 1449676
AU  - Handl, Marek - Hermanová, Markéta - Hotárková, Sylva - Jarkovský, Jiří - Múdry, Peter - Shatokhina, Tetiana - Vesela, Marcela - Štěrba, Jaroslav - Zambo, Iva
PY  - 2018
TI  - Clinicopathological correlation of tumor-associated macrophages in Ewing sarcoma
JF  - Biomedical Papers, Olomouc: Palacky University
VL  - 162
IS  - 1
SP  - 54-60
EP  - 54-60
PB  - Palacky University
SN  - 12138118
KW  - tumor associated macrophages
KW  - Ewing sarcoma
KW  - immunohistochemistry
KW  - CD68
KW  - CD163
KW  - prognosis
N2  - Aims. Tumor-associated macrophages (TAMs) are known markers playing complex roles in tumorigenesis. However, the function of TAMs in a variety of malignancies is not yet fully understood. The aim of this pilot study was to quantify the density of TAMs in Ewing sarcoma and to determine the correlation between TAMs and clinicopathological parameters. Methods. Using immunohistochemistry, the expressions of CD68 and CD163 were examined in 24 tissue samples of Ewing sarcoma of bone. The density of CD68 and CD163-positive TAMs was analyzed quantitatively and semi-quantitatively and statistically correlated with clinical parameters. Results. CD163-positive TAMs outnumbered CD68-positive cells (median of 130 vs 96, respectively). No statistically significant relatio nship was found between density of CD68-positive cells, clinical parameters or prognosis. However, high levels of CD163-positive TAMs were associated with localized disease (P=0.008). In cases with a higher density of CD163-positive cells, a trend toward longer survival was revealed (P=0.063). Conclusion. This is the first study that has quantified CD163 expression in TAMs in Ewing sarcoma and showed its possible prognostic value. CD163 was confirmed to be a more specific marker of macrophages than CD68. CD163 is not an exclusive hallmark of M2 macrophages.
ER  -

HANDL, Marek, Markéta HERMANOVÁ, Sylva HOTÁRKOVÁ, Jiří JARKOVSKÝ, Peter MÚDRY, Tetiana SHATOKHINA, Marcela VESELA, Jaroslav ŠTĚRBA a Iva ZAMBO. Clinicopathological correlation of tumor-associated macrophages in Ewing sarcoma. \textit{Biomedical Papers, Olomouc: Palacky University}. Olomouc: Palacky University, 2018, roč.~162, č.~1, s.~54-60. ISSN~1213-8118. Dostupné z: https://dx.doi.org/10.5507/bp.2017.049.

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