J 2018

Effect of prostate cancer cell line supernatant on functional polarization in macrophages

MAZALOVA, L., Z. SLADEK, Martina RAUDENSKÁ, Jan BALVAN, Jaromír GUMULEC et. al.

Základní údaje

Originální název

Effect of prostate cancer cell line supernatant on functional polarization in macrophages

Autoři

MAZALOVA, L. (203 Česká republika), Z. SLADEK (203 Česká republika, garant), Martina RAUDENSKÁ (203 Česká republika, domácí), Jan BALVAN (203 Česká republika, domácí), Jaromír GUMULEC (203 Česká republika, domácí) a Michal MASAŘÍK (203 Česká republika, domácí)

Vydání

Bratislava Medical Journal - Bratislavské lekárske listy, BRATISLAVA, Univerzita Komenského, 2018, 0006-9248

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30105 Physiology

Stát vydavatele

Slovensko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 0.859

Kód RIV

RIV/00216224:14110/18:00104058

Organizační jednotka

Lékařská fakulta

DOI

UT WoS

000444369100011

Klíčová slova anglicky

prostate; cancer; supernatant; macrophages; cytokines

Štítky

Příznaky

Mezinárodní význam, Recenzováno
Změněno: 2. 5. 2019 14:10, Soňa Böhmová

Anotace

V originále

OBJECTIVES: We aimed on effect of supernatant derived from prostate cancer cell line PC-3 on M1/M2 functional polarization in macrophages. BACKGROUND: Cytokines play an important role in carcinogenesis. Most of them are produced by macrophages. Macrophages are divided into groups M1 or M2. Classical phenotype macrophages M1 support pro-inflammatory effects and produce pro-inflammatory cytokines, such as interleukin 6 (IL-6), interleukin 12 (IL-12), tumor necrosis factor alpha (TNF-alpha). Macrophages exhibiting a phenotype M2 secrete anti-inflammatory cytokines, e.g. interleukin 10 (IL-10), transforming growth factor beta (TGF-beta). METHODS: Peripheral blood monocytes were cultivated for 7 days and during this time went through a differentiation into macrophages. Macrophages were stimulated for 24 hours by lipopolysaccharide (LPS) as a positive control and cultivated with supernatant for another 24 hours. RESULTS: Macrophages cultivated without LPS and without supernatant were used as negative control. Relative expression of IL-6, IL-10, IL-12 and TNF-alpha was measured by Quantitative real-time PCR. Expression of pro-inflammatory cytokines was lower in macrophages with supernatant compared to positive control. CONCLUSION: Expression of pro-inflammatory cytokines was lower in macrophages with supernatant (M phi+sup) compared to positive control (M phi+LPS). Effect of the supernatant on expression of IL-6, IL-10, IL-12 and TNF-alpha was not confirmed (Tab. 1, Fig. 5, Ref. 15). Text in PDF www.elis.sk.