Identification of bacterial uropathogens by preparative
isoelectric focusing and matrix-assisted laser
desorption/ionization time-of-flight mass spectrometry

J 2018

Identification of bacterial uropathogens by preparative isoelectric focusing and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

SALPLACHTA, Jiri, Marie HORKA, Filip RŮŽIČKA and Karel SLAIS

Basic information

Original name

Identification of bacterial uropathogens by preparative isoelectric focusing and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Authors

SALPLACHTA, Jiri (203 Czech Republic, guarantor), Marie HORKA (203 Czech Republic), Filip RŮŽIČKA (203 Czech Republic, belonging to the institution) and Karel SLAIS (203 Czech Republic)

Edition

Journal of Chromatography A, Amsterdam, Netherlands, Elsevier, 2018, 0021-9673

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10606 Microbiology

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 3.858

RIV identification code

RIV/00216224:14110/18:00106895

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1016/j.chroma.2017.11.072

UT WoS

000423894500026

Keywords in English

Bacteria; Infection; Isoelectric focusing; Mass spectrometry; Preparative; Urine

Abstract

V originále

This study describes a new method for simultaneous identification of uropathogens in the case of poly bacterial urinary tract infections. The method utilizes recently developed preparative isoelectric focusing (IEF) in cellulose-based separation medium with a subsequent analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Preparative IEF was successfully used for both purification and separation of bacteria, Escherichia coli (pI 4.6) and Staphylococcus aureus (pI 3.4), in urine samples. The focused zones of bacteria, localized by the positions of focused colored pI markers, were easily collected from the separation media after the IEF analysis and then unambiguously identified by MALDI-TOF MS. The proposed method enables the identification of bacteria in urine specimens when the concentration of individual bacteria is >= 10(4) cells mL(-1). Another benefit is the viability of bacteria extracted from the collected fractions after preparative IEF. (C) 2017 Elsevier B.V. All rights reserved.

Links

NV16-29916A, research and development project

Name: Využití bakteriofágů v léčbě nozokomiálních infekcí spojených s multirezistencí či tvorbou biofilmu

VI20172020069, research and development project

Name: Vývoj moderních instrumentálních metod pro rychlou detekci a identifikaci vybraných B-agens a toxinů (Acronym: VMIMRDIBT)

Investor: Ministry of the Interior of the CR

Citovat

SALPLACHTA, Jiri, Marie HORKA, Filip RŮŽIČKA and Karel SLAIS. Identification of bacterial uropathogens by preparative isoelectric focusing and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Journal of Chromatography A. Amsterdam, Netherlands: Elsevier, 2018, vol. 1532, JAN 12 2018, p. 232-237. ISSN 0021-9673. Available from: https://dx.doi.org/10.1016/j.chroma.2017.11.072.

@article{1450498,
   author = {Salplachta, Jiri and Horka, Marie and Růžička, Filip and Slais, Karel},
   article_location = {Amsterdam, Netherlands},
   article_number = {JAN 12 2018},
   doi = {http://dx.doi.org/10.1016/j.chroma.2017.11.072},
   keywords = {Bacteria; Infection; Isoelectric focusing; Mass spectrometry; Preparative; Urine},
   language = {eng},
   issn = {0021-9673},
   journal = {Journal of Chromatography A},
   title = {Identification of bacterial uropathogens by preparative isoelectric focusing and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry},
   volume = {1532},
   year = {2018}
}

TY  - JOUR
ID  - 1450498
AU  - Salplachta, Jiri - Horka, Marie - Růžička, Filip - Slais, Karel
PY  - 2018
TI  - Identification of bacterial uropathogens by preparative isoelectric focusing and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
JF  - Journal of Chromatography A
VL  - 1532
IS  - JAN 12 2018
SP  - 232-237
EP  - 232-237
PB  - Elsevier
SN  - 00219673
KW  - Bacteria
KW  - Infection
KW  - Isoelectric focusing
KW  - Mass spectrometry
KW  - Preparative
KW  - Urine
N2  - This study describes a new method for simultaneous identification of uropathogens in the case of poly bacterial urinary tract infections. The method utilizes recently developed preparative isoelectric focusing (IEF) in cellulose-based separation medium with a subsequent analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Preparative IEF was successfully used for both purification and separation of bacteria, Escherichia coli (pI 4.6) and Staphylococcus aureus (pI 3.4), in urine samples. The focused zones of bacteria, localized by the positions of focused colored pI markers, were easily collected from the separation media after the IEF analysis and then unambiguously identified by MALDI-TOF MS. The proposed method enables the identification of bacteria in urine specimens when the concentration of individual bacteria is >= 10(4) cells mL(-1). Another benefit is the viability of bacteria extracted from the collected fractions after preparative IEF. (C) 2017 Elsevier B.V. All rights reserved.
ER  -

SALPLACHTA, Jiri, Marie HORKA, Filip RŮŽIČKA and Karel SLAIS. Identification of bacterial uropathogens by preparative isoelectric focusing and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. \textit{Journal of Chromatography A}. Amsterdam, Netherlands: Elsevier, 2018, vol.~1532, JAN 12 2018, p.~232-237. ISSN~0021-9673. Available from: https://dx.doi.org/10.1016/j.chroma.2017.11.072.

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