Impact of humic acid on the accumulation of metals by
microalgae

J 2018

Impact of humic acid on the accumulation of metals by microalgae

KOVACIK, Jozef, Marek BUJDOS a Petr BABULA

Základní údaje

Originální název

Impact of humic acid on the accumulation of metals by microalgae

Autoři

KOVACIK, Jozef (703 Slovensko, garant), Marek BUJDOS (703 Slovensko) a Petr BABULA (203 Česká republika, domácí)

Vydání

Environmental Science and Pollution Research, Heidelberg, Springer Heidelberg, 2018, 0944-1344

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30105 Physiology

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.914

Kód RIV

RIV/00216224:14110/18:00104103

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1007/s11356-018-1362-2

UT WoS

000429810200056

Klíčová slova anglicky

Bioaccumulation; Fluorescence microscopy; Heavy metals; Oxidative stress

Štítky

14110515, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 10. 2. 2019 14:38, Soňa Böhmová

Anotace

V originále

Indirect impact of humic acid (HA) on metal accumulation and toxicity (Cd, Ni, Pb, and Hg; 100 mu M; 24 h of exposure) in Scenedesmus quadricauda was studied. Algae were pre-cultured on solid (10 and 100 mg HA/L) or in liquid media (1, 5, and 10 mg HA/L) over 30 days and then exposed to metals mentioned above. Accumulation of applied metals irrespective of pre-culture increased in the order Ni < Cd < Pb < Hg. Algae pre-cultured on solid HA-enriched media accumulated more Cd (+ 46% at 10 mg HA/L), Ni (+ 50 and + 81% at 10 and 100 mg HA/L, respectively), and Pb (+ 15% at 100 mg HA/L) but the impact on Hg amount was not detected. Potassium and calcium decreased in response to all metals (K strongly under Hg excess) and HA had negligible impact. Interestingly, fluorescence microscopy detection of reactive oxygen species/nitric oxide (ROS/NO) balance showed that HA pre-culture suppressed ROS signal and stimulated NO signal in response to Cd (indicating positive impact of HA) while ROS signal in Ni and Pb treatments rather increased but NO signal decreased as expected from elevated Ni and Pb accumulation. Hg had clearly the most toxic impact on the ROS/NO balance. Algae pre-cultured in liquid HA-enriched media showed significantly increased Ni accumulation only (+ 14% at a dose 10 mg HA/L). Present study for the first time showed that humic acid may indirectly affect accumulation of metals and that solid HA-enriched medium used for pre-culture is more suitable to increase accumulation of metals by algae.

Citovat

KOVACIK, Jozef, Marek BUJDOS a Petr BABULA. Impact of humic acid on the accumulation of metals by microalgae. Environmental Science and Pollution Research. Heidelberg: Springer Heidelberg, 2018, roč. 25, č. 11, s. 10792-10798. ISSN 0944-1344. Dostupné z: https://dx.doi.org/10.1007/s11356-018-1362-2.

@article{1452056,
   author = {Kovacik, Jozef and Bujdos, Marek and Babula, Petr},
   article_location = {Heidelberg},
   article_number = {11},
   doi = {http://dx.doi.org/10.1007/s11356-018-1362-2},
   keywords = {Bioaccumulation; Fluorescence microscopy; Heavy metals; Oxidative stress},
   language = {eng},
   issn = {0944-1344},
   journal = {Environmental Science and Pollution Research},
   title = {Impact of humic acid on the accumulation of metals by microalgae},
   volume = {25},
   year = {2018}
}

TY  - JOUR
ID  - 1452056
AU  - Kovacik, Jozef - Bujdos, Marek - Babula, Petr
PY  - 2018
TI  - Impact of humic acid on the accumulation of metals by microalgae
JF  - Environmental Science and Pollution Research
VL  - 25
IS  - 11
SP  - 10792-10798
EP  - 10792-10798
PB  - Springer Heidelberg
SN  - 09441344
KW  - Bioaccumulation
KW  - Fluorescence microscopy
KW  - Heavy metals
KW  - Oxidative stress
N2  - Indirect impact of humic acid (HA) on metal accumulation and toxicity (Cd, Ni, Pb, and Hg; 100 mu M; 24 h of exposure) in Scenedesmus quadricauda was studied. Algae were pre-cultured on solid (10 and 100 mg HA/L) or in liquid media (1, 5, and 10 mg HA/L) over 30 days and then exposed to metals mentioned above. Accumulation of applied metals irrespective of pre-culture increased in the order Ni < Cd < Pb < Hg. Algae pre-cultured on solid HA-enriched media accumulated more Cd (+ 46% at 10 mg HA/L), Ni (+ 50 and + 81% at 10 and 100 mg HA/L, respectively), and Pb (+ 15% at 100 mg HA/L) but the impact on Hg amount was not detected. Potassium and calcium decreased in response to all metals (K strongly under Hg excess) and HA had negligible impact. Interestingly, fluorescence microscopy detection of reactive oxygen species/nitric oxide (ROS/NO) balance showed that HA pre-culture suppressed ROS signal and stimulated NO signal in response to Cd (indicating positive impact of HA) while ROS signal in Ni and Pb treatments rather increased but NO signal decreased as expected from elevated Ni and Pb accumulation. Hg had clearly the most toxic impact on the ROS/NO balance. Algae pre-cultured in liquid HA-enriched media showed significantly increased Ni accumulation only (+ 14% at a dose 10 mg HA/L). Present study for the first time showed that humic acid may indirectly affect accumulation of metals and that solid HA-enriched medium used for pre-culture is more suitable to increase accumulation of metals by algae.
ER  -

KOVACIK, Jozef, Marek BUJDOS a Petr BABULA. Impact of humic acid on the accumulation of metals by microalgae. \textit{Environmental Science and Pollution Research}. Heidelberg: Springer Heidelberg, 2018, roč.~25, č.~11, s.~10792-10798. ISSN~0944-1344. Dostupné z: https://dx.doi.org/10.1007/s11356-018-1362-2.

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