Gene target selection for loop-mediated isothermal
amplification for rapid discrimination of Treponema pallidum
subspecies

J 2018

Gene target selection for loop-mediated isothermal amplification for rapid discrimination of Treponema pallidum subspecies

KNAUF, Sascha, Simone LUERT, David ŠMAJS, Michal STROUHAL, Idrissa S. CHUMA et. al.

Základní údaje

Originální název

Gene target selection for loop-mediated isothermal amplification for rapid discrimination of Treponema pallidum subspecies

Autoři

KNAUF, Sascha (276 Německo, garant), Simone LUERT (276 Německo), David ŠMAJS (203 Česká republika, domácí), Michal STROUHAL (203 Česká republika, domácí), Idrissa S. CHUMA (276 Německo), Sieghard FRISCHMANN (276 Německo) a Mohammed BAKHEIT (276 Německo)

Vydání

PLoS neglected tropical diseases, San Francisco, Public Library of Science, 2018, 1935-2735

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30309 Tropical medicine

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 4.487

Kód RIV

RIV/00216224:14110/18:00104105

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1371/journal.pntd.0006396

UT WoS

000433487700036

Klíčová slova anglicky

gene target

Štítky

14110513, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 10. 2. 2019 13:08, Soňa Böhmová

Anotace

V originále

We show proof of concept for gene targets (polA, tprL, and TP_0619) that can be used in loop-mediated isothermal amplification (LAMP) assays to rapidly differentiate infection with any of the three Treponema pallidum subspecies (pallidum (TPA), pertenue (TPE), and endemicum (TEN)) and which are known to infect humans and nonhuman primates (NHPs). Four TPA, six human, and two NHP TPE strains, as well as two human TEN strains were used to establish and validate the LAMP assays. All three LAMP assays were highly specific for the target DNA. Amplification was rapid (5-15 min) and within a range of 10E+6 to 10E+2 of target DNA molecules. Performance in NHP clinical samples was similar to the one seen in human TPE strains. The newly designed LAMP assays provide proof of concept for a diagnostic tool that enhances yaws clinical diagnosis. It is highly specific for the target DNA and does not require expensive laboratory equipment. Test results can potentially be interpreted with the naked eye, which makes it suitable for the use in remote clinical settings.

Citovat

KNAUF, Sascha, Simone LUERT, David ŠMAJS, Michal STROUHAL, Idrissa S. CHUMA, Sieghard FRISCHMANN a Mohammed BAKHEIT. Gene target selection for loop-mediated isothermal amplification for rapid discrimination of Treponema pallidum subspecies. PLoS neglected tropical diseases. San Francisco: Public Library of Science, 2018, roč. 12, č. 4, s. 1-14. ISSN 1935-2735. Dostupné z: https://dx.doi.org/10.1371/journal.pntd.0006396.

@article{1452178,
   author = {Knauf, Sascha and Luert, Simone and Šmajs, David and Strouhal, Michal and Chuma, Idrissa S. and Frischmann, Sieghard and Bakheit, Mohammed},
   article_location = {San Francisco},
   article_number = {4},
   doi = {http://dx.doi.org/10.1371/journal.pntd.0006396},
   keywords = {gene target},
   language = {eng},
   issn = {1935-2735},
   journal = {PLoS neglected tropical diseases},
   title = {Gene target selection for loop-mediated isothermal amplification for rapid discrimination of Treponema pallidum subspecies},
   volume = {12},
   year = {2018}
}

TY  - JOUR
ID  - 1452178
AU  - Knauf, Sascha - Luert, Simone - Šmajs, David - Strouhal, Michal - Chuma, Idrissa S. - Frischmann, Sieghard - Bakheit, Mohammed
PY  - 2018
TI  - Gene target selection for loop-mediated isothermal amplification for rapid discrimination of Treponema pallidum subspecies
JF  - PLoS neglected tropical diseases
VL  - 12
IS  - 4
SP  - 1-14
EP  - 1-14
PB  - Public Library of Science
SN  - 19352735
KW  - gene target
N2  - We show proof of concept for gene targets (polA, tprL, and TP_0619) that can be used in loop-mediated isothermal amplification (LAMP) assays to rapidly differentiate infection with any of the three Treponema pallidum subspecies (pallidum (TPA), pertenue (TPE), and endemicum (TEN)) and which are known to infect humans and nonhuman primates (NHPs). Four TPA, six human, and two NHP TPE strains, as well as two human TEN strains were used to establish and validate the LAMP assays. All three LAMP assays were highly specific for the target DNA. Amplification was rapid (5-15 min) and within a range of 10E+6 to 10E+2 of target DNA molecules. Performance in NHP clinical samples was similar to the one seen in human TPE strains. The newly designed LAMP assays provide proof of concept for a diagnostic tool that enhances yaws clinical diagnosis. It is highly specific for the target DNA and does not require expensive laboratory equipment. Test results can potentially be interpreted with the naked eye, which makes it suitable for the use in remote clinical settings.
ER  -

KNAUF, Sascha, Simone LUERT, David ŠMAJS, Michal STROUHAL, Idrissa S. CHUMA, Sieghard FRISCHMANN a Mohammed BAKHEIT. Gene target selection for loop-mediated isothermal amplification for rapid discrimination of Treponema pallidum subspecies. \textit{PLoS neglected tropical diseases}. San Francisco: Public Library of Science, 2018, roč.~12, č.~4, s.~1-14. ISSN~1935-2735. Dostupné z: https://dx.doi.org/10.1371/journal.pntd.0006396.

Podrobný výpis o publikaci