| KOVÁČIK, Jozef, Sławomir DRESLER, Viera PETERKOVÁ a Petr BABULA. Metal-induced oxidative stress in terrestrial macrolichens. Chemosphere. OXFORD: PERGAMON-ELSEVIER SCIENCE LTD, 2018, roč. 203, JUL 2018, s. 402-409. ISSN 0045-6535. Dostupné z: https://dx.doi.org/10.1016/j.chemosphere.2018.03.112. |
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@article{1454396,
author = {Kováčik, Jozef and Dresler, Sławomir and Peterková, Viera and Babula, Petr},
article_location = {OXFORD},
article_number = {JUL 2018},
doi = {http://dx.doi.org/10.1016/j.chemosphere.2018.03.112},
keywords = {Antioxidants; Bioaccumulation; Fluorescence microscopy; Heavy metals; Oxidative stress; Reactive oxygen species (ROS)},
language = {eng},
issn = {0045-6535},
journal = {Chemosphere},
title = {Metal-induced oxidative stress in terrestrial macrolichens},
volume = {203},
year = {2018}
}
TY - JOUR
ID - 1454396
AU - Kováčik, Jozef - Dresler, Sławomir - Peterková, Viera - Babula, Petr
PY - 2018
TI - Metal-induced oxidative stress in terrestrial macrolichens
JF - Chemosphere
VL - 203
IS - JUL 2018
SP - 402-409
EP - 402-409
PB - PERGAMON-ELSEVIER SCIENCE LTD
SN - 00456535
KW - Antioxidants
KW - Bioaccumulation
KW - Fluorescence microscopy
KW - Heavy metals
KW - Oxidative stress
KW - Reactive oxygen species (ROS)
N2 - Short-term (24 h) responses of Cladonia arbuscula subsp. mitis and Cladonia furcate to copper (CuII) or chromium (CrIII) excess (10 or 100 mu M) were compared. C arbuscula accumulated more Cu and Cr at higher metal doses but both species revealed depletion of K and/or Ca amount. Not only Cu but also Cr typically elevated reactive oxygen species (ROS) formation (fluorescence microscopy detection of total ROS and hydrogen peroxide) and depleted nitric oxide (NO) signal, with Cu showing more negative impact on lipid peroxidation (BODIPY 581/591 C11 staining reagent). Metals and staining reagents also affected anatomical responses and photobiont/mycobiont visibility. Principally different impact of Cu and Cr was observed at antioxidative metabolites level, indicating various ways of metal-induced ROS removal and/or metal chelation: Cu strongly depleted glutathione (GSH) and stimulated phytochelatin 2 (PC2) content while ascorbic acid accumulation was depleted by Cu and stimulated by Cr. Subsequent experiment with GSH biosynthetic inhibitor (buthionine sulfoximine, BSO) revealed that 48 h of exposure is needed to deplete GSH and BSO-induced depletion of GSH and PC2 amounts under Cu or Cr excess elevated ROS but depleted NO. These data suggest close relations between thiols, NO and appearance of oxidative stress (ROS generation) under metallic stress also in lichens. (C) 2018 Elsevier Ltd. All rights reserved.
ER -
KOVÁČIK, Jozef, Sławomir DRESLER, Viera PETERKOVÁ a Petr BABULA. Metal-induced oxidative stress in terrestrial macrolichens. \textit{Chemosphere}. OXFORD: PERGAMON-ELSEVIER SCIENCE LTD, 2018, roč.~203, JUL 2018, s.~402-409. ISSN~0045-6535. Dostupné z: https://dx.doi.org/10.1016/j.chemosphere.2018.03.112.
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