Metal-induced oxidative stress in terrestrial macrolichens

J 2018

Metal-induced oxidative stress in terrestrial macrolichens

KOVÁČIK, Jozef, Sławomir DRESLER, Viera PETERKOVÁ a Petr BABULA

Základní údaje

Originální název

Metal-induced oxidative stress in terrestrial macrolichens

Autoři

KOVÁČIK, Jozef (703 Slovensko, garant), Sławomir DRESLER (616 Polsko), Viera PETERKOVÁ (703 Slovensko) a Petr BABULA (203 Česká republika, domácí)

Vydání

Chemosphere, OXFORD, PERGAMON-ELSEVIER SCIENCE LTD, 2018, 0045-6535

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30105 Physiology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 5.108

Kód RIV

RIV/00216224:14110/18:00104164

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1016/j.chemosphere.2018.03.112

UT WoS

000432235000046

Klíčová slova anglicky

Antioxidants; Bioaccumulation; Fluorescence microscopy; Heavy metals; Oxidative stress; Reactive oxygen species (ROS)

Štítky

14110515, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 10. 2. 2019 16:00, Soňa Böhmová

Anotace

V originále

Short-term (24 h) responses of Cladonia arbuscula subsp. mitis and Cladonia furcate to copper (CuII) or chromium (CrIII) excess (10 or 100 mu M) were compared. C arbuscula accumulated more Cu and Cr at higher metal doses but both species revealed depletion of K and/or Ca amount. Not only Cu but also Cr typically elevated reactive oxygen species (ROS) formation (fluorescence microscopy detection of total ROS and hydrogen peroxide) and depleted nitric oxide (NO) signal, with Cu showing more negative impact on lipid peroxidation (BODIPY 581/591 C11 staining reagent). Metals and staining reagents also affected anatomical responses and photobiont/mycobiont visibility. Principally different impact of Cu and Cr was observed at antioxidative metabolites level, indicating various ways of metal-induced ROS removal and/or metal chelation: Cu strongly depleted glutathione (GSH) and stimulated phytochelatin 2 (PC2) content while ascorbic acid accumulation was depleted by Cu and stimulated by Cr. Subsequent experiment with GSH biosynthetic inhibitor (buthionine sulfoximine, BSO) revealed that 48 h of exposure is needed to deplete GSH and BSO-induced depletion of GSH and PC2 amounts under Cu or Cr excess elevated ROS but depleted NO. These data suggest close relations between thiols, NO and appearance of oxidative stress (ROS generation) under metallic stress also in lichens. (C) 2018 Elsevier Ltd. All rights reserved.

Citovat

KOVÁČIK, Jozef, Sławomir DRESLER, Viera PETERKOVÁ a Petr BABULA. Metal-induced oxidative stress in terrestrial macrolichens. Chemosphere. OXFORD: PERGAMON-ELSEVIER SCIENCE LTD, 2018, roč. 203, JUL 2018, s. 402-409. ISSN 0045-6535. Dostupné z: https://dx.doi.org/10.1016/j.chemosphere.2018.03.112.

@article{1454396,
   author = {Kováčik, Jozef and Dresler, Sławomir and Peterková, Viera and Babula, Petr},
   article_location = {OXFORD},
   article_number = {JUL 2018},
   doi = {http://dx.doi.org/10.1016/j.chemosphere.2018.03.112},
   keywords = {Antioxidants; Bioaccumulation; Fluorescence microscopy; Heavy metals; Oxidative stress; Reactive oxygen species (ROS)},
   language = {eng},
   issn = {0045-6535},
   journal = {Chemosphere},
   title = {Metal-induced oxidative stress in terrestrial macrolichens},
   volume = {203},
   year = {2018}
}

TY  - JOUR
ID  - 1454396
AU  - Kováčik, Jozef - Dresler, Sławomir - Peterková, Viera - Babula, Petr
PY  - 2018
TI  - Metal-induced oxidative stress in terrestrial macrolichens
JF  - Chemosphere
VL  - 203
IS  - JUL 2018
SP  - 402-409
EP  - 402-409
PB  - PERGAMON-ELSEVIER SCIENCE LTD
SN  - 00456535
KW  - Antioxidants
KW  - Bioaccumulation
KW  - Fluorescence microscopy
KW  - Heavy metals
KW  - Oxidative stress
KW  - Reactive oxygen species (ROS)
N2  - Short-term (24 h) responses of Cladonia arbuscula subsp. mitis and Cladonia furcate to copper (CuII) or chromium (CrIII) excess (10 or 100 mu M) were compared. C arbuscula accumulated more Cu and Cr at higher metal doses but both species revealed depletion of K and/or Ca amount. Not only Cu but also Cr typically elevated reactive oxygen species (ROS) formation (fluorescence microscopy detection of total ROS and hydrogen peroxide) and depleted nitric oxide (NO) signal, with Cu showing more negative impact on lipid peroxidation (BODIPY 581/591 C11 staining reagent). Metals and staining reagents also affected anatomical responses and photobiont/mycobiont visibility. Principally different impact of Cu and Cr was observed at antioxidative metabolites level, indicating various ways of metal-induced ROS removal and/or metal chelation: Cu strongly depleted glutathione (GSH) and stimulated phytochelatin 2 (PC2) content while ascorbic acid accumulation was depleted by Cu and stimulated by Cr. Subsequent experiment with GSH biosynthetic inhibitor (buthionine sulfoximine, BSO) revealed that 48 h of exposure is needed to deplete GSH and BSO-induced depletion of GSH and PC2 amounts under Cu or Cr excess elevated ROS but depleted NO. These data suggest close relations between thiols, NO and appearance of oxidative stress (ROS generation) under metallic stress also in lichens. (C) 2018 Elsevier Ltd. All rights reserved.
ER  -

KOVÁČIK, Jozef, Sławomir DRESLER, Viera PETERKOVÁ a Petr BABULA. Metal-induced oxidative stress in terrestrial macrolichens. \textit{Chemosphere}. OXFORD: PERGAMON-ELSEVIER SCIENCE LTD, 2018, roč.~203, JUL 2018, s.~402-409. ISSN~0045-6535. Dostupné z: https://dx.doi.org/10.1016/j.chemosphere.2018.03.112.

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