Pre-operative Plasma miR-21-5p Is a Sensitive Biomarker and
Independent Prognostic Factor in Patients with Pancreatic
Ductal Adenocarcinoma Undergoing Surgical Resection

J 2018

Pre-operative Plasma miR-21-5p Is a Sensitive Biomarker and Independent Prognostic Factor in Patients with Pancreatic Ductal Adenocarcinoma Undergoing Surgical Resection

KARÁSEK, Petr, Natalia Anna GABŁO, Jan HLAVSA, Igor KISS, Petra VYCHYTILOVÁ et. al.

Základní údaje

Originální název

Pre-operative Plasma miR-21-5p Is a Sensitive Biomarker and Independent Prognostic Factor in Patients with Pancreatic Ductal Adenocarcinoma Undergoing Surgical Resection

Autoři

KARÁSEK, Petr (203 Česká republika), Natalia Anna GABŁO (616 Polsko, domácí), Jan HLAVSA (203 Česká republika, domácí), Igor KISS (203 Česká republika), Petra VYCHYTILOVÁ (203 Česká republika, domácí), Markéta HERMANOVÁ (203 Česká republika, domácí), Zdeněk KALA (203 Česká republika, domácí), Ondřej SLABÝ (203 Česká republika, domácí) a Vladimír PROCHÁZKA (203 Česká republika, garant, domácí)

Vydání

CANCER GENOMICS & PROTEOMICS, ATHENS, INT INST ANTICANCER RESEARCH, 2018, 1109-6535

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30204 Oncology

Stát vydavatele

Řecko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.147

Kód RIV

RIV/00216224:14110/18:00106939

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.21873/cgp.20090

UT WoS

000437932600010

Klíčová slova anglicky

Pancreatic ductal adenocarcinoma; microRNAs; plasma; miR-21-5p; diagnosis; prognosis

Štítky

14110112, 14110223, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 7. 3. 2019 16:35, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

Blood plasma microRNAs (miRNAs) are emerging as a clinically useful tool for non-invasive detection and prognosis estimation in various cancer types including pancreatic ductal adenocarcinoma (PDAC). The aim of the present study was to provide an independent validation of circulating miRNAs identified in previous studies as diagnostic and/or prognostic biomarkers in PDAC. Based on the literature search, 6 miRNAs were chosen as candidates for independent validation; miR-21-5p, miR-375, miR-155, miR-17-5p, miR-126-5p and miR-1290. Validation of these miRNAs was performed in a cohort of 25 patients with PDAC undergoing surgical resection and 24 healthy donors. Plasma levels of miRNAs were determined using quantitative real-time PCR. We confirmed significantly higher levels of all tested miRNA in blood plasma of PDAC patients in comparison to healthy controls with miR-21-5p showing the highest analytical performance (p<0.001; AUC>0.99). Increased levels of miR-21-5p (p=0.045) and miR-375 (p=0.013) were significantly associated with overall survival. Multivariate analysis demonstrated that miR-21-5p is a significant unfavorable prognostic factor independent on other clinical variables including adjuvant chemotherapy (hazard ratio 2.95; 95% CI 1.06-8.18; p=0.038). Our preliminary data indicate promising diagnostic and prognostic utility of plasma miR-21-5p in PDAC patients.

Návaznosti

NV16-31314A, projekt VaV

Název: Identifikace cirkulujících mikroRNA umožňujících prognostickou stratifikaci a selekci pacientů nejvíce profitujících z chirurgické léčby

Citovat

KARÁSEK, Petr, Natalia Anna GABŁO, Jan HLAVSA, Igor KISS, Petra VYCHYTILOVÁ, Markéta HERMANOVÁ, Zdeněk KALA, Ondřej SLABÝ a Vladimír PROCHÁZKA. Pre-operative Plasma miR-21-5p Is a Sensitive Biomarker and Independent Prognostic Factor in Patients with Pancreatic Ductal Adenocarcinoma Undergoing Surgical Resection. CANCER GENOMICS & PROTEOMICS. ATHENS: INT INST ANTICANCER RESEARCH, 2018, roč. 15, č. 4, s. 321-327. ISSN 1109-6535. Dostupné z: https://dx.doi.org/10.21873/cgp.20090.

@article{1454917,
   author = {Karásek, Petr and Gabło, Natalia Anna and Hlavsa, Jan and Kiss, Igor and Vychytilová, Petra and Hermanová, Markéta and Kala, Zdeněk and Slabý, Ondřej and Procházka, Vladimír},
   article_location = {ATHENS},
   article_number = {4},
   doi = {http://dx.doi.org/10.21873/cgp.20090},
   keywords = {Pancreatic ductal adenocarcinoma; microRNAs; plasma; miR-21-5p; diagnosis; prognosis},
   language = {eng},
   issn = {1109-6535},
   journal = {CANCER GENOMICS & PROTEOMICS},
   title = {Pre-operative Plasma miR-21-5p Is a Sensitive Biomarker and Independent Prognostic Factor in Patients with Pancreatic Ductal Adenocarcinoma Undergoing Surgical Resection},
   volume = {15},
   year = {2018}
}

TY  - JOUR
ID  - 1454917
AU  - Karásek, Petr - Gabło, Natalia Anna - Hlavsa, Jan - Kiss, Igor - Vychytilová, Petra - Hermanová, Markéta - Kala, Zdeněk - Slabý, Ondřej - Procházka, Vladimír
PY  - 2018
TI  - Pre-operative Plasma miR-21-5p Is a Sensitive Biomarker and Independent Prognostic Factor in Patients with Pancreatic Ductal Adenocarcinoma Undergoing Surgical Resection
JF  - CANCER GENOMICS & PROTEOMICS
VL  - 15
IS  - 4
SP  - 321-327
EP  - 321-327
PB  - INT INST ANTICANCER RESEARCH
SN  - 11096535
KW  - Pancreatic ductal adenocarcinoma
KW  - microRNAs
KW  - plasma
KW  - miR-21-5p
KW  - diagnosis
KW  - prognosis
N2  - Blood plasma microRNAs (miRNAs) are emerging as a clinically useful tool for non-invasive detection and prognosis estimation in various cancer types including pancreatic ductal adenocarcinoma (PDAC). The aim of the present study was to provide an independent validation of circulating miRNAs identified in previous studies as diagnostic and/or prognostic biomarkers in PDAC. Based on the literature search, 6 miRNAs were chosen as candidates for independent validation; miR-21-5p, miR-375, miR-155, miR-17-5p, miR-126-5p and miR-1290. Validation of these miRNAs was performed in a cohort of 25 patients with PDAC undergoing surgical resection and 24 healthy donors. Plasma levels of miRNAs were determined using quantitative real-time PCR. We confirmed significantly higher levels of all tested miRNA in blood plasma of PDAC patients in comparison to healthy controls with miR-21-5p showing the highest analytical performance (p<0.001; AUC>0.99). Increased levels of miR-21-5p (p=0.045) and miR-375 (p=0.013) were significantly associated with overall survival. Multivariate analysis demonstrated that miR-21-5p is a significant unfavorable prognostic factor independent on other clinical variables including adjuvant chemotherapy (hazard ratio 2.95; 95% CI 1.06-8.18; p=0.038). Our preliminary data indicate promising diagnostic and prognostic utility of plasma miR-21-5p in PDAC patients.
ER  -

KARÁSEK, Petr, Natalia Anna GABŁO, Jan HLAVSA, Igor KISS, Petra VYCHYTILOVÁ, Markéta HERMANOVÁ, Zdeněk KALA, Ondřej SLABÝ a Vladimír PROCHÁZKA. Pre-operative Plasma miR-21-5p Is a Sensitive Biomarker and Independent Prognostic Factor in Patients with Pancreatic Ductal Adenocarcinoma Undergoing Surgical Resection. \textit{CANCER GENOMICS \&{}amp; PROTEOMICS}. ATHENS: INT INST ANTICANCER RESEARCH, 2018, roč.~15, č.~4, s.~321-327. ISSN~1109-6535. Dostupné z: https://dx.doi.org/10.21873/cgp.20090.

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