Molecular characterization of Treponema pallidum subsp pallidum
in Switzerland and France with a new multilocus sequence typing
scheme

J 2018

Molecular characterization of Treponema pallidum subsp pallidum in Switzerland and France with a new multilocus sequence typing scheme

GRILLOVÁ, Linda, Tanika BAWA, Lenka PAŠTĚKOVÁ, Angele GAYET-AGERON, Kay NIESELT et. al.

Základní údaje

Originální název

Molecular characterization of Treponema pallidum subsp pallidum in Switzerland and France with a new multilocus sequence typing scheme

Autoři

GRILLOVÁ, Linda (203 Česká republika, domácí), Tanika BAWA (756 Švýcarsko), Lenka PAŠTĚKOVÁ (203 Česká republika, domácí), Angele GAYET-AGERON (756 Švýcarsko), Kay NIESELT (276 Německo), Michal STROUHAL (203 Česká republika, domácí), Patrice SEDNAOUI (250 Francie), Tristan FERRY (250 Francie), Matthias CAVASSINI (756 Švýcarsko), Stephan LAUTENSCHLAGER (756 Švýcarsko), Fabrizio DUTLY (756 Švýcarsko), Marta PLA-DIAZ (724 Španělsko), Michael KRUTZEN (756 Švýcarsko), Fernando GONZALEZ-CANDELAS (724 Španělsko), Homayoun C. BAGHERI (724 Španělsko), David ŠMAJS (203 Česká republika, domácí), Natascha ARORA (756 Švýcarsko) a Philipp P. BOSSHARD (756 Švýcarsko, garant)

Vydání

Plos one, San Francisco, Public Library of Science, 2018, 1932-6203

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.776

Kód RIV

RIV/00216224:14110/18:00101284

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1371/journal.pone.0200773

UT WoS

000440251600016

Klíčová slova anglicky

Treponema pallidum

Štítky

14110513, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 21. 3. 2019 12:20, Soňa Böhmová

Anotace

V originále

Syphilis is an important public health problem and an increasing incidence has been noted in recent years. Characterization of strain diversity through molecular data plays a critical role in the epidemiological understanding of this re-emergence. We here propose a new high-resolution multilocus sequence typing (MLST) scheme for Treponema pallidum subsp. pallidum (TPA). We analyzed 30 complete and draft TPA genomes obtained directly from clinical samples or from rabbit propagated strains to identify suitable typing loci and tested the new scheme on 120 clinical samples collected in Switzerland and France. Our analyses yielded three loci with high discriminatory power: TP0136, TP0548, and TP0705. Together with analysis of the 23S rRNA gene mutations for macrolide resistance, we propose these loci as MLST for TPA. Among clinical samples, 23 allelic profiles as well as a high percentage (80% samples) of macrolide resistance were revealed. The new MLST has higher discriminatory power compared to previous typing schemes, enabling distinction of TPA from other treponemal bacteria, distinction between the two main TPA clades (Nichols and SS14), and differentiation of strains within these clades.

Návaznosti

GA17-25455S, projekt VaV

Název: Studium genomů patogenních treponem na základě analýzy jednotlivých buněk

Investor: Grantová agentura ČR, Studium genomů patogenních treponem na základě analýzy jednotlivých buněk

GJ17-25589Y, projekt VaV

Název: Kmeny způsobující syfilis: jsou geneticky odlišné kmeny odlišné i v průběhu experimentální infekce?

Investor: Grantová agentura ČR, Genetically distinct groups of syphilis-causing strains: do they differ during experimental infection?

NV17-31333A, projekt VaV

Název: Vývoj nového typovacího systému pro původce syfilis, Treponema pallidum subsp. pallidum, zaměřeného na proteomické rozdíly

Citovat

GRILLOVÁ, Linda, Tanika BAWA, Lenka PAŠTĚKOVÁ, Angele GAYET-AGERON, Kay NIESELT, Michal STROUHAL, Patrice SEDNAOUI, Tristan FERRY, Matthias CAVASSINI, Stephan LAUTENSCHLAGER, Fabrizio DUTLY, Marta PLA-DIAZ, Michael KRUTZEN, Fernando GONZALEZ-CANDELAS, Homayoun C. BAGHERI, David ŠMAJS, Natascha ARORA a Philipp P. BOSSHARD. Molecular characterization of Treponema pallidum subsp pallidum in Switzerland and France with a new multilocus sequence typing scheme. Plos one. San Francisco: Public Library of Science, 2018, roč. 13, č. 7, s. 1-16. ISSN 1932-6203. Dostupné z: https://dx.doi.org/10.1371/journal.pone.0200773.

@article{1454936,
   author = {Grillová, Linda and Bawa, Tanika and Paštěková, Lenka and GayetandAgeron, Angele and Nieselt, Kay and Strouhal, Michal and Sednaoui, Patrice and Ferry, Tristan and Cavassini, Matthias and Lautenschlager, Stephan and Dutly, Fabrizio and PlaandDiaz, Marta and Krutzen, Michael and GonzalezandCandelas, Fernando and Bagheri, Homayoun C. and Šmajs, David and Arora, Natascha and Bosshard, Philipp P.},
   article_location = {San Francisco},
   article_number = {7},
   doi = {http://dx.doi.org/10.1371/journal.pone.0200773},
   keywords = {Treponema pallidum},
   language = {eng},
   issn = {1932-6203},
   journal = {Plos one},
   title = {Molecular characterization of Treponema pallidum subsp pallidum in Switzerland and France with a new multilocus sequence typing scheme},
   volume = {13},
   year = {2018}
}

TY  - JOUR
ID  - 1454936
AU  - Grillová, Linda - Bawa, Tanika - Paštěková, Lenka - Gayet-Ageron, Angele - Nieselt, Kay - Strouhal, Michal - Sednaoui, Patrice - Ferry, Tristan - Cavassini, Matthias - Lautenschlager, Stephan - Dutly, Fabrizio - Pla-Diaz, Marta - Krutzen, Michael - Gonzalez-Candelas, Fernando - Bagheri, Homayoun C. - Šmajs, David - Arora, Natascha - Bosshard, Philipp P.
PY  - 2018
TI  - Molecular characterization of Treponema pallidum subsp pallidum in Switzerland and France with a new multilocus sequence typing scheme
JF  - Plos one
VL  - 13
IS  - 7
SP  - 1-16
EP  - 1-16
PB  - Public Library of Science
SN  - 19326203
KW  - Treponema pallidum
N2  - Syphilis is an important public health problem and an increasing incidence has been noted in recent years. Characterization of strain diversity through molecular data plays a critical role in the epidemiological understanding of this re-emergence. We here propose a new high-resolution multilocus sequence typing (MLST) scheme for Treponema pallidum subsp. pallidum (TPA). We analyzed 30 complete and draft TPA genomes obtained directly from clinical samples or from rabbit propagated strains to identify suitable typing loci and tested the new scheme on 120 clinical samples collected in Switzerland and France. Our analyses yielded three loci with high discriminatory power: TP0136, TP0548, and TP0705. Together with analysis of the 23S rRNA gene mutations for macrolide resistance, we propose these loci as MLST for TPA. Among clinical samples, 23 allelic profiles as well as a high percentage (80% samples) of macrolide resistance were revealed. The new MLST has higher discriminatory power compared to previous typing schemes, enabling distinction of TPA from other treponemal bacteria, distinction between the two main TPA clades (Nichols and SS14), and differentiation of strains within these clades.
ER  -

GRILLOVÁ, Linda, Tanika BAWA, Lenka PAŠTĚKOVÁ, Angele GAYET-AGERON, Kay NIESELT, Michal STROUHAL, Patrice SEDNAOUI, Tristan FERRY, Matthias CAVASSINI, Stephan LAUTENSCHLAGER, Fabrizio DUTLY, Marta PLA-DIAZ, Michael KRUTZEN, Fernando GONZALEZ-CANDELAS, Homayoun C. BAGHERI, David ŠMAJS, Natascha ARORA a Philipp P. BOSSHARD. Molecular characterization of Treponema pallidum subsp pallidum in Switzerland and France with a new multilocus sequence typing scheme. \textit{Plos one}. San Francisco: Public Library of Science, 2018, roč.~13, č.~7, s.~1-16. ISSN~1932-6203. Dostupné z: https://dx.doi.org/10.1371/journal.pone.0200773.

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