Sequencing of Treponema pallidum subsp pallidum from isolate
UZ1974 using Anti-Treponemal Antibodies Enrichment: First
complete whole genome sequence obtained directly from human
clinical material

J 2018

Sequencing of Treponema pallidum subsp pallidum from isolate UZ1974 using Anti-Treponemal Antibodies Enrichment: First complete whole genome sequence obtained directly from human clinical material

GRILLOVÁ, Linda, Lorenzo GIACANI, Lenka PAŠTĚKOVÁ, Michal STROUHAL, Radim STRNADEL et. al.

Základní údaje

Originální název

Sequencing of Treponema pallidum subsp pallidum from isolate UZ1974 using Anti-Treponemal Antibodies Enrichment: First complete whole genome sequence obtained directly from human clinical material

Autoři

GRILLOVÁ, Linda (203 Česká republika, domácí), Lorenzo GIACANI (840 Spojené státy), Lenka PAŠTĚKOVÁ (203 Česká republika, domácí), Michal STROUHAL (203 Česká republika, domácí), Radim STRNADEL (203 Česká republika), Christina MARRA (840 Spojené státy), Arturo CENTURION-LARA (840 Spojené státy), Lucy POVEDA (756 Švýcarsko), Giancarlo RUSSO (756 Švýcarsko), Darina ČEJKOVÁ (203 Česká republika), Vladimír VAŠKŮ (203 Česká republika, domácí), Jan OPPELT (203 Česká republika, domácí) a David ŠMAJS (203 Česká republika, garant, domácí)

Vydání

Plos one, San Francisco, Public Library Science, 2018, 1932-6203

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.776

Kód RIV

RIV/00216224:14110/18:00101320

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1371/journal.pone.0202619

UT WoS

000442284500011

Klíčová slova anglicky

Treponema pallidum

Štítky

14110125, 14110513, CF BIOIT, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 21. 3. 2019 12:24, Soňa Böhmová

Anotace

V originále

Treponema pallidum subsp. pallidum (TPA) is the infectious agent of syphilis, a disease that infects more than 5 million people annually. Since TPA is an uncultivable bacterium, most of the information on TPA genetics comes from genome sequencing and molecular typing studies. This study presents the first complete TPA genome (without sequencing gaps) of clinical isolate (UZ1974), which was obtained directly from clinical material, without multiplication in rabbits. Whole genome sequencing was performed using a newly developed Anti-Treponemal Antibody Enrichment technique combined with previously reported Pooled Segment Genome Sequencing. We identified the UW074B genome, isolated from a sample previously propagated in rabbits, to be the closest relative of the UZ1974 genome and calculated the TPA mutation rate as 2.8 x 10(-10) per site per generation.

Návaznosti

GA17-25455S, projekt VaV

Název: Studium genomů patogenních treponem na základě analýzy jednotlivých buněk

Investor: Grantová agentura ČR, Studium genomů patogenních treponem na základě analýzy jednotlivých buněk

LM2015043, projekt VaV

Název: Česká infrastruktura pro integrativní strukturní biologii (Akronym: CIISB)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech Infrastructure for Integrative Structural Biology

NV17-31333A, projekt VaV

Název: Vývoj nového typovacího systému pro původce syfilis, Treponema pallidum subsp. pallidum, zaměřeného na proteomické rozdíly

Citovat

GRILLOVÁ, Linda, Lorenzo GIACANI, Lenka PAŠTĚKOVÁ, Michal STROUHAL, Radim STRNADEL, Christina MARRA, Arturo CENTURION-LARA, Lucy POVEDA, Giancarlo RUSSO, Darina ČEJKOVÁ, Vladimír VAŠKŮ, Jan OPPELT a David ŠMAJS. Sequencing of Treponema pallidum subsp pallidum from isolate UZ1974 using Anti-Treponemal Antibodies Enrichment: First complete whole genome sequence obtained directly from human clinical material. Plos one. San Francisco: Public Library Science, 2018, roč. 13, č. 8, s. 1-14. ISSN 1932-6203. Dostupné z: https://dx.doi.org/10.1371/journal.pone.0202619.

@article{1461497,
   author = {Grillová, Linda and Giacani, Lorenzo and Paštěková, Lenka and Strouhal, Michal and Strnadel, Radim and Marra, Christina and CenturionandLara, Arturo and Poveda, Lucy and Russo, Giancarlo and Čejková, Darina and Vašků, Vladimír and Oppelt, Jan and Šmajs, David},
   article_location = {San Francisco},
   article_number = {8},
   doi = {http://dx.doi.org/10.1371/journal.pone.0202619},
   keywords = {Treponema pallidum},
   language = {eng},
   issn = {1932-6203},
   journal = {Plos one},
   title = {Sequencing of Treponema pallidum subsp pallidum from isolate UZ1974 using Anti-Treponemal Antibodies Enrichment: First complete whole genome sequence obtained directly from human clinical material},
   volume = {13},
   year = {2018}
}

TY  - JOUR
ID  - 1461497
AU  - Grillová, Linda - Giacani, Lorenzo - Paštěková, Lenka - Strouhal, Michal - Strnadel, Radim - Marra, Christina - Centurion-Lara, Arturo - Poveda, Lucy - Russo, Giancarlo - Čejková, Darina - Vašků, Vladimír - Oppelt, Jan - Šmajs, David
PY  - 2018
TI  - Sequencing of Treponema pallidum subsp pallidum from isolate UZ1974 using Anti-Treponemal Antibodies Enrichment: First complete whole genome sequence obtained directly from human clinical material
JF  - Plos one
VL  - 13
IS  - 8
SP  - 1-14
EP  - 1-14
PB  - Public Library Science
SN  - 19326203
KW  - Treponema pallidum
N2  - Treponema pallidum subsp. pallidum (TPA) is the infectious agent of syphilis, a disease that infects more than 5 million people annually. Since TPA is an uncultivable bacterium, most of the information on TPA genetics comes from genome sequencing and molecular typing studies. This study presents the first complete TPA genome (without sequencing gaps) of clinical isolate (UZ1974), which was obtained directly from clinical material, without multiplication in rabbits. Whole genome sequencing was performed using a newly developed Anti-Treponemal Antibody Enrichment technique combined with previously reported Pooled Segment Genome Sequencing. We identified the UW074B genome, isolated from a sample previously propagated in rabbits, to be the closest relative of the UZ1974 genome and calculated the TPA mutation rate as 2.8 x 10(-10) per site per generation.
ER  -

GRILLOVÁ, Linda, Lorenzo GIACANI, Lenka PAŠTĚKOVÁ, Michal STROUHAL, Radim STRNADEL, Christina MARRA, Arturo CENTURION-LARA, Lucy POVEDA, Giancarlo RUSSO, Darina ČEJKOVÁ, Vladimír VAŠKŮ, Jan OPPELT a David ŠMAJS. Sequencing of Treponema pallidum subsp pallidum from isolate UZ1974 using Anti-Treponemal Antibodies Enrichment: First complete whole genome sequence obtained directly from human clinical material. \textit{Plos one}. San Francisco: Public Library Science, 2018, roč.~13, č.~8, s.~1-14. ISSN~1932-6203. Dostupné z: https://dx.doi.org/10.1371/journal.pone.0202619.

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